A5042950704- Neuropeptides and Animal Physiology
- Receptor Mechanisms and Signaling
- Pharmacological Receptor Mechanisms and Effects
- Hematopoietic Stem Cell Transplantation
- Neuroscience and Neuropharmacology Research
- Pain Mechanisms and Treatments
- Mesenchymal stem cell research
- Chemical Synthesis and Analysis
- Neurogenesis and neuroplasticity mechanisms
- MicroRNA in disease regulation
- Cancer, Stress, Anesthesia, and Immune Response
- RNA Research and Splicing
- Immunotherapy and Immune Responses
- Neuroblastoma Research and Treatments
- Protein Kinase Regulation and GTPase Signaling
- CAR-T cell therapy research
- Immune Cell Function and Interaction
- RNA Interference and Gene Delivery
- Signaling Pathways in Disease
- Virus-based gene therapy research
- Pancreatic function and diabetes
- RNA and protein synthesis mechanisms
- Chemokine receptors and signaling
- Histone Deacetylase Inhibitors Research
- T-cell and B-cell Immunology
University of Minnesota Medical Center
2012-2024
University of Duisburg-Essen
2020-2022
University of Minnesota
2011-2020
University of California, Los Angeles
1995-2020
American Academy of Forensic Sciences
2020
Westmead Hospital
2016-2018
St. Jude Children's Research Hospital
2014-2017
National Health Research Institutes
2017
National University Health System
2013-2014
National University Hospital
2005-2014
The G protein-coupled mu-opioid receptor (mu OR) mediates the physiological effects of endogenous opioid peptides as well structurally distinct alkaloids morphine and etorphine. An intriguing feature mu OR signaling is differential trafficking desensitization properties following activation by agonists, which have been proposed possible mechanisms related to tolerance. Here we report that ability agonists differentially regulate internalization their promote kinase (GRK)-dependent...
Given the mounting evidence for involvement of delta opioid receptors in tolerance and physical dependence mu receptor agonists, we have investigated possible interaction between by using bivalent ligands. Based on reports suppression antinociceptive antagonist naltrindole (NTI), ligands [mu-delta agonist-antagonist (MDAN) series] that contain different length spacers, pharmacophores derived from NTI agonist oxymorphone, been synthesized evaluated intracerebroventricular (i.c.v.)...
Chronic treatment of neuroblastoma X glioma NG108-15 hybrid cells with the opiate agonist etorphine resulted in a decrease both receptor density (receptor down-regulation) and ability to inhibit prostaglandin E1 (PGE1)-stimulated increases cyclic AMP levels desensitization). Opiate down-regulation desensitization were homologous as indicated by lack apparent change muscarinic, alpha 2-adrenergic, PGE1 binding also retention, albeit modulation, carbachol norepinephrine PGE1-stimulated after...
The cellular location of extracellular signal-regulated kinases (ERKs) activated by a G protein-coupled receptor was shown to be dependent on the pathway that mediated their activation. In general, fast activation ERKs (2 min) proteins resulted in nuclear translocation phosphorylated ERKs, whereas slower (10 β-arrestins cytosolic retention ERKs. However, we observed distinct differences from this established itinerary with μ-opioid receptor-activated Agonists such as morphine and methadone...
Abstract: The conditions in which Leu 5 ‐enkephalin inhibition of striatal adenylate cyclase was observed were defined. It determined that enkephalin dependent on GTP. apparent K m for GTP opiate to be 0.5 and 2 μM when 0.1 mM‐ mM‐ATP used as substrate. ITP, but not CTP or UTP, could substitute the reaction. Though addition monovalent cations—Na + ,K , Li Cs choline —stimulated activity, did require Na theophylline phosphodiesterase inhibitor. Under optimal conditions, i.e., 20 μM‐GTP 100...
Pericytes play a crucial role in angiogenesis and vascular maintenance. They can be readily identified vivo isolated as CD146(+)CD34(-) cells from various tissues. Whether these other markers reliably identify pericytes vitro is unclear. selected exhibit multilineage potential. Thus, their perivascular location might represent stem cell niche. This has spurred assumptions that not only all are mesenchymal stromal (MSCs), but also MSCs act pericytes. Considering this hypothesis, we developed...
One-pot multienzyme continuous cascade for the conversion of HMF to FDCA using HRP activate GOaseM<sub>3-5</sub> in situ.
Antisera were produced against synthetic peptides predicted from the recent cloning of a delta opioid receptor, DOR-1. Confocal miscroscopic examination immunostained spinal cord sections revealed that DOR-1 immunoreactive (-ir) nerve fibers and terminals form moderately dense plexus within superficial dorsal horn rats mice. These decreased dramatically following rhizotomy consistent with these observations population small diameter neurons in ganglia exhibited DOR-1-ir. ganglion also for...
PURPOSE The objective of this study was to characterize CD34+ cell grafts, obtained using a novel technique, from children undergoing autologous bone marrow transplantation (BMT) for cancer therapy. In particular, we wanted determine if the grafts generated hematopoietic reconstitution, since positive result would motivate further development and use methodology. PATIENTS AND METHODS This pilot feasibility clinical trial involved 13 patients < or = 25 years age with advanced solid tumors,...
Antisera were raised against a synthetic peptide corresponding to the carboxyl terminus of kappa-opioid receptor (KOR1). Specificity antisera was verified by staining COS-7 cells transfected with KOR1 and epitope-tagged cDNAs, recognition proteins on Western blots both brain tissue, absence tissue after preabsorption cognate peptide, strong correlation between distribution immunoreactivity that earlier ligand binding in situ hybridization studies. Results indicate neurons is targeted into...
Opiate receptor down-regulation in neuroblastoma X glioma NG108-15 hybrid cells possibly involved the internalization of ligand-receptor complexes during chronic treatment. However, was not supported by observed decrease [3H] enkephalin(D-Ala2,D-Leu5) ( [3H]DADLE) associated with prolonged incubation 10 nM [3H]DADLE at 37 degrees C. This bound determined to be due degradation complexes, for a time-dependent increase when incubations were carried out presence 0.1 mM chloroquine. The did...
Opioids classically regulate the excitability of neurons by suppressing synaptic GABA release from inhibitory neurons. Here, we report a role for opioids in modulating excitatory transmission. By activating ubiquitously clustered μ-opioid receptor (MOR) synapses, morphine caused collapse preexisting dendritic spines and decreased α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors. Meanwhile, opioid antagonist naloxone increased density spines. Chronic treatment with even presence...