A5102005955- PI3K/AKT/mTOR signaling in cancer
- Neuroscience and Neuropharmacology Research
- Receptor Mechanisms and Signaling
- Protein Kinase Regulation and GTPase Signaling
- Gut microbiota and health
- Memory and Neural Mechanisms
- HER2/EGFR in Cancer Research
- Clostridium difficile and Clostridium perfringens research
- Ion channel regulation and function
- Glycosylation and Glycoproteins Research
- Gallbladder and Bile Duct Disorders
- Cardiac electrophysiology and arrhythmias
- Cytokine Signaling Pathways and Interactions
- Pancreatic and Hepatic Oncology Research
- Nitric Oxide and Endothelin Effects
- Cancer Mechanisms and Therapy
- Cancer therapeutics and mechanisms
- Angiogenesis and VEGF in Cancer
- Inflammatory mediators and NSAID effects
- DNA Repair Mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Pediatric Hepatobiliary Diseases and Treatments
- Gastric Cancer Management and Outcomes
- Inflammatory Bowel Disease
- Proteoglycans and glycosaminoglycans research
Huzhou Central Hospital
2017-2025
Huzhou University
2021-2025
Zhejiang University
2019-2024
University of Iowa
2009-2023
Jiangsu University
2012-2022
Affiliated Hospital of Jiangsu University
2019
Ningbo Medical Center Lihuili Hospital
2018
PLA Navy General Hospital
2014
Iowa State University
2006-2010
β-Adrenergic regulation of the L-type calcium channel Ca v 1.2 exhibits surprising differences in heart and brain.
Pharmacological experiments on Ascaris suum have demonstrated the presence of three (N-, L-, and B-) subtypes cholinergic receptor mediating contraction body wall muscle in parasitic nematodes. In present study, these ionotropic acetylcholine (ACh) receptors (nAChRs) were activated by levamisole bephenium under patch-clamp conditions competitively antagonized paraherquamide 2-desoxoparaherquamide. A number recordings exhibited separate current amplitude levels, indicating small,...
Sydney Brenner promoted Caenorhabditis elegans as a model organism, and subsequent investigations pursued resistance to the nicotinic anthelmintic drug levamisole in C. at genetic level. These studies have advanced our understanding of genes associated with neuromuscular transmission antinematodal drug. In lev-8 lev-1 mutant elegans, is reductions levamisole-activated whole muscle cell currents. Although are known code for acetylcholine receptor (nAChR) subunits, an explanation why these...
The widespread noradrenergic innervation in the brain promotes arousal and learning by molecular mechanisms that remain largely undefined. Recent work shows β(2)-adrenergic receptor (β(2)AR) is linked to AMPA-type glutamate subunit GluA1 via stargazin PSD-95 (Joiner ML, Lise MF, Yuen EY, Kam AY, Zhang M, Hall DD, Malik ZA, Qian H, Chen Y, Ulrich JD, Burette AC, Weinberg RJ, Law PY, El-Husseini A, Yan Z, Hell JW. EMBO J 29: 482-495, 2010). We now demonstrate β(2)AR plays a prominent role...
Recent evidence indicates that the A kinase anchor protein AKAP5 (AKAP79/150) interacts not only with PKA but also various adenylyl cyclase (AC) isoforms. However, physiological relevance of AC-AKAP5 binding is largely unexplored. We now show postsynaptic targeting AC by important for phosphorylation AMPA-type glutamate receptor subunit GluA1 on Ser-845 and synaptic plasticity. Phosphorylation strongly reduced (by 70%) under basal conditions in KO mice at all D36 mice, which site (i.e....
The roles of preoperative serum carcinoembryonic antigen (sCEA) and postoperative tissue (tCEA) have been extensively studied in isolation colorectal cancer (CRC). However, the combined role sCEA tCEA remains inadequately described. A total 1,757 retrospective cases stage 0-IV CRC from January 2006 to 2016 our institution were included. Clinicopathological features follow-up data collected. Stage 0 was with I. levels classified as normal or high (>10 ng/mL), while categorised into three...
Cisplatin exert its anticancer effect by creating intrastrand and interstrand DNA cross-links which block replication is a major drug used to treat lung cancer. However, the main obstacle of efficacy treatment resistance. Here, we show that expression translesion synthesis (TLS) polymerase Q (POLQ) was significantly elevated exposure cancer cells A549/DR (a cisplatin-resistant A549 cell line) cisplatin. POLQ correlated inversely with homologous recombination (HR) activity. Co-depletion BRCA2...
Although chemotherapeutic regimen containing gemcitabine is the first-line therapy for advanced lung squamous cell carcinoma (LSCC), resistance remains an important clinical problem. Some studies suggest that overexpressions of ribonucleotide reductase (RNR) subunit M2 (RRM2) may be involved in resistance. We used a novel RRM2 inhibitor, GW8510, as sensitization agent to investigate therapeutic utility reversing LSCC. Results showed expressions were increased intrinsic resistant LSCC cells...
Background Our previous research results showed that Type II cGMP dependent protein kinase (PKG II) could block the activation of epidermal growth factor receptor (EGFR) and consequently inhibit proliferation related MAPK/ERK-mediated signal transduction gastric cancer cell line BGC-823, suggesting PKG might other EGFR-triggered pathways biological activities cells. This paper was designed to investigate potential inhibition on EGF/EGFR-induced migration activity pathways....
Aim: This study aims to explore the effect of different doses antibiotics on rats in order observe alterations their fecal microbiota, inflammatory changes colonic mucosa and four types markers blood serum. Methods: Our methodology involved separating 84 female Sprague Dawley into groups A-G, with each group consisting 12 rats. We collected rat feces for analysis, using a distinct medium bacterial cultivation counting colonies under microscope. On 11th 15th days experiment, half from were...
Schistosomes are amongst the most important and neglected pathogens in world, schistosomiasis control relies almost exclusively on a single drug. The neuromuscular system of schistosomes is fertile ground for therapeutic intervention, yet details physiological events involved function remain largely unknown. Short amidated neuropeptides, FMRFamide-like peptides (FLPs), distributed abundantly throughout nervous every flatworm examined they produce potent myoexcitation. Our goal here was to...
Type II cyclic guanosine monophosphate (cGMP)-dependent protein kinase (PKG II) is a membrane-anchored enzyme expressed mainly in the intestinal mucosa and brain, associated with various physiological or pathological processes. Upregulation of PKG known to induce apoptosis inhibit proliferation metastasis cancer cells. The inhibitory effect has been shown be dependent on inhibition activation epidermal growth factor receptor (EGFR) blockade EGFR downstream signal transduction vitro. However,...
Cross-linked hyaluronic acid gel (CHAG) has been used to prevent postoperative adhesion of abdominal tumorectomy. However, its effect on tumor cells is still unknown. This paper was designed investigate the CHAG metastasis and growth cells. Migration invasion assays, Western blotting, pull down assay, siRNA interference, nude mice implantation model were applied in this study. The results vitro experiments with gastric cancer cell line AGS hepatic HepG2 showed that inhibited migration...
Our previous study revealed that Type II cGMP‑dependent protein kinase (PKG II) inhibits epidermal growth factor (EGF)‑induced MAPK/ERK and MAPK/JNK‑mediated signal transduction through the inhibition of phosphorylation/activation EGF receptor (EGFR). As EGFR also mediates several other pathways besides MAPK‑mediated pathways, present was designed to investigate whether PKG able inhibit EGF/EGFR‑induced phosphatidylinositol‑3‑kinase (PI3K)/Akt‑mediated transduction. The AGS human gastric...
It has been demonstrated that REV3, the catalytic subunit of translesion synthesis (TLS) polymerase ζ, play an important role in DNA damage response (DDR) induced by cisplatin, and Ataxia‐telangietasia mutated Rad‐3‐related (ATR) knase is a central player activating cell cycle checkpoint, stabilizing replication forks, regulating DDR, promoting repair caused cisplatin. Cancer cells deficient either one REV3 ATR are more sensitive to However, whether co‐inhibition can further increase...
Gefitinib, an EGFR-tyrosine kinase inhibitor, significantly improve prognosis in patients with advanced non-small cell lung cancer (NSCLC). The aim of this study was to evaluate the usefulness MUC1 and vascular endothelial growth factor (VEGF) mRNA expression peripheral blood as means predicting benefit from gefitinib therapy NSCLC patients.MUC1 VEGF expressions were detected 66 before (B0) 4 weeks after treatment (B4w) gefitinib, using real-time quantitative-PCR assay. Correlations between...