A5010691634- Cancer-related molecular mechanisms research
- Clinical Nutrition and Gastroenterology
- Neuroinflammation and Neurodegeneration Mechanisms
- Diet and metabolism studies
- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Liver Disease Diagnosis and Treatment
- Gut microbiota and health
- PI3K/AKT/mTOR signaling in cancer
- Cytokine Signaling Pathways and Interactions
- RNA regulation and disease
- RNA modifications and cancer
- Protein Kinase Regulation and GTPase Signaling
- Metabolism and Genetic Disorders
- Photoreceptor and optogenetics research
- Immune cells in cancer
- Inflammatory mediators and NSAID effects
- Bacterial biofilms and quorum sensing
- Neurogenesis and neuroplasticity mechanisms
- Circular RNAs in diseases
- Receptor Mechanisms and Signaling
- Ubiquitin and proteasome pathways
- Peroxisome Proliferator-Activated Receptors
- Cancer Research and Treatments
- Vibrio bacteria research studies
Xinqiao Hospital
2016-2025
Army Medical University
2016-2025
Shanghai Jiao Tong University
2016-2025
XinHua Hospital
2017-2025
Shanghai Institute of Nutrition and Health
2021-2025
Fuwai Yunnan Cardiovascular Hospital
2025
Chongqing Medical University
2021-2024
Jiangsu Agri-animal Husbandry Vocational College
2023
First Affiliated Hospital of Henan University
2021-2022
Ruijin Hospital
2017-2022
Rationale: Microglia play a critical role in modulating cell death and neurobehavioral recovery response to brain injury either by direct cell-cell interaction or indirect secretion of trophic factors.Exosomes secreted from cells are well documented deliver bioactive molecules recipient modulate function.Here, we aimed identify whether M2 microglia exert neuroprotection after ischemic attack through an exosome-mediated interaction.Methods: microglia-derived exosomes were intravenously...
Cancer secreted exosomal miRNAs are emerging as mediators between tumor-stoma crosstalk. Here, we show epithelial ovarian cancer (EOC)-derived exosomes activated macrophages to a tumor-associated macrophage (TAM)-like phenotype with SOCS3/STAT3 pathway involvement, which could facilitate the progression of cancer. MiR-222-3p was enrichment in released from EOC cells and it be transferred macrophages. Overexpression miR-222-3p induced polarization M2 phenotype. Luciferase assay verified...
Metabolic reprogramming is critical for the polarization and function of tumor-associated macrophages (TAM) hepatocarcinogenesis, but how this occurs unknown. Here, we showed that receptor-interacting protein kinase 3 (RIPK3), a central factor in necroptosis, downregulated hepatocellular carcinoma (HCC)-associated macrophages, which correlated with tumorigenesis enhanced accumulation M2 TAMs. Mechanistically, RIPK3 deficiency TAMs reduced reactive oxygen species significantly inhibited...
Abstract The dynamic regulations of ubiquitination and deubiquitination play important roles in TGF-β–activated kinase 1 (TAK1)-mediated NF-κB activation, which regulates various physiological pathological events. We identified ubiquitin-specific protease (USP)19 as a negative regulator TNF-α– IL-1β–triggered activation by deubiquitinating TAK1. Overexpression USP19 but not its enzymatic inactive mutant inhibited transcription downstream genes, whereas deficiency had the opposite effects....
To study the mechanism by which epithelial ovarian cancer (EOC)-derived exosomes restore migration of endothelial cells that is suppressed TAM-derived exosomes.Exosomes were isolated from TAMs in ascites patients with EOC. The effect on expression cell miRNA was monitored PCR. mimics transfected to explore their effects. Microarray data and literature searches used predict target genes impact gene pathways, small interfering RNA these genes. We assays determine whether cell-derived...
Studies demonstrate that microRNA-126 plays a critical role in promoting angiogenesis. However, its effects on angiogenesis following ischemic stroke are unclear. Here, we explored the effect of microRNA-126-3p and microRNA-126-5p neurogenesis after brain ischemia. We demonstrated both microRNA (miRNA)-126-3p increased proliferation, migration, tube formation human umbilical vein endothelial cells (HUVECs) compared with scrambled miRNA control (p < 0.05). Transferring into mouse middle...
Macrophages are involved in demyelination many brain diseases. However, the role of macrophages recovery phase ischemic is unknown. The present study aims to explore injury and tissue repair following a 90-min transient middle cerebral artery occlusion mice.Clodronate liposomes were injected into mice deplete periphery macrophages. These subsequently underwent occlusion. F4/80(+) CD68(+) cells examined mouse spleen confirm macrophage depletion at 14 days after Modified neurological severity...
Background: Cancer cells undergoing invasion and metastasis possess a phenotype with attenuated glycolysis, but enhanced fatty acid oxidation (FAO). Calcium (Ca2+)-mediated signaling pathways are implicated in tumor metabolism regulation. Stromal-interaction molecule 1 (STIM1) triggered store-operated Ca2+ entry (SOCE) is the major route of influx for non-excitable including hepatocellular carcinoma (HCC) cells. However, whether how STIM1 regulates HCC via metabolic reprogramming unclear....
Matrix metalloproteinase 9 (MMP-9) plays a beneficial role in the delayed phase of middle cerebral artery occlusion (MCAO). However, mechanism is obscure. Here, we constructed hypoxia response element (HRE)-regulated MMP-9 to explore its effect on glial scars and neurogenesis ischemic stroke. Adult male Institute Cancer Research (ICR) mice underwent MCAO received stereotactic injection lentivirus carrying HRE-MMP-9 or normal saline (NS)/lentivirus-GFP 7 days after ischemia. We found that...
Epithelial ovarian cancer (EOC) patients frequently suffer from thrombocytosis, which leads to a poor prognosis. However, the mechanism underlying platelet regulation of biological behavior in EOC remains unclear. The associations between clinicopathological characteristics and thrombocytosis 171 were studied, preoperative was significantly associated with stage, metastasis scope, level CA125 overall survival. When SKOV3 cells cocultured microparticles (PMPs), expression molecules...
Clinical outcomes for advanced malignant melanoma (MM) are often poor due to tumor invasiveness, metastasis, recurrence, and multidrug resistance.We investigated whether apoptosis, cell cycle regulation, oxidative status, redox balance were altered by changes in the expression of long noncoding RNA, growth arrest-specific transcript 5 (GAS5), MM cells.Analysis clinical samples from patients showed that rate reduced GAS5 expression, relative adjacent noncancerous tissues, was significantly...
Gut microbiota and microbial factors regulate the pathogenesis of nonalcoholic fatty liver disease (NAFLD) in patients with obesity metabolic abnormalities, but little is known about their roles nonobese NAFLD. Expansion Escherichia associated NAFLD pathogenesis. We aimed to investigate pathogenic role fergusonii its products development NAFLD.We characterized intestinal microbiome signature a cohort healthy controls by 16S ribosomal RNA sequencing. The E was estimated rats after 16 weeks...
RNA cargo in exosomes, especially microRNAs (miRNAs), play an important role the chemotherapy drug resistance of human cancers. However, and mechanism exosomal miR-107 on multidrug gastric cancer cells was still not clear. In this study, we sought to explore whether could reverse drugs.We extracted exosomes from sensitive (SGC-7901, MGC-803) resistant (SGC-7901/5-FU) by ultracentrifugation isolated were identified using transmission electron microscopy (TEM) dynamic light scattering analysis...
Background Human beta-defensin-1 (hBD-1) has recently been considered as a candidate tumor suppressor in renal and prostate cancer. The aim of this study was to investigate the role hBD-1 progression oral squamous cell carcinoma (OSCC) its potential diagnostic/prognostic biomarker therapeutic target for OSCC. Methods HBD-1 expression tissues at different stages carcinogenesis, well OSCC lines examined. overexpressed HSC-3, UM1, SCC-9 SCC-25 cells subjected growth, apoptosis, migration...
Abstract In this study, we aimed to explore the mechanism of glutathione peroxidase 3 (GPX3) in growth malignant melanoma (MM) cells by hypoxia‐inducible factor‐1α (HIF1‐α) and HIF2‐α regulating metabolism through reactive oxygen species (ROS). The messenger RNA protein expression GPX3, HIF1‐α, tissues, cell lines were measured reverse transcription‐quantitative PCR Western blot analysis. A375 transfected with GPX3 overexpression plasmid, small interfering (siRNA) targeting or siRNA...
Age-dependent alterations in microglia behavior have been implicated neurodegeneration and CNS injuries. Here, we compared the transcriptional profiles of young versus aged during stroke recovery. CD45 intermediate CD11b + were FACS-isolated from brains (10-week-old) (18-month-old) male mice with sham operation or 14 days after distal middle cerebral artery occlusion subjected to RNA-sequencing analysis. Functional groups enriched are indicative upregulation cell movement, interactions,...
Background & Aims: Liver cancer stem cells (LCSCs) mediate therapeutic resistance and correlate with poor outcomes in patients hepatocellular carcinoma (HCC). Fibroblast growth factor (FGF)-19 is a crucial oncogenic driver gene HCC correlates prognosis. However, whether FGF19 signaling regulates the self-renewal of LCSCs unknown. Methods: were enriched by serum-free suspension. Self-renewal characterized sphere formation assay, clonogenicity sorafenib assay tumorigenic potential assays. Ca2+...
Elucidating the normal structure and distribution of cerebral vascular system is fundamental for understanding its function. However, studies on visualization whole-brain quantification vasculature with cellular resolution are limited. Here, we explored at level using newly developed CLARITY technique. Adult male C57BL/6J mice undergoing transient middle artery occlusion Tie2-RFP transgenic were used. Whole mouse brains extracted processing. Immunostaining was performed to label vessels....
// Qiao Zhang 1, * , Zhe Yang 2, Qiaoqiao Han Honggang Bai 1 Yanling Wang Xiaojia Yi 3 Zihan Lijuan Lu Jiang Xin Song 4 Yingmin Kuang 5 and Yuechun Zhu Department of Biochemistry Molecular Biology, School Basic Medical Sciences, Kunming University, Chenggong District, 650500, Yunnan Province, China 2 Pathology, The First Affiliated Hospital Wuhua 650032, Second 650101, Cancer Biotherapy Center, Third Xishan 650118, Organ Transplantation, These authors have contributed equally to this work...
Abstract Metastasis remains a significant challenge in the treatment of hepatocellular carcinoma (HCC). The role ribonuclease T2 (RNASET2) HCC is still uncertain, although it has been reported to have contradictory effects on some cancers. Here, demonstrated that RNASET2 knockout leads accumulation cholesterol, which turn promotes MET‐mediated metastasis. Mechanistically, absence hinders degradation RNA into uridine, thereby reducing conversion UTP. This reduction restrains glucuronate...
To investigate the prevalence of scoliosis and congenital heart disease (CHD) in same area to explore relationship between them according a joint school screening. All students aged 6-15 years 20 schools Jinghong City, Yunnan Province, China was screened for CHD. Scoliosis screening completed through Adam's forward bending test with scoliometer measurement, CHD auscultation combined portable echocardiography (ECHO). The gender, age, distribution ethnic groups, types CHD, angle trunk rotation...
ABSTRACT The development and severity of metabolic dysfunction-associated steatotic liver disease (MASLD) in children are closely related to alterations gut microbiota. This study aims investigate changes the microbiota signature microbial metabolites with MASLD. We collected fecal samples from adolescents aged 6–16 years, presence MASLD was diagnosed by ultrasound. performed 16S ribosomal DNA sequencing targeted metabolomics 36 25 subjects, consisting healthy controls, obesity, α-diversity...