A5004294874- Cellular Mechanics and Interactions
- Force Microscopy Techniques and Applications
- Advanced Fluorescence Microscopy Techniques
- 3D Printing in Biomedical Research
- 2D Materials and Applications
- Microfluidic and Bio-sensing Technologies
- Graphene research and applications
- Viral Infectious Diseases and Gene Expression in Insects
- Plant Water Relations and Carbon Dynamics
- Skin and Cellular Biology Research
- thermodynamics and calorimetric analyses
- Tree Root and Stability Studies
- Advanced Sensor and Energy Harvesting Materials
- Graphene and Nanomaterials Applications
- Polymer Surface Interaction Studies
- Molecular Junctions and Nanostructures
- Cancer Genomics and Diagnostics
- Wnt/β-catenin signaling in development and cancer
- Single-cell and spatial transcriptomics
- Chalcogenide Semiconductor Thin Films
- Ga2O3 and related materials
- MXene and MAX Phase Materials
- Skin Protection and Aging
- Advanced MEMS and NEMS Technologies
- Leaf Properties and Growth Measurement
Universität Ulm
2018-2023
Abstract Cellular membranes have long served as an inspiration for nanomaterial research. The preparation of ultrathin polydopamine (PDA) films with integrated protein pores containing phospholipids and embedded domain a membrane glycophorin A simplified cell mimics is reported. Large area, PDA are obtained by electropolymerization on gold surfaces 10–18 nm thickness dimensions up to 2.5 cm 2 . transferred from various other substrates such nylon mesh, silicon, or holes in the micrometer...
Skin is the largest human organ with easily noticeable biophysical manifestations of aging. As tissues age, there chronological accumulation changes due to internal and environmental factors. aging leads decreased elasticity loss dermal matrix integrity via degradation. The mechanical properties are maintained by fibroblasts, which undergo replicative may reach senescence. While secretory phenotype senescent fibroblasts well studied, little known about in phenotype. Therefore, we compare...
The actin cytoskeleton with its dynamic properties serves as the driving force for movement and division of cells gives cell shape structure. Disorders in occur many diseases. Deeper understanding regulation is essential order to better understand these biochemical processes. In our study, we use metal-induced energy transfer (MIET) a tool quantitatively examine rarely considered third dimension nanometer accuracy. particular, investigate influence different drugs acting on ROCK pathway...
Abstract Cellular senescence is a phenotype characterized by cessation of cell division, which can be caused exhaustive replication or environmental stress. It involved in age-related pathophysiological conditions and affects both the cellular cytoskeleton prime mechanosensors, focal adhesion complexes. While size adhesions increases during senescence, it unknown if how this accompanied remodeling internal structure. Our study uses metal-induced energy transfer to axial dimension proteins...
Bordered pit membranes of angiosperm xylem are anisotropic, mesoporous media between neighbouring conduits, with a key role in long distance water transport. Yet, their mechanical properties poorly understood. Here, we aim to quantify the stiffness intervessel over various growing seasons. By applying an AFM-based indentation technique "Quantitative Imaging" measured effective elastic modulus (
A photolithographic technique based on dry film photoresists for facile and low-cost patterning of microcantilever beams is presented. Dry enable instantly homogenous photoresist coatings flexible 3D patterned substrate surfaces, represented here by beams, which otherwise challenging if conventional spin-coating utilized. Compared to alternative microtechnologies, such as focused ion beam milling or resist spray coating, our strategy far less elaborate, fully compatible with routine additive...
We developed a reproducible micropatterning method to manipulate and normalize cell shape cell-cell separation on gold. used methoxy polyethylene glycol thiol (PEG-SH) create self-assembled monolayer that can be oxidized at desired shapes through photomask with deep UV light. The PEG coated extracellular matrix proteins seeded cells adopting the pre-defined shape. analyzed surfaces in wide range of biophysical applications.
Keratin filaments (KFs) comprise the intermediate of epithelial cells and are well known for their cytoprotective properties mechanical resilience. Although, several studies have demonstrated KFs' remarkable tensile relatively little is about acute implications stretch on KFs in living cells. This includes structural effects higher level assembly structures as posttranslational response mechanisms to possibly modify KF's properties. We subjected simple A549 lung 30% unidirectional already...
Upon aging, the function of intestinal epithelium declines with a concomitant increase in aging-related diseases. ISCs play an important role this process. It is known that ISC clonal dynamics follow neutral drift model. However, it not clear whether model still valid aged ISCs. Tracking by tracing revealed crypts into monoclonality substantially faster than young ones. experiments, vivo and ex vivo, implied similar expansion ability both Single-cell RNA sequencing for 1,920 high Lgr5 from...
For electronic and optical applications of two-dimensional (2D) materials their vertical heterostructures, it is important to know the positions, densities, atomic structures crystallographic defects. Thus, understand role these well-defined defects on properties 2D heterostructure devices, desirable combine device measurements with atomically resolved transmission electron microscopy (TEM) experiments. Here, beam used not only image but also create manipulate them. However, TEM poses...
Protein hydrogels represent ideal materials for advanced cell culture applications, including 3D-cultivation of even fastidious cells. Key properties fully functional and, at the same time, economically successful are excellent biocompatibility and fabrication processes allowing their easy production on a large scale based affordable compounds. Chemical crosslinking bovine serum albumin (BSA) with N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC) in water-in-oil emulsion...
Cells adapt their actin cytoskeletons architecture to structural cues of the environment in all three dimensions. Nevertheless, how manipulating cell shape influences z-dimension is unstudied, but crucial for an understanding mutual influence shape, tension and architecture. To study effect on cytoskeleton we combine metal-induced energy transfer as a super-resolution technique with micropatterning. This allows us not only precisely manipulate also regulate forces by changing while studying...
Cells actively probe the mechanical properties of their environment and adapt physiological state accordingly. For this fundamental task, they exert forces on using actomyosin machinery. This machinery consists protein fibers consisting dynamically assembling dissembling actin units, so called stress fibers, which motor proteins act to generate forces. is also used react external applied cells. Transduction from complexes extracellular space occurs via adaptive multi-protein complexes, focal...
Focal adhesions function are cellular anchoring points to the extracellular matrix and enable cells sense exert forces on their environment . They complex structures consisting of a multitude different proteins. Despite important role focal adhesion in adhesion, its structure mechanoresponse remain difficult resolve. Knowing exact position proteins under strain is necessary understand working principle. For detailed analysis architecture coupled with force response, we require method measure...
Abstract The actin cytoskeleton with its dynamic properties serves as the driving force for movement and division of cells gives cell shape structure. Disorders in occur many diseases. Deeper understanding regulation is essential order to better understand these biochemical processes. In our study, we use metal-induced energy transfer (MIET) a tool quantitatively examine rarely considered third dimension nanometer accuracy. particular, investigate influence different drugs acting on ROCK...
Cells react highly sensitive to mechanical and structural influences of their environment by mechanosensing. Micropatterning allows the precise regulation forces changing shape cell. In order record adaptation pattern changes in viscoelastic properties detail over a longer period time, VELOMIR sensor is ideally suited. The live cell measurements were recorded at 3000 frames per second 10 minutes. Here we show difference passive microrheology mouse embryonic fibroblasts with incorporated 1 μm...