A5091154406- Cellular Mechanics and Interactions
- Neonatal Respiratory Health Research
- Skin and Cellular Biology Research
- Cellular transport and secretion
- Inhalation and Respiratory Drug Delivery
- Ion channel regulation and function
- Hearing, Cochlea, Tinnitus, Genetics
- Erythrocyte Function and Pathophysiology
- Neuroscience of respiration and sleep
- Ion Channels and Receptors
- Microfluidic and Bio-sensing Technologies
- Silk-based biomaterials and applications
- Botulinum Toxin and Related Neurological Disorders
- Cardiac electrophysiology and arrhythmias
- Calcium signaling and nucleotide metabolism
- Hearing Loss and Rehabilitation
- Vestibular and auditory disorders
- 3D Printing in Biomedical Research
- Ophthalmology and Eye Disorders
- Clostridium difficile and Clostridium perfringens research
- Toxin Mechanisms and Immunotoxins
- Near-Field Optical Microscopy
- Receptor Mechanisms and Signaling
- Innovative Teaching Methods
- Protein Tyrosine Phosphatases
Universität Ulm
2011-2023
Nerviano Medical Sciences
2013
Innsbruck Medical University
2005-2006
University of Pennsylvania
2002-2005
Universität Innsbruck
1995-2001
Federal Institute for Health Protection of Consumers and Veterinary Medicine
2000
Homozygous zebrafish of the mutant relaxed ( red ts25 ) are paralyzed and die within days after hatching. A significant reduction intramembrane charge movements lack depolarization-induced but not caffeine-induced Ca 2+ transients suggested a defect in skeletal muscle dihydropyridine receptor (DHPR). Sequencing DHPR cDNAs indicated that α 1S subunit is normal, whereas β 1a harbors single point mutation resulting premature stop. Quantitative RT-PCR revealed mutated gene transcribed, Western...
Ca 2+ is considered a key element in multiple steps during regulated exocytosis. During the postfusion phase, an elevated cytoplasmic concentration ([Ca ]) c leads to fusion pore dilation. In neurons and neuroendocrine cells, this results from activation of voltage-gated channels plasma membrane. However, these are activated prefusion stage, little known about entry mechanisms stage. This may be particularly important for slow nonexcitable secretory cells. We recently described...
High-pressure freezing (HPF) is an electron microscopy (EM) preparation technique with superb ultrastructural preservation. Combined EM tomography it provides virtual serial sections extraordinary spatial resolution. For HPF, cells are usually cultured on a rigid sapphire disc that tight fit in the holding bracket of HPF apparatus. Since we using extensible elastic silicone membranes as growth support to perform cell stretch experiments, developed method clamp stretched membrane and place...
A key event in skeletal muscle activation is the rapid release of Ca(2+) from sarcoplasmic reticulum (SR), storage organelle cell. The surface membrane/transverse tubules and SR form functional units (calcium containing one or two couplons junctions), where voltage-sensing dihydropyridine receptor membrane interacts with channel [ryanodine (RyR)] depolarization cell converted into SR. Although RyR1 most important isoform muscle, some muscles also express high levels RyR3, an a wide tissue...
The C3 transferases from Clostridium botulinum (C3bot) and limosum (C3lim) mono-ADP-ribosylate thereby inactivate RhoA, -B -C of eukaryotic cells. Due to their extremely poor cellular uptake, were supposed be exoenzymes rather than exotoxins, challenging role in pathogenesis. Here, we report for the first time that low concentrations both C3lim C3bot are selectively internalized into macrophages/monocytes less 3 h, inducing reorganization actin cytoskeleton by ADP-ribosylation Rho. We...
Type II pneumocytes secrete surfactant, a lipoprotein‐like substance reducing the surface tension in lung, by regulated exocytosis of secretory vesicles termed lamellar bodies (LBs). This process is characterized protracted postfusion phase which fusion pores open slowly and may act as mechanical barriers for release. Combining dark‐field with fluorescence microscopy, we show ß‐actin green fluorescent protein‐transfected that LB plasma membrane followed actin coating fused LB. inhibited...
Mechanical stimuli control multiple cellular processes such as secretion, growth, and differentiation. A widely used method to investigate cell strain ex vivo is stretching an elastic membrane which cells adhere. However, simultaneous imaging of dynamic signals from single living grown on substrates during uni-axial changes length usually hampered by the movement sample along axis out narrow optical field view. We a thin, prestrained, chamber growth substrate for deformed with...
Mechanical forces affect biological systems in their natural environment a widespread manner. stress may either stimulate cells or even induce pathological processes. Cells sensing mechanical usually respond to such stressors with proliferation differentiation. Hence, for vitro studies, the ability impose controlled on combined appropriate analytical tools providing an immediate answer is essential understand fundamental Here, we present novel uniaxial motorized cell stretching device that...
CD95 is a multifunctional receptor that induces cell death or proliferation depending on the signal, type, and cellular context. Here, we describe thus far unknown function of as silencer T activation. Naive human cells triggered by antigen-presenting expressing membrane-bound form ligand (CD95L) stimulated anti-CD3 -CD28 antibodies in presence recombinant CD95L had reduced activation proliferation, whereas preactivated, CD95-sensitive underwent apoptosis. Triggering during priming...
Keratin filaments impart resilience against mechanical extension of the cell. Despite pathophysiological relevance this function, very little is known about properties intermediate in living cells and how these are modulated. We used keratin mutants that mimic or abrogate phosphorylation 8-serine(431) 18-serine(52) investigated their effect on tortuousness after cell stretch release squamous carcinoma cells. Cells transfected with wild-type keratins were as controls. can show...
Mechanical forces exert multiple effects in cells, ranging from altered protein expression patterns to cell damage and death. Despite undisputable biological importance, little is known about structural changes cells subjected strain ex vivo. Here, we undertake the first transmission electron microscopy investigation combined with fluorescence imaging on pulmonary alveolar type II that are equibiaxial strain. When investigated immediately after stretch, demonstrate curved cytokeratin (CK)...
Retrieval of information has substantially changed within the last two decades. Naturally, this also affected learning/teaching techniques, and methods that are commonly referred to as "e-learning" have become an important part in modern education. Institutions decide if (and how) implement new form teaching but face problem little subject-specific research been published for different modes methods. The present study compares a course module physiology laboratory medical students...
The outstanding properties of gold nanoparticles (NPs) make them very attractive for biomedical applications. In particular, the inhalation route has gained considerable interest as an innovative strategy diagnosis and treatment pulmonary diseases. It is, therefore, important to scrutinise potentially deleterious or side effects NPs on lung epithelium. present study investigates, first time, impact polyethylene glycol (PEG)-coated freshly purified primary cultures rat alveolar type II (ATII)...
During the COVID-19 pandemic, absence of in-person teaching was partially compensated for through videoconferencing. However, lecturers complain that students do not participate actively in video-based online seminars. One reason cited this is Zoom fatigue. Conferences virtual reality (VR), accessible with and without head-mounted display, represent one potential remedy to issue. The research date does shed any light on (1.) experience, (2.) student demand, (3.) learning experience...
Abstract Exocytosis is the intracellular trafficking step where a secretory vesicle fuses with plasma membrane to release content. Actin and microtubules both play role in exocytosis; however, their interplay not understood. Here we study interaction of actin during exocytosis lung alveolar type II (ATII) cells that secrete surfactant from large vesicles. Surfactant extrusion facilitated by an coat forms on shortly after fusion pore opening. compression allows hydrophobic be released...