A5101924166- T-cell and B-cell Immunology
- Redox biology and oxidative stress
- Immune Cell Function and Interaction
- Nanoplatforms for cancer theranostics
- Connective tissue disorders research
- Protease and Inhibitor Mechanisms
- Blood disorders and treatments
- Immunotherapy and Immune Responses
- Cancer, Hypoxia, and Metabolism
Kunming Medical University
2007-2019
Second Affiliated Hospital of Kunming Medical College
2007
Loyola University Chicago
2007
Abstract In this study, we aimed to explore the mechanism of glutathione peroxidase 3 (GPX3) in growth malignant melanoma (MM) cells by hypoxia‐inducible factor‐1α (HIF1‐α) and HIF2‐α regulating metabolism through reactive oxygen species (ROS). The messenger RNA protein expression GPX3, HIF1‐α, tissues, cell lines were measured reverse transcription‐quantitative PCR Western blot analysis. A375 transfected with GPX3 overexpression plasmid, small interfering (siRNA) targeting or siRNA...
Naive CD4(+) T cells were reported to produce small amounts of IL-4 in vitro, which are implicated be sufficient initiate T(h)2 response vivo. However, IL-4-producing naive difficult study vivo because they present low numbers shortly after the first antigen exposure. Here, we used IL-4/green fluorescence protein (GFP) reporter mice (G4 mice) track initial cells. We established a flow cytometry method estimate number GFP(+) demonstrated effectiveness this by showing that responding...