A5013229048- Ion channel regulation and function
- Neuroscience and Neuropharmacology Research
- Cardiac electrophysiology and arrhythmias
- Cardiomyopathy and Myosin Studies
- Lipid Membrane Structure and Behavior
- Nicotinic Acetylcholine Receptors Study
- Receptor Mechanisms and Signaling
- Physiological and biochemical adaptations
- Neuroscience and Neural Engineering
- Cardiovascular Effects of Exercise
- Signaling Pathways in Disease
- Biotin and Related Studies
- Neurobiology and Insect Physiology Research
- Heart Rate Variability and Autonomic Control
- Advanced Fluorescence Microscopy Techniques
Universität Innsbruck
2020-2023
Innsbruck Medical University
2005-2023
Medical University of Graz
2016-2017
Leibniz Institute for Neurobiology
2011
University of Pennsylvania
2005
Homozygous zebrafish of the mutant relaxed ( red ts25 ) are paralyzed and die within days after hatching. A significant reduction intramembrane charge movements lack depolarization-induced but not caffeine-induced Ca 2+ transients suggested a defect in skeletal muscle dihydropyridine receptor (DHPR). Sequencing DHPR cDNAs indicated that α 1S subunit is normal, whereas β 1a harbors single point mutation resulting premature stop. Quantitative RT-PCR revealed mutated gene transcribed, Western...
In neurons L-type calcium currents function in gene regulation and synaptic plasticity, while excessive influx leads to excitotoxicity neurodegeneration. The major neuronal Ca V 1.2 channels are localized clusters dendritic shafts spines. Whereas remain stable during NMDA-induced depression, rapidly downregulated strong excitatory stimulation. Here we used fluorescence recovery after photobleaching (FRAP), live cell-labeling protocols, single particle tracking (SPT) analyze the turnover...
Auxiliary beta subunits modulate current properties and mediate the functional membrane expression of voltage-gated Ca(2+) channels in heterologous cells. In brain, all four isoforms are widely expressed, yet little is known about their specific roles neuronal functions. Here, we investigated targeting role Ca(V)1.2 alpha(1) cultured hippocampal neurons. Quantitative reverse transcription-PCR showed equal expression, immunofluorescence a similar distribution endogenous throughout dendrites...
In neurons L-type calcium currents contribute to synaptic plasticity and activity-dependent gene regulation. The subcellular localization of Ca V 1.2 its association with upstream downstream signaling proteins is important for efficient specific signal transduction. Here we tested the hypothesis that A-kinase anchoring (AKAPs) or PDZ-proteins are responsible targeting in postsynaptic compartment glutamatergic neurons. Double-immunofluorescence labeling hippocampal transfected external HA...
The β subunits of voltage-gated calcium channels regulate surface expression and gating Ca V 1 2 α thus contribute to neuronal excitability, neurotransmitter release, calcium-induced gene regulation. In addition, certain are targeted into the nucleus, where they interact directly with epigenetic machinery. Whereas their involvement in this multitude functions is reflected by a great molecular heterogeneity isoforms derived from four genes abundant alternative splicing, little known about...
VGCCs are multisubunit complexes that play a crucial role in neuronal signaling. Auxiliary α2δ subunits of modulate trafficking and biophysical properties the pore-forming α1 subunit trigger excitatory synaptogenesis. Alterations expression level were implicated several syndromes diseases, including chronic neuropathic pain, autism, epilepsy. However, contribution distinct to excitatory/inhibitory imbalance aberrant network connectivity characteristic for these pathologic conditions remains...
The functional separation between skeletal and cardiac muscles, which occurs at the threshold vertebrates invertebrates, involves evolution of separate contractile control proteins for two types striated as well mechanisms activation. link electrical excitation surface membrane activation material (known excitation–contraction [e–c] coupling) requires interaction a voltage sensor in membrane, dihydropyridine receptor (DHPR), calcium release channel sarcoplasmic reticulum, ryanodine (RyR)....
Voltage-gated calcium channels are multi-subunit membrane proteins which transduce depolarization into cellular functions like excitation-contraction coupling in muscle or neurotransmitter release neurons. The auxiliary β subunits function targeting of the channel and modulation its gating properties. However, whether can reversibly interact with, thus differentially modulate is still unresolved. Here we applied fluorescence recovery after photobleaching (FRAP) GFP-tagged α1 expressed...
L-type voltage-gated CaV1.2 calcium channels (CaV1.2) are key regulators of neuronal excitability, synaptic plasticity, and excitation-transcription coupling. Surface-exposed distributes in clusters along the dendrites hippocampal neurons. A permanent exchange between stably clustered laterally diffusive extra-clustered maintains steady-state levels at dendritic signaling domains. dynamic equilibrium anchored receptors is a common feature among ion crucial to modulate transduction. Despite...
Sepsis has emerged as a global health burden associated with multiple organ dysfunction and 20% mortality rate in patients. Numerous clinical studies over the past two decades have correlated disease severity septic patients impaired heart variability (HRV), consequence of chronotropic response sinoatrial node (SAN) pacemaker activity to vagal/parasympathetic stimulation. However, molecular mechanism(s) downstream parasympathetic inputs not been investigated yet sepsis, particularly SAN....