A5031424240- Bone health and osteoporosis research
- Bone Metabolism and Diseases
- Bone health and treatments
- Bone fractures and treatments
- Sports Performance and Training
- Body Composition Measurement Techniques
- Sports injuries and prevention
- Metabolism, Diabetes, and Cancer
- Osteoarthritis Treatment and Mechanisms
- Estrogen and related hormone effects
- Parathyroid Disorders and Treatments
- Genetics and Physical Performance
- Muscle Physiology and Disorders
- Trauma and Emergency Care Studies
- HER2/EGFR in Cancer Research
- Growth Hormone and Insulin-like Growth Factors
- Lower Extremity Biomechanics and Pathologies
- COVID-19 and healthcare impacts
- Wnt/β-catenin signaling in development and cancer
- Knee injuries and reconstruction techniques
- TGF-β signaling in diseases
- Magnetic Properties and Synthesis of Ferrites
- Polydiacetylene-based materials and applications
- Delphi Technique in Research
- Occupational Health and Performance
Melbourne Health
2021
The University of Melbourne
2021
University College London
2019
Royal Veterinary College
2007-2016
Royal College of Physicians
2013
University of London
2008-2011
Indiana University – Purdue University Indianapolis
2004-2007
Indiana University School of Medicine
2004-2006
Indiana University Bloomington
2005
Deakin University
2003
The Wnt/beta-catenin pathway has been implicated in bone cell response to their mechanical environment. This is the origin of mechanism by which cells adjust architecture maintain strength. Osteoporosis most widespread failure this mechanism. degree osteoporotic loss men and women related bio-available estrogen. Here we report that osteoblastic ROS 17/2.8 primary osteoblast cultures, a single short period dynamic strain, as well glycogen synthase kinase-3beta (GSK-3beta) inhibitor LiCl,...
Abstract Estrogen receptor-α (ERα) is crucial for the adaptive response of bone to loading but role endogenous estradiol (E2) this unclear. To determine in vivo ligand dependency and relative roles different ERα domains osteogenic mechanical loading, gene-targeted mouse models with (1) a complete inactivation (ERα−/−), (2) specific activation function 1 (AF-1) (ERαAF-10), or (3) ERαAF-2 (ERαAF-20) were subjected axial tibia, presence absence (ovariectomy [ovx]) E2. Loading increased cortical...
To investigate the role of low-density lipoprotein receptor-related protein 5 (Lrp5) in bones' responses to loading, we analysed changes multiple measures bone architecture tibias subjected loading or disuse male and female mice with Lrp5 loss function mutation (Lrp5(-/-)) heterozygous for G171V High Bone Mass (HBM) (Lrp5(HBM+)).The right these 17week old their Wild Type (WT) littermates were short periods three days a week two weeks. Each tibia was loaded 40 cycles, produce peak strains at...
ObjectivesChanges in subchondral bone (SCB) and cross-talk with articular cartilage (AC) have been linked to osteoarthritis (OA). Using micro-computed tomography (micro-CT) this study: (1) examines changes SCB architecture a non-invasive loading mouse model which focal AC lesions are induced selectively the lateral femur, (2) determines any modifications contralateral knee, gait, might complicate use of limb as an internal control.MethodsRight knee joints CBA mice were loaded: once 2weeks...
Loading-related changes in gene expression resident cells the tibia of female mice contexts normality (WT), estrogen deficiency (WT-OVX), absence receptor alpha (ERalpha(-/-)) and disuse due to sciatic neurectomy (WT-SN) were established by microarray. Total RNA was extracted from loaded contra-lateral non-loaded tibiae at selected time points after a single, short period dynamic loading sufficient engender an osteogenic response. There marked many genes according context as well response...
Abstract Pre- and early puberty may be the most opportune time to strengthen female skeleton, but there are few longitudinal data support this claim. Competitive premenarcheal (pre/peri, n = 13) postmenarcheal (post, 32) tennis players aged 10 17 years were followed over 12 months. The osteogenic response loading was studied by comparing playing nonplaying humeri for dual-energy X-ray absorptiometry (DXA) bone mineral content (BMC) magnetic resonance imaging (MRI) total area (ToA), medullary...
Bones' functionally adaptive responses to mechanical loading can usefully be studied in the tibia by application of loads between knee and ankle normal genetically modified mice. Such also deforms fibula. Our present study was designed ascertain whether fibula adapts a similar way could thus provide an additional bone which functional adaptation. The right tibiae/fibulae C57BL/6 mice were subjected single period axial (40 cycles at 10 Hz with 10-second intervals each cycle; approximately 7...
To determine the effect of estrogen receptors (ER) α and β on bones' adaptive response to loading, we subjected right tibiae mice lacking ERα or ERβ activity either axial loading disuse. Adaptive changes in architecture were assessed by comparing differences between (treated) left (control) these genotypes as microcomputed tomography. In female ERα−/− mice, net-osteogenic was lower cortical bone compared with their wild-type littermates (11.2 vs. 20.9% ERα+/+), but it higher both cancellous...
Mechanical loading caused by physical activity can stimulate bone formation and strengthen the skeleton. Estrogen receptors (ERs) play some role in signaling cascade that is initiated cells after a mechanical load applied. We hypothesized one of ERs, ER-beta, influences responsiveness to loads. To test our hypothesis, 16-wk-old male female mice with null mutations ER-beta (ER-beta(-/-)) had their right forelimbs subjected short daily bouts. The technique used has been shown increase ulna....
Of the 1,328 genes revealed by microarray to be differentially regulated disuse, or at 8 h following a single short period of osteogenic loading mouse tibia, analysis predicting associated transcription factors from annotated affinities factor EGR2/Krox-20 as being more closely with pathways and functions than any other. Real time quantitative PCR confirmed up-regulation Egr2 mRNA expression tibia in vivo. In vitro studies where strain was applied primary cultures tibia-derived osteoblastic...
Acute management of people who have had a myocardial infarction (MI) has changed dramatically, with more surviving. More effective treatment, including percutaneous coronary intervention, raises questions about the relevance current recommendations on secondary prevention. Concerns arisen need for antiplatelet regimens mandated by stent insertion in an independent warfarin. New data are also available how to increase attendance cardiac rehabilitation programmes, existing drug treatments and...
Women are at greater risk of tearing their knee anterior cruciate ligament (ACL) than men participating in similar athletic activities. There is currently no conclusive explanation for this disparity; however, as ACL injuries women have been linked with estrogen fluctuations during the menstrual cycle, one hypothesis that has a direct detrimental effect on mechanical properties. This study investigated influence and its receptors (ERα ERβ) was achieved by testing viscoelastic tensile...
Although Lrp5 is known to be an important contributor the mechanisms regulating bone mass, its precise role remains unclear. The aim of this study was establish whether mutations in are associated with differences growth and/or apoptosis osteoblast-like cells and their proliferative response mechanical strain vitro. Primary were derived from cortical adult mice lacking functional (Lrp5−/−), those heterozygous for human G171V High Bone Mass (HBM) mutation (LRP5G171V) WT littermates (WTLrp5,...
Low circulating IGF-I is associated with increased fracture risk. Conditional depletion of produced in osteoblasts or osteocytes inhibits the bone anabolic effect mechanical loading. Here, we determined role endocrine for osteogenic response to loading young adult and old female mice adult, liver-specific inactivation (LI-IGF-I(-/-) mice, serum reduced by ≈70%) control mice. The right tibia was subjected short periods axial cyclic compressive three times/wk 2 wk, measurements were performed...
The COVID-19 pandemic has had a profound effect on the presentation and management of trauma at Royal Melbourne Hospital, level 1 adult major service designated hospital. This study compares changes in epidemiology patient access to emergency imaging surgery during response.The population interest was all patients captured hospital's registry from 16 March 2016 10 September 2020. Regression modelling assessed mechanism severity injury, mortality two lockdowns compared with proceeding 4...
Objectives The threat of a pandemic, over and above the disease itself, may have significant broad effects on healthcare system. We aimed to describe impact SARS-CoV-2 pandemic (during relatively low transmission period) associated societal restrictions presentations, admissions outpatient visits. Design compared hospital activity in 2020 with preceding 5 years, 2015–2019, using retrospective cohort study design. Setting Quaternary Melbourne, Australia. Participants Emergency department...