A5060717412- Bone Metabolism and Diseases
- Bone health and treatments
- Bone health and osteoporosis research
- Metabolism, Diabetes, and Cancer
- Growth Hormone and Insulin-like Growth Factors
- Pain Mechanisms and Treatments
- Osteoarthritis Treatment and Mechanisms
- Neuroscience and Neuropharmacology Research
- Neuropeptides and Animal Physiology
- Ion channel regulation and function
- Bone fractures and treatments
- Bone and Dental Protein Studies
- Adipose Tissue and Metabolism
- TGF-β signaling in diseases
- Bone and Joint Diseases
- Inflammatory mediators and NSAID effects
- Diabetes Treatment and Management
- Axon Guidance and Neuronal Signaling
- Stress Responses and Cortisol
- Oral microbiology and periodontitis research
- Parathyroid Disorders and Treatments
- Estrogen and related hormone effects
- Fibroblast Growth Factor Research
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Molecular Sensors and Ion Detection
Royal Veterinary College
2015-2024
The University of Adelaide
2023
University College London
2015
University of London
2015
Universidad de Londres
2012-2015
Université Claude Bernard Lyon 1
2005-2014
Inserm
1998-2012
Queen Mary University of London
2012
Hôpital Larrey
2009
Hôpital Edouard Herriot
1989-2006
Interleukin-1 (IL-1) alpha and beta tumor necrosis factor (TNF) are potent stimulators of bone resorption in vitro vivo. However, the mechanisms underlying this increased have not been clearly defined. Increased can result from activity individual osteoclasts, numbers or both. Therefore, we used a long-term human marrow culture system that forms multinucleated cells (MNC) with characteristics osteoclasts to examine effects IL-1 TNF on osteoclast formation. Human recombinant beta, TNF-alpha...
Transforming growth factor beta (TGF-beta), a polypeptide present in abundant amounts bone matrix, was examined for its effects on osteoclast formation by using human marrow culture system which multinucleated cells (MNCs) with characteristics form. TGF-beta strongly inhibited MNC at concentrations as low 1 ng/ml. also completely suppressed the of osteotropic factors known to stimulate formation. The inhibitory effect osteoclast-like cell more pronounced during first week culture,...
Nonadherent marrow mononuclear cells enriched for hematopoietic progenitor were cultured in semisolid medium with recombinant human granulocyte-macrophage colony-stimulating factor 9 days to form colony forming unitgranulocyte macrophage (CFU-GM) colonies. 1,25-Dihydroxyvitamin D was then gently layered over the cultures. After 2 weeks, approximately 30% of colonies that formed composed a unique polygonal morphology. One hundred percent these crossreacted monoclonal antibody 23c6, which...
Osteocalcin, also called Bone Gla Protein (BGP), is the most abundant of non-collagenous proteins bone produced by osteoblasts. It consists a single chain 46-50 amino acids, according to species, and contains three vitamin K-dependent gamma-carboxyglutamic acid residues (GLA), involved in its binding calcium hydroxylapatite. Accumulating evidences suggest involvement remodeling, physiological role, however, still unclear. In this study adhesion properties biological effects osteocalcin on...
The present study shows no adverse effects of the anti-diabetic drug metformin on bone mass and fracture healing in rodents but demonstrates that is not osteogenic vivo, as previously proposed. In view increased incidence fractures patients with type 2 diabetes mellitus (T2DM), we investigated metformin, a widely used T2DM therapy, vivo using two different rodent models modes administration. We first subjected 12-week-old female C57BL/6 mice to ovariectomy (OVX). Four weeks after OVX,...
Abstract Bone tissue is densely innervated, and there increasing evidence for a neural control of bone metabolism. Semaphorin‐3A very important regulator neuronal targeting in the peripheral nervous system as well angiogenesis, knockout gene induces abnormal cartilage development. We analyzed spatial temporal expression patterns signaling molecules during endochondral ossification, parallel with establishment innervation. show that osteoblasts chondrocytes differentiated vitro express most...
Although Paget's disease is the most flagrant example of a primary osteoclast disorder, little known biology in this disease. In report we have studied formation cells with phenotype long-term cultures marrow mononuclear derived from patients disease, and compared these similar formed normal individuals, osteoclasts present pagetic bone. Osteoclasts resembled bone, but were distinctly different cultures. Osteoclast was 10-20-fold greater than The multinucleated much larger size,...
Primary bone cell cultures were derived from human explants. Cellular activity was characterized by the alkaline phosphatase (AP) activity, osteocalcin, and type I III collagen secretions in supernatant. The determination of performed three different wells. No significant difference noted between wells: coefficient variation 8.0 +/- 2.9% for AP 18.3 1.9% osteocalcin secretion, 22.5 14.3% collagen. secretion significantly decreased with number passages: they highest after first passage....
Recent studies have demonstrated that bone is highly innervated and contains neuromediators functional receptors on cells. However, no data exist concerning the quantitative changes of innervation during loss associated with estrogen withdrawal. To study involvement nerve fibers in regulation remodeling, we evaluated modifications a classical vivo model osteopenia rats, ovariectomy (OVX). Skeletal was studied by immunocytochemistry using antibodies directed against specific neuronal markers,...
Abstract The contribution of the SNS to bone's response mechanical loading is unclear. Using a noninvasive model axial murine tibia, we found that sciatic neurectomy enhances load-induced new cortical bone formation and pharmacological blockade does not affect such responses, indicating mediate osteogenic effects in bone. Introduction: There increasing evidence sympathetic nervous system (SNS) contributes regulation mass may influence remodeling by modulating bones' load-bearing. aim this...
Abstract Although the responses of bone to increased loading or exercise have been studied in detail, our understanding effects decreased usage skeleton has limited by scarcity suitable models. Such models should ideally not affect innervation, which appears be a mediator physiological unloading. MyoD–/–/Myf5–/– (dd/ff) mice lack skeletal muscle, so fetuses develop without any active movement utero and die soon after birth. We used micro‐compter tomography histology analyse their development...
Bone sialoprotein (BSP) is a protein highly specific for bone, which contains an arginine-glycine-aspartic acid (RGD) cell attachment sequence involved in osteoclast adhesion to bone matrix via the vitronectin receptor. We have investigated its role vitro resorption using well described isolated pit assay. BSP significantly stimulates dose-dependent manner, increasing overall resorbed area on ivory slices and number of lacunae at concentrations as low 50 nM. Neither recombinant osteopontin...
Abstract We previously identified functional N ‐methyl‐ d ‐aspartate (NMDA) glutamate receptors in mature osteoclasts and demonstrated that they are involved bone resorption vitro. In the present work, we studied expression of NMDA (NMDAR) by osteoclast precursors their role osteoclastogenesis using two vitro models, murine myelomonocytic RAW 264.7 cell line mouse marrow cells, both which differentiate into presence macrophage colony‐stimulating factor (M‐CSF) Rank ligand (RankL). Using...