A5100402623- Hepatitis B Virus Studies
- Lung Cancer Research Studies
- Lung Cancer Treatments and Mutations
- Metal-Catalyzed Oxygenation Mechanisms
- Hepatitis C virus research
- Lung Cancer Diagnosis and Treatment
- Immunotherapy and Immune Responses
- Metal complexes synthesis and properties
- vaccines and immunoinformatics approaches
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Free Radicals and Antioxidants
- Pleural and Pulmonary Diseases
- Drug Transport and Resistance Mechanisms
- Autophagy in Disease and Therapy
- Tracheal and airway disorders
- Neuroendocrine Tumor Research Advances
- Congenital Diaphragmatic Hernia Studies
- Metalloenzymes and iron-sulfur proteins
- Porphyrin and Phthalocyanine Chemistry
- CO2 Reduction Techniques and Catalysts
- Liver Disease Diagnosis and Treatment
- Medical Imaging and Pathology Studies
- Organ Transplantation Techniques and Outcomes
- Cell death mechanisms and regulation
- Berberine and alkaloids research
Academy of Medical Sciences
2023
Chinese Academy of Medical Sciences & Peking Union Medical College
2017-2023
Academia Sinica
2023
Peking Union Medical College Hospital
2017-2023
WuXi AppTec (China)
2017-2022
Jiangsu University
2004-2022
Affiliated Hospital of Jiangsu University
2004-2022
Southeast University
2019-2022
Children's Hospital of Fudan University
2022
Zhejiang Provincial People's Hospital
2020
An approach to calculating molecular electronic structures of active-site clusters in the presence protein environments has been developed. The cluster is treated by density functional theory. field, together with reaction field arising mainly from solvent, obtained a finite-difference solution Poisson−Boltzmann equation three dielectric regions, and then these are coupled calculation self-consistent iterative procedure. method applied compute redox potentials ferredoxin Anabaena 7120...
It is the first time that mRNA-LNPs have been used to express anti-HBsAg antibodies (G12-scFv, G12-scFv-Fc, and G12-IgG). G12-scFv-Fc- G12-IgG-encoding exerted a sustained effect on HBsAg serum clearance in adeno-associated virus (AAV)/HBV mouse model with persistent expression.
Density functional and electrostatic methods have been applied to calculate active site geometries the redox potential of manganese superoxide dismutase (MnSOD). The initial clusters were built up by including only first-shell side chain ligands then augmented second-shell ligands. density optimized Mn−ligand bond lengths for reduced complexes in general compared fairly well with protein crystallography data; however, large deviations calculated Mn−OH distances found oxidized clusters. Our...
Abstract Non‐small cell lung cancer (NSCLC) with wild‐type epidermal growth factor receptor (EGFR) is intrinsic resistance to EGFR‐tyrosine kinase inhibitors (TKIs), such as afatinib. Celastrol, a natural compound antitumor activity, was reported induce paraptosis in cells. In this study, EGFR‐TKI‐resistant NSCLC lines H23 (EGFR and KRAS mutation) H292 overexpression) were used test whether celastrol could overcome primary afatinib through induction. The synergistic effect of on survival...
Lymph node metastasis is a major prognostic factor in resected non-small cell lung cancer (NSCLC). However, 30-40 % rate of recurrence after performing complete resection node-negative patients suggests that their nodal staging suboptimal. We aimed to evaluate the molecular diagnosis and significance lymph micrometastasis with NSCLC. Primary tumor samples from 62 stage I-IIB NSCLC were screened for fragile histidine triad (FHIT) CDKN2A mRNA deletion using reverse transcriptase polymerase...
Although host T cell immune responses to hepatitis B virus (HBV) have been demonstrated important influences on the outcome of HBV infection, development epitope-based vaccine and therapy clinical evaluation specific function are currently hampered markedly by lack validated epitopes covering broad patients. This study aimed screen spanning overall HBsAg, HBeAg, HBx HBpol proteins presenting thirteen prevalent human leukocyte antigen (HLA)-A allotypes which gather a total gene frequency...
The discovery of novel tetrahydropyrrolo[1,2-
Hepatitis B virus (HBV) infection is a rare etiology of cryoglobulinemia, and its clinical characteristics, virological features treatment are poorly understood.This retrospective study enrolled 23 patients with HBV-related cryoglobulinemia from 497 at Peking Union Medical College Hospital between January 2015 February 2023. We analyzed the management cryoglobulinemia.The (13 males; median age 48 years) were all mixed serological HBsAg positive, while 15 exhibited HBV-DNA replication. The...
The conflicting protein crystallography data for the oxidized form (MMOHox) of methane monooxygenase present a dilemma regarding identity solvent-derived bridging ligands within active site: do they comprise diiron unit bridged by 1H2O and 1OH- as postulated Methylococcus capsulatus or 2OH- suggested Methylosinus trichosporium? Using models derived explicitly from M. trichosporium data, spin-unrestricted density functional methods have been used to study two structurally characterized forms...
Gefitinib, an EGFR-tyrosine kinase inhibitor, significantly improve prognosis in patients with advanced non-small cell lung cancer (NSCLC). The aim of this study was to evaluate the usefulness MUC1 and vascular endothelial growth factor (VEGF) mRNA expression peripheral blood as means predicting benefit from gefitinib therapy NSCLC patients.MUC1 VEGF expressions were detected 66 before (B0) 4 weeks after treatment (B4w) gefitinib, using real-time quantitative-PCR assay. Correlations between...
Using the density functional optimized active site geometries obtained in accompanying paper (Lovell, T.; Li, J.; Noodleman, L. Inorg. Chem. 2001, 40, 5251), a combined and electrostatics approach has been applied to further address attendant uncertainties protonation states of bridging ligands for MMOHox. The acidities (pKas) associated with H2O ligand Methylococcus capsulatus corresponding energetics each cluster interacting protein environment have evaluated. pKa calculations combination...
To evaluate proportion and predictors of poor response in infants appraise booster seroprotection, we surveyed 2047 Shanghai detected antibody to hepatitis B (HB) surface antigen (anti-HBs). Poor responders were randomized into 2 groups, given with 5 µg 10 vaccine (HepB), respectively. Proportion titer <10 mIU/mL 99 was 1.86% 15.14%, Multivariate logistic regression suggested male, aged 13 18 months, premature, administered HepB or mother positive for HB (HBsAg) HBe (HBeAg) would more...
Abstract Background Circulating long noncoding RNAs (lncRNAs) are considered a new class of biomarkers for the diagnosis and prognosis various malignancies. We aimed to identify circulating lncRNAs as non‐small cell lung cancer (NSCLC). Methods The expression 14 candidate was measured in matched ipsilateral normal tissues 20 patients with NSCLC using quantitative reverse‐transcription PCR. In plasma samples from training testing sets, significantly aberrantly expressed lncRNAs, TA73‐AS1...