A5050412467- Orthopedic Infections and Treatments
- Advanced X-ray and CT Imaging
- Spectroscopy Techniques in Biomedical and Chemical Research
- Molecular Biology Techniques and Applications
- Connective tissue disorders research
- Extracellular vesicles in disease
- Congenital heart defects research
- Manufacturing Process and Optimization
- Mesenchymal stem cell research
- Neuroendocrine regulation and behavior
- Bone fractures and treatments
- Psoriasis: Treatment and Pathogenesis
- Electrolyte and hormonal disorders
- Immunodeficiency and Autoimmune Disorders
- Anesthesia and Neurotoxicity Research
- MicroRNA in disease regulation
- Bone and Dental Protein Studies
- Tissue Engineering and Regenerative Medicine
- Protein Tyrosine Phosphatases
- Cytokine Signaling Pathways and Interactions
- Advanced Measurement and Metrology Techniques
- Renal Diseases and Glomerulopathies
- Cardiovascular Issues in Pregnancy
- Systemic Lupus Erythematosus Research
- Mycobacterium research and diagnosis
University College Hospital
2024-2025
University College London
2016-2025
London Women's Clinic
2016-2024
St George's, University of London
2022
Institute of Child Health
2018
Université Paris Cité
2016
Hôpital Lariboisière
2016
Inserm
2016
University of Bari Aldo Moro
2010-2013
University of Dundee
2010
Significance In this study, we show that a primitive brain hormone, arginine vasopressin (AVP), which has hitherto been implicated in regulating water balance mammals, function skeletal homeostasis. Using genetically modified mice are lacking one of the AVP receptors as well pharmacologic inhibitors, negatively regulates osteoblasts (cells form new bone) and stimulates osteoclasts remove old bone). Our findings explain bone loss is known to accompany low blood sodium levels patients when high.
Human mesenchymal stem cells (MSCs) have huge potential for regenerative medicine. In particular, the use of pluripotent cell-derived (PSC-MSCs) overcomes hurdle replicative senescence associated with in vitro expansion primary and has increased therapeutic benefits comparison to various adult sources MSCs a wide range animal disease models. On other hand, fetal exhibit faster growth kinetics possess longer telomeres wider differentiation than MSCs. Here, first time, we compare PSC-MSCs...
In Europe, approximately 85–90% of individuals with Osteogenesis Imperfecta (OI) have dominant pathogenic variants in the Col1a1 or Col1a2 genes whilst for Asian, especially Indian and Chinese cohorts, this ratio is much lower. This leads to decreased abnormal Collagen type I production. Subsequently, bone formation strongly reduced, causing fragility liability fractures throughout life. OI clinically heterogeneous, severity ranging from mild lethal depending on gene location OI-causative...
Human fetal mesenchymal stem cells (hfMSCs) present advantageous characteristics compared to their adult counterparts and have emerged as potent in the field of regenerative medicine. In context skeletal regeneration, human amniotic fluid (AFSCs) been shown improve quality structure bone extracellular matrix an experimental model severe osteogenesis imperfecta. However, primary hfMSCs undergo replicative senescence during vitro expansion, along with a progressive decrease plasticity tissue...
Abstract The impaired maturation of bone-forming osteoblasts results in reduced bone formation and subsequent weakening, which leads to a number conditions such as osteogenesis imperfecta (OI). Transplantation human fetal mesenchymal stem cells has been proposed skeletal anabolic therapy enhance formation, but the mechanisms underlying contribution donor health are poorly understood require further elucidation. Here, we show that intraperitoneal injection amniotic (AFSCs) into mouse model OI...
Alport syndrome (AS) is a hereditary glomerulopathy caused by mutation in type IV collagen genes, which disrupts glomerular basement membrane, leading to progressive glomerulosclerosis and end-stage renal failure. There at present no cure for AS, cell-based therapies offer promise improve function. In this study, we found that human first trimester fetal chorionic stem cells (CSC) are able migrate glomeruli differentiate down the podocyte lineage vitro vivo. When transplanted into 7-week-old...
Human amniotic fluid contains two morphologically-distinct sub-populations of stem cells with regenerative potential, spindle-shaped (SS-hAFSCs) and round-shaped human (RS-hAFSCs). However, it is unclear whether morphological differences correlate functionality, this lack knowledge limits their translational applications. Here, we show that SS-hAFSCs RS-hAFSCs differ in neuro-protective ability, demonstrating a single contralateral injection into hypoxic-ischemic P7 mice conferred 47%...
Abstract Idiopathic scoliosis (IS) is the deformation and/or abnormal curvature of spine that develops progressively after birth. It a very common condition, affecting approximately 4% general population, yet genetic and mechanistic causes IS are poorly understood. Here, we focus on PPP2R3B , which encodes protein phosphatase 2A regulatory subunit. We found expressed at sites chondrogenesis within human foetuses, including vertebrae. also demonstrated prominent expression in myotome muscle...
Abstract Micro-computed tomography (micro-CT) is commonly used to assess bone quality and evaluate the outcome of experimental therapies in animal models diseases. Generating large datasets however challenging data are rarely made publicly available through shared repositories. Here we describe a dataset micro-CT reconstructed scans proximal part 21 tibiae from wild-type mice, osteogenesis imperfecta mice (homozygous oim/oim) oim/oim transplanted with human amniotic fluid stem cells. The...
Abstract Approximately 85-90% of individuals with Osteogenesis Imperfecta (OI) have dominant pathogenic variants in the COL1A1 or COL1A2 genes. This leads to decreased abnormal Collagen type I production. Subsequently, bone formation is strongly reduced, causing fragility and liability fractures throughout life. OI clinically classified 5 types severity ranging from mild lethal depending on gene location OI-causative variant subsequent effect (pro) collagen synthesis. However, specific...
Abstract Idiopathic scoliosis (IS) is the deformation and/or abnormal curvature of spine that develops progressively after birth. It a very common condition, affecting approximately 4% general population, yet genetic and mechanistic causes IS are poorly understood. Here, we focus on PPP2R3B , which encodes protein phosphatase 2A regulatory subunit has been linked to regulation autophagy. We found expressed at sites chondrogenesis within human foetuses, including vertebrae. also demonstrated...
To the Editor: We thank Yapici et al. (1) for their interest in our study (2). agree with them that choice of antibody is paramount importance a based on immunohistochemistry results. Our Santa Cruz present was existing literature (3-5) and previous work (6). In recent performed graft biopsies transplant recipients delayed function, we used same did not observe either any epithelial expression or colocalization CD4 within interstitial infiltrate Indeed, this particular set patients, IL-17...
Searchable abstracts of presentations at key conferences on calcified tissues ISSN 2052-1219 (online)
Introduction and Aims: Oxidative stress (OS) causing endothelial dysfunction (ED) is thought to be a major reason why renal patients suffer more cardiovascular (CV) events than would expected from their conventional risk factors such as BP.One way reduce OS prevent its formation by using allopurinol block xanthine oxidase (XO)-induced OS.In this study, we examined if really does improve ED in patients.Methods: A randomised, double-blind, placebo-controlled, parallel study was conducted 67...