A5067160599- RNA Interference and Gene Delivery
- Crystallization and Solubility Studies
- Cancer-related molecular mechanisms research
- Cancer, Hypoxia, and Metabolism
- X-ray Diffraction in Crystallography
- Cervical Cancer and HPV Research
- Glioma Diagnosis and Treatment
- RNA modifications and cancer
- S100 Proteins and Annexins
- Advanced biosensing and bioanalysis techniques
- Virus-based gene therapy research
- Angiogenesis and VEGF in Cancer
- Plant-Microbe Interactions and Immunity
- Renal and related cancers
- Epigenetics and DNA Methylation
- Ferroptosis and cancer prognosis
- Circular RNAs in diseases
- Reproductive tract infections research
- Hepatitis B Virus Studies
- Mitochondrial Function and Pathology
- MicroRNA in disease regulation
- ATP Synthase and ATPases Research
- Renal cell carcinoma treatment
- Cancer Research and Treatments
- Extracellular vesicles in disease
Ningxia Center for Diseases Prevention and Control
2024
Kunming Medical University
2014-2023
Inner Mongolia Agricultural University
2023
Guangxi Normal University
2023
Second Affiliated Hospital of Kunming Medical College
2021
Fujian Medical University
2010-2021
Heilongjiang Bayi Agricultural University
2013-2021
Shanghai Jiao Tong University
2021
Shanghai First People's Hospital
2021
Soochow University
2021
Long noncoding RNAs (lncRNAs), a recently discovered class of cellular RNAs, play important roles in the regulation many developmental processes. Although lncRNAs have been systematically identified various systems, most them not functionally characterized vivo animal models. In this study, we 128 testis-specific Drosophila and knocked out 105 using an optimized three-component CRISPR/Cas9 system. Among lncRNA knockouts, 33 (31%) exhibited partial or complete loss male fertility, accompanied...
The exosome is a key initiator of pre-metastatic niche in numerous cancers, where macrophages serve as primary inducers tumor microenvironment. However, the proteome that can be exosomally transported from cancer cells to has not been sufficiently characterized so far. Here, we used colorectal (CRC) exosomes educate tumor-favorable macrophages. With SILAC-based mass spectrometry strategy, successfully traced CRC Such primarily focused on promoting cytoskeleton rearrangement, which was...
Soft rot caused by Pectobacterium carotovorum subsp. (Pcc) is a major constraint in the production of Chinese cabbage. The objective this study was to demonstrate that causative agent Pcc may be successfully managed Bacillus amyloliquefaciens KC-1, both vitro and vivo. cabbage seedlings were cultivated organic substrate termed bio-organic using floating-seedling system with B. KC-1. This approach applied greenhouse evaluate management soft rot. results showed extent rot, as well transmission...
Abstract In this study, we aimed to explore the mechanism of glutathione peroxidase 3 (GPX3) in growth malignant melanoma (MM) cells by hypoxia‐inducible factor‐1α (HIF1‐α) and HIF2‐α regulating metabolism through reactive oxygen species (ROS). The messenger RNA protein expression GPX3, HIF1‐α, tissues, cell lines were measured reverse transcription‐quantitative PCR Western blot analysis. A375 transfected with GPX3 overexpression plasmid, small interfering (siRNA) targeting or siRNA...
// Qiao Zhang 1, * , Zhe Yang 2, Qiaoqiao Han Honggang Bai 1 Yanling Wang Xiaojia Yi 3 Zihan Lijuan Lu Jiang Xin Song 4 Yingmin Kuang 5 and Yuechun Zhu Department of Biochemistry Molecular Biology, School Basic Medical Sciences, Kunming University, Chenggong District, 650500, Yunnan Province, China 2 Pathology, The First Affiliated Hospital Wuhua 650032, Second 650101, Cancer Biotherapy Center, Third Xishan 650118, Organ Transplantation, These authors have contributed equally to this work...
In this work, we report the direct diagnosing chemoresistance of glioma stem cells (GSCs) during chemotherapy on a biomimetric microsystem that reconstitutes perivascular niches chip. Glioma and endothelial were specially cocultured onto system to precisely control cell coculture for proof-of-principle studies. The expression levels 6-O-methylguanine was confirmed by mass spectrometer, Bmi-1 gene also investigated uncover GSCs. results demonstrated formation effectively maintains at...
Background Deterioration of the ecological environment in recent years has led to increasing soil salinization, which severely affects cultivation agricultural crops. While research focused on improving through application pollution-free microbial fertilizers, there are relatively few plant growth-promoting bacteria suitable for saline-alkali environments. Although Klebsiella variicola can adapt environments successfully colonize rhizosphere microenvironments, only a studies have...
HPV account for most of the incidence cervical cancer. Approximately 90% anal cancers and a smaller subset (<50%) other (oropharyngeal, penile, vaginal, vulvar) are also attributed to HPV. The L1 protein comprising vaccine formulations elicits high-titre neutralizing antibodies confers type restricted protection. L2 is promising candidate broadly protective vaccine. In our previous study, we found prevalent high-risk infectious serotypes were HPV-16 HPV-58 among women Southwest China. To...
Genetic variations of High‐Risk HPV E6/E7 may be associated with the development cervical cancer in specific geographic regions. Few data have been reported about prevalence and variants among patients Southwest China. This study was designed to investigate most prevalent After genotyping, genes HR samples were sequenced analyzed. Phylogenetic trees then constructed, followed by an analysis diversity secondary structure selection pressures. 16 (73.8%) 18 (16.4%) are infection types patients,...
In this study, a novel lipid vector has been developed for targeted delivery of oligodeoxynucleotides (ODN) to tumors that overexpress folate receptor. This is based on method by Semple et al. (1), which utilizes an ionizable aminolipid (1,2-dioleoyl-3-(dimethylammonio)propane, DODAP) and ethanol-containing buffer system encapsulating large quantities polyanionic ODN in vesicles. Folate incorporated into the vesicles via distearoylphosphatidylethanolamine−poly(ethylene glycol) (DSPE−PEG)...
Porcine reproductive and respiratory syndrome (PRRS) caused by PRRS virus (PRRSV) is an infectious disease characterized severe deficiency in pregnant sows, typical symptoms piglets, high mortality rate of piglets. In this study, we employed Affymetrix microarray chip to compare the gene expression profiles lung tissue samples from Dapulian (DPL) pigs (a Chinese indigenous pig breed) Duroc×Landrace×Yorkshire (DLY) after infection with PRRSV. During PRRSV, DLY exhibited a range clinical...
Abstract The regulatory relationship between silent information regulator 2 (SIRT2) and glucose 6‐phosphate dehydrogenase (G6PD) in clear cell renal carcinoma (ccRCC) is still unclear. present study aimed to explore the function of SIRT2 its effect on G6PD ccRCC. Cancer Genome Atlas data mining was first analyzed. Quantitative real‐time PCR western blot analyses were used assess mRNA protein expression levels, respectively. Cell viability, colony formation, cycle, apoptosis, TUNEL assays EdU...
CD133 has been identified as a cancer stem cell marker in colon and several other cancers, but its function is still unknown. We examined the expression 44 human lines, found five of 8 positive lines were from cancer. The subpopulation cells showed more vigorous growth lower differentiation. Induction differentiation reduced CD133-positive population. Knockdown could not induce cellular Care must be taken if used only cancer, especially established lines. negatively correlates with...
We reported previously that amoeboid microglial cells in the postnatal rat brain expressed 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) both vivo and vitro; however, functional role of CNPase microglia has remained uncertain. This study extended investigation to determine expression activated derived from cell culture animal models injury with objective clarify its putative functions.Three-day-old Wistar rats were given an intraperitoneal injection lipopolysaccharide induce...
A high-fat diet plays an important role in aggravating cancers. Palmitic acid (PA) is one of the components saturated fatty acids; it has been reported to promote tumor proliferation melanomas, but signal transduction pathway mediated by palmitic remains unclear. This study showed that can lung metastasis melanomas. Moreover, interaction between and toll-like receptor 4 (TLR4) was predicted molecular docking. The experimental results proved could TLR4 Toll/IL-1 domain-containing...