A5072483254- Bone Metabolism and Diseases
- Bone health and treatments
- Adipose Tissue and Metabolism
- Cell Adhesion Molecules Research
- Multiple Myeloma Research and Treatments
- Bone and Dental Protein Studies
- Exercise and Physiological Responses
- Bone health and osteoporosis research
- Adipokines, Inflammation, and Metabolic Diseases
- Eicosanoids and Hypertension Pharmacology
- Mesenchymal stem cell research
- Mitochondrial Function and Pathology
- dental development and anomalies
- Oral microbiology and periodontitis research
- Cell death mechanisms and regulation
- Bone Tissue Engineering Materials
- Neuroendocrine regulation and behavior
- Spaceflight effects on biology
- Muscle metabolism and nutrition
- Protease and Inhibitor Mechanisms
- Genetic Syndromes and Imprinting
- Connective tissue disorders research
- Cancer, Hypoxia, and Metabolism
- Inflammatory mediators and NSAID effects
- Bone Tumor Diagnosis and Treatments
University of Bari Aldo Moro
2016-2025
Azienda Ospedaliera S.Giuseppe Moscati
2018-2024
John Wiley & Sons (United States)
2024
Hudson Institute
2024
Vita-Salute San Raffaele University
1994-2023
Imperial College London
2012
University of Parma
2005
University of Foggia
2004
University of L'Aquila
1998
Istituto Ortopedico Rizzoli
1994-1996
Significance Although exercise is a well known and potent stimulus for new bone formation, weightlessness or muscle loss characteristically cause loss, it has remained unclear how talks to bone, despite their close proximity. Here, we show that molecule irisin derived from skeletal in response profound effects enhancing mass improving the geometry strength specifically of cortical key function which resist bending torsion. Trabecular reservoir bodily calcium, remarkably spared. Irisin may...
We have investigated the nature of immediate cell signals produced by occupancy chicken osteoclast alpha v beta 3 integrin. Synthetic osteopontin and peptides from bone sialoprotein sequences containing Arg-Gly-Asp stimulated reductions in cytosolic Ca2+. The changes Ca2+ required sequence were blocked a monoclonal antibody to integrin, LM609. Osteoclast stimulation proteins through integrin did not require immobilization since soluble inhibited binding particles resorption. decrease related...
Abstract We previously showed that Irisin, a myokine released from skeletal muscle after physical exercise, plays central role in the control of bone mass. Here we report treatment with recombinant Irisin prevented loss hind-limb suspended mice when administered during suspension (preventive protocol) and induced recovery mass were injected due to period 4 weeks (curative protocol). MicroCT analysis femurs r-Irisin preserved both cortical trabecular mineral density, dramatic decrease volume...
It has been recently demonstrated that exercise activity increases the expression of myokine Irisin in skeletal muscle, which is able to drive transition white brown adipocytes, likely following a phenomenon transdifferentiation. This new evidence supports idea muscle can be considered an endocrine organ, given its ability target adipose tissue by promoting energy expenditure. In accordance with these findings, we hypothesized directly involved bone metabolism, demonstrating increase...
We report that oxytocin (OT), a primitive neurohypophyseal hormone, hitherto thought solely to modulate lactation and social bonding, is direct regulator of bone mass. Deletion OT or the receptor (Oxtr) in male female mice causes osteoporosis resulting from reduced formation. Consistent with low formation, stimulates differentiation osteoblasts mineralizing phenotype by causing up-regulation BMP-2, which turn controls Schnurri-2 3, Osterix, ATF-4 expression. In contrast, has dual effects on...
Hepatocyte growth factor (HGF), also known as scatter factor, is a powerful motogen, mitogen, and morphogen produced by cells of mesodermal origin, acting on epithelial endothelial cells. Its receptor the tyrosine kinase encoded c-MET protooncogene. We show that HGF expressed human primary osteoclasts, osteoclast-like cell lines, osteoblasts. In both lineages, stimulation triggers activity autophosphorylation. activation followed increase in intracellular Ca2+ concentration pp60c-Src kinase....
The mechanisms of Ca2+ entry and their effects on cell function were investigated in cultured chicken osteoclasts putative produced by fusion mononuclear precursors. Voltage-gated channels (VGCC) detected the membrane depolarization with K+, BAY K 8644, dihydropyridine antagonists. K+ dose-dependent increases cytosolic calcium ([Ca2+]i) glass coverslips. Half-maximal achieved at 70 mM K+. completely inhibited derivative channel blocking agents. 8644 (5 X 10(-6) M), a VGCC agonist, stimulated...
Osteocalcin, also called Bone Gla Protein (BGP), is the most abundant of non-collagenous proteins bone produced by osteoblasts. It consists a single chain 46-50 amino acids, according to species, and contains three vitamin K-dependent gamma-carboxyglutamic acid residues (GLA), involved in its binding calcium hydroxylapatite. Accumulating evidences suggest involvement remodeling, physiological role, however, still unclear. In this study adhesion properties biological effects osteocalcin on...
Significance In this study, we show that a primitive brain hormone, arginine vasopressin (AVP), which has hitherto been implicated in regulating water balance mammals, function skeletal homeostasis. Using genetically modified mice are lacking one of the AVP receptors as well pharmacologic inhibitors, negatively regulates osteoblasts (cells form new bone) and stimulates osteoclasts remove old bone). Our findings explain bone loss is known to accompany low blood sodium levels patients when high.
Wingless-type (Wnt) signaling through the secretion of Wnt inhibitors Dickkopf1, soluble frizzled-related protein-2 and -3 has a key role in decreased osteoblast (OB) activity associated with multiple myeloma (MM) bone disease. We provide evidence that another antagonist, sclerostin, an osteocyte-expressed negative regulator formation, is expressed by cells, is, human cell lines (HMCLs) plasma cells (CD138+ cells) obtained from marrow (BM) large number MM patients demonstrated BM stromal...
ABSTRACT Previous results showed that intermittently administered irisin improves bone mass in normal mice and prevents the development of disuse-induced osteoporosis muscular atrophy hindlimb-suspended mice, a murine model able to mimic absence mechanical loading. A recent study increases survival osteocytes acting through integrin αV/β5 receptors. To better understand action on these cells, we investigated downstream signaling cascades osteocyte-like cells (MLO-Y4) treated with recombinant...
The covalent attachment of an Arg-Gly-Asp (RGD) containing peptide to polypyrrole(PPy)-coated titanium substrates has been investigated in order develop a bioactive material potential use orthopedic fields. Polypyrrole employed as the coating polymer because its suitability be electrochemically grown directly onto metallic different shapes, leading remarkably adherent films. synthetic Cys-Gly-(Arg-Gly-Asp)-Ser-Pro-Lys, cell-adhesive region fibronectin (RGD), grafted substrate via cysteine...
Increased plasma homocysteine (HCY) may be an independent risk factor for osteoporotic fractures and therefore also adversely affect bone metabolism. We analyzed the effect of HCY on human osteoclast (OC) activity.We cultured peripheral blood mononuclear cells from 17 healthy male donors [median (SD) age, 30 (5) years] 20 days with 25 microg/L macrophage-colony-stimulating (days 0-11), receptor-activator nuclear factor-kappaB ligand 6-20), 4 different concentrations (0, 10, 50, 100...
During space flight, severe losses of bone mass are observed. Both formation and resorption probably involved, but their relative importance remains unclear. The purpose this research is to understand the role osteoclasts precursors in microgravity-induced loss. Three experiments on isolated (OCs) precursors, OSTEO, OCLAST, PITS, were launched FOTON-M3 mission. OSTEO experiment was conducted for 10 d microgravity within bioreactors with a perfusion system, where differentiation cultured...
Dental pulp stem cells (DPSCs) are an adult cell population with high proliferative potential and the ability to differentiate in many types, this has led scientists consider these be alternative source of postnatal comparable mesenchymal from bone marrow. In work, we studied osteoblastic phenotype developed by DPSCs cultured osteogenic medium. particular, analyzed expression typical osteoblast markers such as alkaline phosphatase, collagen type I, osteocalcin, osteopontin, well mineralized...
Sclerostin, an osteocyte‐expressed negative regulator of bone formation, is one the inhibitors Wnt signaling that a critical pathway in correct process osteoblast differentiation. It has been demonstrated through secretion inhibitors, such as DKK1, sFRP‐2, and sFRP‐3, plays key role decreased activity associated with multiple myeloma (MM) disease. We provide evidence sclerostin expressed by cells are human cell lines plasma (CD138 + cells) obtained from marrow (BM) large number MM patients...
Childhood type 1 diabetes mellitus (T1DM) is associated with decreased bone mass. Sclerostin and dickkopf-1 (DKK-1) are Wnt inhibitors that regulate formation.To evaluate sclerostin DKK-1 levels in T1DM children to analyze the influence of glycemic control on health.Cross-sectional study conducted at a clinical research center.One hundred six subjects (12.2 ± 4 years), 66 multiple daily injections (MDIs) 40 continuous subcutaneous infusion insulin (CSII), 80 controls.The average transmission...
Several studies have reported the beneficial effects of mesenchymal stem cells (MSCs) in tissue repair and regeneration. New sources adult organisms are continuously emerging; dental tissues been identified as a source postnatal MSCs. Dental bud is immature precursor tooth, easy to access we show this study that it can yield high number with ≥95% expression stemness makers osteogenic capacity. Thus, these be defined Bud Stem Cells (DBSCs) representing promising for bone regeneration...