Acute myeloid leukemia (AML) is a heterogeneous group of hematopoietic malignancies due to sophisticated genetic mutations and epigenetic dysregulation. MicroRNAs (miRNAs), class small non-coding RNAs, are important regulators gene expression in all biological processes, including leukemogenesis. Recently, miR-375 has been reported be suppressive miRNA multiple types cancers, but its underlying anti-leukemia activity AML largely unknown. Quantitative reverse transcriptase PCR (qRT-PCR) was...

This article reports the clinical characteristics and treatment processes of three cases mucormycosis occurring after hematopoietic stem cell transplantation in children, along with a review relevant literature. All patients presented chest pain as initial symptom, metagenomic next-generation sequencing (mNGS) confirmed mucycete infection early all cases. Two recovered treatment, while one succumbed to disseminated infection. mNGS has facilitated diagnosis reducing mortality rates....

The metastatic cascade is a complex and multistep process with many potential barriers. Recently, miR-193a has been reported to be suppressive miRNA in multiple types of cancers, but its underlying anti-oncogenic activity non-small cell lung cancers (NSCLC) not fully elucidated. expressions (miR-193a-5p) human cancer tissues lines were detected by real-time PCR. Dual-luciferase reporter assay was used identify the direct target miR-193a. Cell proliferation, apoptosis, metastasis assessed...

Oxidative stress is considered a crucial mediator in the pathogenesis of various liver diseases. The flavone diosmetin has been reported to exhibit antioxidant activities; however, hepatoprotective effects against oxidative stress, and underlying molecular mechanisms, remain unknown. present study aimed investigate potential on hydrogen peroxide (H2O2)‑induced damage L02 cells attempted evaluate role nuclear factor erythroid 2‑related 2 (Nrf2)/antioxidant response element pathway this...

BACKGROUND:Breast cancer is one of the most malignant tumors worldwide. The natural flavonoid diosmetin has been reported to exhibit various pharmacological activities, including anti-cancer effects. This study aimed investigate anti-breast effects on MDA-MB-231 cells and explore underlying molecular mechanisms cell apoptosis. MATERIAL AND METHODS:The were incubated with for 24 h. Then, viability lactate dehydrogenase (LDH) leakage detected using CCK-8 LDH assay kits, respectively. Inverted...

CD133 has been identified as a cancer stem cell marker in colon and several other cancers, but its function is still unknown. We examined the expression 44 human lines, found five of 8 positive lines were from cancer. The subpopulation cells showed more vigorous growth lower differentiation. Induction differentiation reduced CD133-positive population. Knockdown could not induce cellular Care must be taken if used only cancer, especially established lines. negatively correlates with...

The stress distributions in a thick welded specimen with partial repair weld were measured the three-cut contour method. longitudinal maps original and obtained transverse map at centerline was acquired. difference between that investigated. Results show increases significantly within entire region peak tensile close to yield strength of material; above decreases distinctly after repair; introduction does not affect distribution trend (whether it is beyond or weld), has slight effect on width region.

Neural progenitor cells (NPCs) capable of self-renewal and differentiation into neural cell lineages offer broad prospects for therapy neurodegenerative diseases. However, based on NPC transplantation is limited by the inability to acquire sufficient quantities NPCs. Previous studies have found that a chemical cocktail valproic acid, CHIR99021, Repsox (VCR) promotes mouse fibroblasts differentiate NPCs under hypoxic conditions. Therefore, we used VCR (0.5 mM 3 μM 1 Repsox) induce...

Differences in individual drug responses are obstacles breast cancer (BRCA) treatment, so predicting would help to plan treatment strategies. The accumulation of molecular profiling and response data provide opportunities challenges identify novel signatures mechanisms tumor responsiveness drugs BRCA. This study evaluated with a multi-omics integrated system that depended on long non-coding RNAs (lncRNAs). We identified response-related lncRNAs (DRlncs) by combining expression lncRNA,...

Inflammation is essential for the pathological process of acute lung injury (ALI). Trilo-batin, a glycosylated dihydrochalcone can show anti-oxidative and anti-inflammation properties. This study aimed to explore whether trilobatin could suppress inflammation in lipopolysaccharide (LPS)-induced ALI. Firstly, mice were injected with intraperitoneally, then LPS was administered intranasally induce injury. Data from analysis edema pathologic histology tissues indicated that pretreatment...

Metabolic Syndrome (MS) is a rapidly growing medical problem worldwide and characterized by cluster of age-related metabolic risk factors. The presence MS increases the likelihood developing atherosclerosis significantly raises morbidity/mortality rate acute coronary syndrome (ACS) patients. Early detection crucial, biomarkers, particularly blood-based, play vital role in this process. This cross-sectional study focused on investigation certain plasma ceramides (Cer14:0, Cer16:0, Cer18:0,...

Allogeneic stem cell transplantation is a cure for patients suffering from thalassemia major (TM). Historically, were limited by the selection of donors, while advancement haploidentical (haplo-SCT) has greatly expanded donor pool. However, outcomes haplo-SCT in TM recipients vary between different programs. In this study, we retrospectively studied 73 pediatric (median age, 7 years; range, 3 to 14 years) who underwent haplo-cord transplantation. Both estimated overall survival and...

Previous reports have demonstrated that RepSox can function as a replacement for cMyc and Sox2 in the reprogramming of cells into induced pluripotent stem (iPSCs), well increasing levels bone morphogenetic protein (BMP)‑3 inducing phosphorylation Smad1 mouse embryonic cells. In present study, it was caused visible morphological transformation sheep fibroblasts; however, no significant alterations cell proliferation, apoptosis or chromosome aberrations were observed. Moreover, increased...

Astrocytoma is the most common glioma, accounting for half of all primary brain and spinal cord tumors. Late detection aggressive nature high-grade astrocytomas contribute to high mortality rates. Though many studies identify candidate biomarkers using high-throughput transcriptomic profiling stratify grades subtypes, few have resulted in clinically actionable results. This shortcoming can be attributed, part, pronounced lab effects that reduce signature robustness varied individual gene...

Abstract Previous reports have demonstrated that Reversine can reverse differentiation of lineage-committed cells to mesenchymal stem and suppress tumors growth. However, the molecular mechanisms antitumor activity promoting cellular dedifferentiation for reversine not yet been clearly elucidated. In present study, it was 5 μM could induce multinucleated through cytokinesis failure rather than just arrested in G2 or M phase. Moreover, reversed sheep fibroblasts into MSC-like style, notably...

It is the research focus of electronic reconnaissance at all levels to locate ground jamming source by using spacecraft equipped with direction finding system. Aiming characteristics working platform, localization algorithm single-satellite radiation based on studied. In simulation experiment, STK satellite orbit software used generate position data and azimuth angle target relative satellite. The are modeled unscented Kalman filter. positioning accuracy extended filter analyzed. And...

Drug-resistant cytomegalovirus (CMV) infection remains a challenge in the management of pediatric recipients hematopoietic stem cell transplantation (HSCT). In this study, we retrospectively reviewed clinical data on HSCT and identified known unknown drug-resistant CMV variants. A total 221 children underwent allogeneic between October 2017 November 2019 at Shenzhen Children's Hospital; these, 35 patients were suspected having infections tested for mutations UL97 UL54 genes by Sanger...

In order to study the heat transfer performance of solar horizontal pipes, a high-precision experimental apparatus for capacity pipe was developed. It proved that system balance is less than 10% and relative error ofthe 6%. By test, it shown when liquid level height working fluidwas about 19% ∼ 22% inner diameter optimal obtained.In addition, pipes affected adversely by installation angle.