A5100759492- 3D Printing in Biomedical Research
- Additive Manufacturing and 3D Printing Technologies
- Innovative Microfluidic and Catalytic Techniques Innovation
- Neuroinflammation and Neurodegeneration Mechanisms
- Microbial Natural Products and Biosynthesis
- Photoreceptor and optogenetics research
- Cancer Cells and Metastasis
- Optical Imaging and Spectroscopy Techniques
- Neurogenesis and neuroplasticity mechanisms
- Angiogenesis and VEGF in Cancer
- Immune cells in cancer
- Synthetic Organic Chemistry Methods
- Photoacoustic and Ultrasonic Imaging
- Job Satisfaction and Organizational Behavior
- Nonlinear Optical Materials Studies
- Tissue Engineering and Regenerative Medicine
- Inflammatory Biomarkers in Disease Prognosis
- Cell Image Analysis Techniques
- Microfluidic and Bio-sensing Technologies
- Neural dynamics and brain function
- Cancer Immunotherapy and Biomarkers
- Mesenchymal stem cell research
- Neuroscience and Neural Engineering
- Barrier Structure and Function Studies
- Drug Transport and Resistance Mechanisms
Shanghai Jiao Tong University
2017-2025
Guangdong University Of Finances and Economics
2024-2025
Guangdong University of Finance
2024-2025
Renji Hospital
2021-2025
Brigham and Women's Hospital
2020-2024
Harvard University
2020-2024
University of Finance and Economics
2024
Jiaxing University
2021-2024
China University of Geosciences
2023-2024
Jiangmen Central Hospital
2023
Rationale: Microglia play a critical role in modulating cell death and neurobehavioral recovery response to brain injury either by direct cell-cell interaction or indirect secretion of trophic factors.Exosomes secreted from cells are well documented deliver bioactive molecules recipient modulate function.Here, we aimed identify whether M2 microglia exert neuroprotection after ischemic attack through an exosome-mediated interaction.Methods: microglia-derived exosomes were intravenously...
Abstract Three-dimensional (3D) hydrogel printing enables production of volumetric architectures containing desired structures using programmed automation processes. Our study reports a unique method resolution enhancement purely relying on post-printing treatment constructs. By immersing 3D-printed patterned consisting hydrophilic polyionic polymer network in solution polyions the opposite net charge, shrinking can rapidly occur resulting various degrees reduced dimensions comparing to...
Recapitulation of complex tissues signifies a remarkable challenge and, to date, only few approaches have emerged that can efficiently reconstruct necessary gradients in 3D constructs. This is true even though mimicry these great importance establish the functionality engineered and devices. Here, composable-gradient Digital Light Processing (DLP)-based (bio)printing system developed, utilizing unprecedented integration microfluidic mixer for generation either continual or discrete desired...
Digital light processing bioprinting favors biofabrication of tissues with improved structural complexity. However, soft-tissue fabrication this method remains a challenge to balance the physical performances bioinks for high-fidelity and suitable microenvironments encapsulated cells thrive. Here, we propose molecular cleavage approach, where hyaluronic acid methacrylate (HAMA) is mixed gelatin methacryloyl achieve high-performance bioprinting, followed by selectively enzymatic digestion...
Ischemic stroke induced matrixmetallo-proteinase-9 (MMP-9) upregulation, which increased blood-brain barrier permeability. Studies demonstrated that mesenchymal stem cell therapy protected disruption from several cerebrovascular diseases. However, the underlying mechanism was largely unknown. We therefore hypothesized cells reduced destruction by inhibiting and it related to intercellular adhesion molecule-1 (ICAM-1).Adult ICR male mice (n = 118) underwent 90-min middle cerebral artery...
3D printing has emerged as an enabling approach in a variety of different fields. However, the bulk volume systems limits expansion their applications. In this study, portable Digital Light Processing (DLP) printer is built based on smartphone-powered projector and custom-written smartphone-operated app. Constructs with detailed surface architectures, porous features, or hollow structures, well sophisticated tissue analogs, are successfully printed using platform, by utilizing commercial...
Due to the poor mechanical properties of many hydrogel bioinks, conventional 3D extrusion bioprinting is usually conducted based on X-Y plane, where deposited layers are stacked in Z-direction with or without support prior layers. Herein, a technique reported, taking advantage cryoprotective bioink enable direct vertical direction presence cells, using freezing plate precise temperature control. Of interest, cryo-bioprinting concurrently allows user create freestanding filamentous constructs...
Biofabrication via light-based 3D printing offers superior resolution and ability to generate free-form architectures, compared conventional extrusion technologies. While extensive efforts in the design of new hydrogel bioinks lead major advances methods, accessibility lithographic bioprinting is still hampered by a limited choice cell-friendly resins. Herein, we report development novel set photoresponsive bioresins derived from ichthyic-origin gelatin, designed print high-resolution...
Abstract Digital light processing (DLP)‐based bioprinting technology has recently aroused considerable concerns as a strategy to deliver biomedical materials and/or specific cells create sophisticated structures for various tissue modeling and regeneration. In this review, we display concise introduction of DLP bioprinting, further discussion on the design manufacture (bio)printer with varied bioinks their applications toward drug screening, disease modeling, repair, regenerative medicine....
Abstract It is well‐known that tissue engineering scaffolds feature highly interconnected and size‐adjustable micropores are oftentimes desired to promote cellular viability, motility, functions. Unfortunately, the ability of precise control over microporous structures within bioinks in a cytocompatible manner for applications 3D bioprinting generally lacking, until method micropore‐forming bioink based on gelatin methacryloyl (GelMA) was reported recently. This took advantage unique aqueous...
Abstract Sacrificial printing allows introduction of architectural cues within engineered tissue constructs. This strategy adopts the use a 3D‐printed sacrificial ink that is embedded bulk hydrogel which subsequently dissolved to leave open‐channels. However, current conventional inks do not recapitulate dynamic nature development, such as temporal presentation matching cellular requirements during different stages maturation. To address this limitation, new class developed exhibits...
While there has been considerable success in the three-dimensional bioprinting of relatively large standalone filamentous tissues, fabrication solid fibers with ultrafine diameters or those cannular featuring ultrathin walls remains a particular challenge. Here, an enabling strategy for (bio)printing and hollow whose size ranges could be facilely adjusted across broad spectrum, is reported, using aqueous two-phase embedded approach combined specially designed cross-linking extrusion methods....
Abstract Cell‐laden hydrogel fibers/tubules are one of the fundamentals tissue engineering. They have been proven as a promising method for constructing biomimetic tissues, such muscle fibers, nerve conduits, tendon and vessels, etc. However, current fiber/tubule production methods limitations in ordered cell arrangements, thus impeding configurations. Acoustic patterning is manipulation that has good biocompatibility, wide tunability, contact‐free. there few studies on acoustic fiber...
Ischemic stroke causes a multitude of brain damage. Neurovascular injury and myelin sheath degradation are two manifestations ischemic Therapeutic strategies aiming only at repairing the neural components or vessels cannot efficiently restore neurological function. Endothelial progenitor cells (EPCs) have advantages both promoting angiogenesis secreting trophic factors that would promote neurogenesis. Chemokine cxcl12 gene therapy has also been shown to angiogenesis, neurogenesis,...
Dl-3-N-butylphthalide (NBP) is approved in China for the treatment of ischemic stroke. Previous studies have shown that NBP promotes recovery after stroke via multiple mechanisms. However, effect on vascular function and thrombosis remains unclear. Here, we aim to study using a rat model transient middle cerebral artery occlusion (MCAO) state-of-the-art high-resolution synchrotron radiation angiography. Eighty SD rats underwent MCAO surgery. (90 mg/kg) was administrated daily by gavage....
Neuroinflammation is the major pathogenesis of cerebral ischemia. Microglia are activated and polarized to either pro-inflammatory M1 phenotype or anti-inflammatory M2 phenotype, which act as a critical mediator neuroinflammation. Sestrin2 has pro-survival properties against ischemic brain injury. However, whether sestrin2 an function by shifting microglia polarization its underlying mechanism unknown.Adult male C57BL/6 mice (N = 108) underwent transient middle artery occlusion (tMCAO) were...
The COVID-19 pandemic has dramatically affected everyone's work and daily life, many employees are talking with their coworkers about this widespread on a regular basis. In research, we examine how crises such as at the team level affects dynamics behaviors. Drawing upon cultural tightness-looseness theory, propose that crisis among members is positively associated tightness, which in turn benefits teams by decreasing deviance but hurts creativity. Furthermore, suggest virtuality moderates...
Allicin has been reported to play a biological role in human pathophysiological processes via interaction with numerous signaling pathways and gene expression alteration. The purpose of the present study was evaluate protective effects allicin against renal ischemia/reperfusion injury (RIRI) rats. In study, RIRI model 45-min ischemia 22-h reperfusion rats generated used as intervention. Changes tissue pathomorphology, function, oxidative stress, inflammatory response apoptosis were evaluated...