A5047214928- Acute Myeloid Leukemia Research
- Hematopoietic Stem Cell Transplantation
- Chronic Myeloid Leukemia Treatments
- Immune Cell Function and Interaction
- Acute Lymphoblastic Leukemia research
- MicroRNA in disease regulation
- Chronic Lymphocytic Leukemia Research
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- RNA Interference and Gene Delivery
- T-cell and B-cell Immunology
- Mesenchymal stem cell research
- Erythrocyte Function and Pathophysiology
- Protein Degradation and Inhibitors
- Cancer-related molecular mechanisms research
- Pluripotent Stem Cells Research
- Monoclonal and Polyclonal Antibodies Research
- Virus-based gene therapy research
- RNA modifications and cancer
- CRISPR and Genetic Engineering
- Glycosylation and Glycoproteins Research
- Immune Response and Inflammation
- Biomedical Ethics and Regulation
- Childhood Cancer Survivors' Quality of Life
- Hematological disorders and diagnostics
University of Maryland, Baltimore
2015-2024
Sidney Kimmel Comprehensive Cancer Center
2001-2023
University of Maryland Marlene and Stewart Greenebaum Comprehensive Cancer Center
1984-2023
Pediatrics and Genetics
2004-2023
Institute for Stem Cell Biology and Regenerative Medicine
2011-2021
U-M Rogel Cancer Center
2012-2020
University of Baltimore
1985-2017
Johns Hopkins University
2000-2010
Johns Hopkins Medicine
1999-2010
University of Maryland, College Park
2010
MicroRNAs (miRNAs) are a recently identified class of epigenetic elements consisting small noncoding RNAs that bind to the 3′ untranslated region mRNAs and down-regulate their translation protein. miRNAs play critical roles in many different cellular processes including metabolism, apoptosis, differentiation, development. We found 33 expressed CD34+ hematopoietic stem-progenitor cells (HSPCs) from normal human bone marrow mobilized peripheral blood stem cell harvests. then combined these...
Abstract To facilitate the direct study of molecular events that control development human burst‐forming units‐erythroid (BFU‐E), we have developed a method to purify BFU‐E from peripheral blood. Using density centrifugation, rosetting with mixture neuraminidase‐treated and IgG‐coated sheep erythrocytes, positive panning anti‐My10 monoclonal antibody, overnight adherence plastic dishes, negative antibodies, blood were purified 0.04% 56.6%, 1,400‐fold purification 13% yield. More than 90%...
Nonadherent marrow mononuclear cells enriched for hematopoietic progenitor were cultured in semisolid medium with recombinant human granulocyte-macrophage colony-stimulating factor 9 days to form colony forming unitgranulocyte macrophage (CFU-GM) colonies. 1,25-Dihydroxyvitamin D was then gently layered over the cultures. After 2 weeks, approximately 30% of colonies that formed composed a unique polygonal morphology. One hundred percent these crossreacted monoclonal antibody 23c6, which...
Morphological and biochemical studies of human colony-forming units-erythroid (CFU-E) have been hindered by their extreme rarity. Since burst-forming (BFU-E) develop into CFU-E, we used normal blood BFU-E to generate large numbers highly purified CFU-E in vitro. Using density centrifugation, sheep erythrocyte rosetting, surface immunoglobulin-positive cell depletion, adherence plastic, negative panning with monoclonal antibodies, were from 0.017 0.368%, a 22-fold purification 43% yield. The...
Notch signaling regulates cell fate decisions in a variety of adult and embryonic tissues, represents characteristic feature exocrine pancreatic cancer. In developing mouse pancreas, targeted inactivation pathway components has defined role for regulating early endocrine differentiation, but been less informative with respect to possible subsequent differentiation events. Here, we show that activated target genes actively repress completion an acinar program zebrafish pancreas. the gene Hes1...
Abstract Several members of the Kruppel-like factor (KLF) family transcription factors play important roles in differentiation, survival, and trafficking blood immune cell types. We demonstrate this study that hematopoietic cells from KLF4−/− fetal livers (FL) contained normal numbers functional progenitor cells, were radioprotective, performed as well KLF4+/+ competitive repopulation assays. However, “KLF4−/− chimeras” generated by transplantation into lethally irradiated wild-type mice...
Summary A series of 23 monoclonal antibodies reactive with normal lymphoid and myeloid cells at various stages differentiation were used to characterize 96 adult patients acute myelocytic leukaemia (AML), concentrating on the possible role expression these antigens may have in predicting response intensive chemotherapy. Only CD34 ( P = 0·008) HLA‐DR 0·035) was significant therapy; leukaemic expressing (My10) had a complete remission (CR) rate 59% compared 87% for those blasts not antigen. In...
We used a recently developed strategy to analyze patterns of X-chromosome inactivation in human cell populations order study female members families with X-linked agammaglobulinemia — i.e., detect the carrier state and test hypothesis that disorder results from defect intrinsic development B cells. According this strategy, recombinant-DNA probes simultaneously restriction-fragment–length polymorphisms methylation genes. Random X-inactivation were observed isolated peripheral-blood...
PURPOSE The objective of this study was to characterize CD34+ cell grafts, obtained using a novel technique, from children undergoing autologous bone marrow transplantation (BMT) for cancer therapy. In particular, we wanted determine if the grafts generated hematopoietic reconstitution, since positive result would motivate further development and use methodology. PATIENTS AND METHODS This pilot feasibility clinical trial involved 13 patients < or = 25 years age with advanced solid tumors,...
Lentiviral vectors (LVs) offer several advantages over traditional oncoretroviral vectors. LVs efficiently transduce slowly dividing cells, including hematopoietic stem-progenitor cells (HSCs), resulting in stable gene transfer and expression. Additionally, recently developed self-inactivating (SIN) allow promoter-specific transgene For many applications, transduction of more than one is needed. We obtained inconsistent results our attempts to coexpress two transgenes linked by an internal...
Abstract Highly purified human erythroid colony‐forming cells (ECFC), which consist predominately of units‐erythroid (CFU‐F), were prepared from blood and used to study the binding processing erythropoietin (Ep). When radioiodinated recombinant Ep ( 125 I‐rEp) was incubated with these cells, specific saturable. Specific directly proportional cell concentration did not occur other cells. Saturation at 3°C occurred 1 nM (3.9/U/ml), Scatchard analysis revealed two classes sites on surface. Of a...
The human CD34(+)/CD38(-)/Lin(-) cell subset, comprising approximately 1-10% of the CD34(+) population, contains few less primitive hematopoietic (lineage-committed) progenitor cells (HPCs) but most in vivo engrafting (lympho-)hematopoietic stem (HSCs). We analyzed gene expression populations isolated from normal adult donor bone marrow, neonatal placental/umbilical cord blood, and mobilized peripheral blood stem-progenitor cells. As measured by Affymetrix microarrays, 4746 genes were...
In this paper, we report analysis of differentiation in human hemopoietic colonies derived from a single cell. Cord blood mononulear cells and panned My-10 antigen-positive bone marrow cord were plated methylcellulose medium containing erythropoietin conditioned medium. Initially, performed mapping studies to identify candidate colony-forming cells. Subsequently, using micromanipulator, transferred individually 35-mm dishes for colony formation. Cellular composition the was determined by...