A5068361400- Cell Adhesion Molecules Research
- Cellular Mechanics and Interactions
- Protease and Inhibitor Mechanisms
- Polymer Surface Interaction Studies
- Bone Tissue Engineering Materials
- Peptidase Inhibition and Analysis
- Periodontal Regeneration and Treatments
- Molecular Biology Techniques and Applications
- Skin and Cellular Biology Research
- Bacterial biofilms and quorum sensing
- Monoclonal and Polyclonal Antibodies Research
- 3D Printing in Biomedical Research
- Graphene and Nanomaterials Applications
- Cancer Research and Treatments
- Heat shock proteins research
- Laser-Ablation Synthesis of Nanoparticles
- Orthopedic Infections and Treatments
- Tissue Engineering and Regenerative Medicine
- Dermatological and Skeletal Disorders
- IL-33, ST2, and ILC Pathways
- Anodic Oxide Films and Nanostructures
- Supramolecular Self-Assembly in Materials
- Cancer, Stress, Anesthesia, and Immune Response
- Bone Metabolism and Diseases
- Complement system in diseases
King's College London
2022-2023
Guy's Hospital
2022
University of Glasgow
2014-2021
Glasgow Life
2021
Universitat Politècnica de València
2011-2016
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine
2012
Researchers develop a simple technique to enhance the activity of growth factors during tissue healing.
Background The cell-material interaction is a complex bi-directional and dynamic process that mimics to certain extent the natural interactions of cells with extracellular matrix. Cells tend adhere rearrange adsorbed matrix (ECM) proteins on material surface in fibril-like pattern. Afterwards, ECM undergoes proteolytic degradation, which mechanism for removal excess usually approximated remodeling. remodeling consists two opposite events: assembly degradation. Methodology/Principal Findings...
Abstract While new biomaterials for regenerative therapies are being reported in the literature, clinical translation is slow. Some existing approaches rely on high doses of growth factors, such as bone morphogenetic protein‐2 (BMP‐2) regeneration, which can cause serious side effects. An ultralow‐dose factor technology described yielding bioactivity based a simple polymer, poly(ethyl acrylate) (PEA), and mechanisms to drive stem cell differentiation regeneration critical‐sized murine defect...
Abstract Laminins (LMs) are important structural proteins of the extracellular matrix (ECM). The abundance every LM isoform is tissue‐dependent, suggesting that has tissue‐specific roles. binds growth factors (GFs), which powerful cytokines widely used in tissue engineering due to their ability control stem cell differentiation. Currently, most commonly ECM mimetic material vitro Matrigel, a undefined composition containing and various GFs, but subjected batch variability lacking...
Base editing introduces precise single-nucleotide edits in genomic DNA and has the potential to treat genetic diseases such as blistering skin disease recessive dystrophic epidermolysis bullosa (RDEB), which is characterized by mutations COL7A1 gene type VII collagen (C7) deficiency. Adenine base editors (ABEs) convert A-T pairs G-C without requiring double-stranded breaks or donor templates. Here, we use ABE8e, a recently evolved ABE, correct primary RDEB patient fibroblasts harboring...
Osteogenic activity metabolites can be identified in nanovibrational culture and have their specificity potency enhanced.
Protein remodeling at the cell-material interface is an important phenomenon that should be incorporated into design of advanced biomaterials for tissue engineering. In this work, we address relationship between fibronectin (FN) activity material and remodeling, including proteolytic cascades. To do so, studied FN adsorption on two chemically similar substrates, poly(ethyl acrylate) (PEA) poly(methyl (PMA), which resulted in different distribution conformation protein interface: organized...
Engineering biomaterials for regenerative medicine involves a myriad of aspects to be considered the successful design, interaction with cells and integration living tissues (i.e. pore size, mechanical properties, degradation rate, biological activity). Among different technologies used functionalise synthetic promote cell adhesion, growth differentiation, this review focuses on strategies organise extracellular matrix (ECM) proteins in biomimetic way, as do natural vivo (the ECM is mesh...
Airway inflammation and remodelling are important pathophysiologic features in asthma other respiratory conditions. An intact epithelial cell layer is crucial to maintain lung homoeostasis, this depends on intercellular adhesion, whilst damaged epithelium the primary instigator of airway inflammation. The Coxsackievirus Adenovirus Receptor (CAR) highly expressed where it modulates cell-cell adhesion stability facilitates immune transepithelial migration. However, contribution CAR remains...
Abstract Fibronectin (FN) is a large glycoprotein which links and transmits signals between the cell's cytoskeleton extracellular matrix. FN organization into fibrils then fibrillogenesis can be induced with right substrate, such as poly(ethyl acrylate) (PEA), on becomes extended. Interestingly, almost identical polymer poly(methyl (PMA), has one less methylene bridge (─CH 2 ─), does not cause fibrillogenesis. To investigate difference in behavior PEA PMA, two substrates are modeled using...
Abstract The early onset of aggressive cutaneous squamous cell carcinoma (cSCC) and its rapid progression in recessive dystrophic epidermolysis bullosa (RDEB) leads to nearly 100% mortality rates these patients. Elevated levels phospho-STAT3 on tyrosine residue (STAT3Tyr705) have been intensively demonstrated RDEB-derived cSCC cells, demonstrating that the constitutive activation dysregulation Janus kinase (JAK)/STAT pathway plays a role RDEB pathogenesis. We efficiently applied ruxolitinib...
In article number 2010225, Manuel Salmeron-Sanchez and co-workers develop hydrogels that incorporate specific laminin isoforms. Laminins are ECM molecules tissue specific, promote integrin binding, sequester growth factors. The versatility of the system to engineer in vitro 3D cultures controlled stiffness mesenchymal stem cell differentiation (using LM 521) or neural 411) is demonstrated.
In article number 1800361, Matthew J. Dalby, Manuel Salmeron-Sanchez, and co-workers coat structural material, e.g., particles or scaffolds, with a bioactive synthetic material that organizes fibronectin to present BMP-2 high efficiency. This is used as novel regenerative approach heal critical-sized bone defects. Simultaneous integrin growth factor receptor synergistic signaling drives stem cell differentiation leading regeneration in the first veterinary patient. Image credit: Aleixandre...
Bioactive metabolites have wide-ranging biological activities and are a potential source of future research therapeutic tools. Here, we use nanovibrational stimulation to induce the osteogenic differentiation MSCs, in absence off-target differentiation. We show that this method, which does not rely on addition exogenous growth factors culture media, provides an artefact-free approach identifying bioactive specifically potently osteogenesis. first identify highly specific metabolite as...