A5057243932- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Plant biochemistry and biosynthesis
- Fungal Biology and Applications
- Neurogenesis and neuroplasticity mechanisms
- Microbial Natural Products and Biosynthesis
- Pluripotent Stem Cells Research
- Gut microbiota and health
- Circular RNAs in diseases
- Diet and metabolism studies
- Advanced biosensing and bioanalysis techniques
- Enzyme Production and Characterization
- Biofuel production and bioconversion
- Ubiquitin and proteasome pathways
- Cancer-related Molecular Pathways
- Microtubule and mitosis dynamics
- Olfactory and Sensory Function Studies
- RNA Interference and Gene Delivery
- Polysaccharides and Plant Cell Walls
- Microbial Metabolism and Applications
- Bacteriophages and microbial interactions
- Nerve injury and regeneration
- Cancer Genomics and Diagnostics
- Glycosylation and Glycoproteins Research
Northeast Forestry University
2015-2025
First Affiliated Hospital of Harbin Medical University
2013-2025
Anhui University of Traditional Chinese Medicine
2025
Zaozhuang University
2020-2025
Harbin University
2019-2025
Hainan Normal University
2025
Jiangnan University
2024
Capital University of Physical Education and Sports
2024
Bengbu Medical College
2021-2024
Fountain Biopharma (Taiwan)
2024
The discovery of RNAi has revolutionized loss-of-function genetic studies in mammalian systems. However, significant challenges still remain to fully exploit for genetics. For instance, screens and vivo could be broadly improved by methods that allow inducible uniform gene expression control. To achieve this, we built the lentiviral pINDUCER series vehicles vivo. Using a multicistronic design, enable tracking viral transduction shRNA or cDNA induction broad spectrum cell types They this...
Myc is an oncogenic transcription factor frequently dysregulated in human cancer. To identify pathways supporting the program, we used a genome-wide RNA interference screen to search for Myc-synthetic lethal genes and uncovered role SUMO-activating enzyme (SAE1/2). Loss of SAE1/2 enzymatic activity drives synthetic lethality with Myc. Inactivation SAE2 leads mitotic catastrophe cell death upon hyperactivation. Mechanistically, inhibition switches transcriptional subprogram from activated...
The aim of this study was to investigate the expression circulating microRNAs (miRNAs) in apolipoprotein E (apoE) knockout mice (apoE−/−) and validate role these miRNAs human coronary artery disease (CAD). Pooled plasma from 10 apoE−/− healthy C57BL/6 (B6) used perform microarray analysis. results showed that miR-34a, miR-21, miR-23a, miR-30a miR-106b were differentially expressed mice, changes confirmed by real-time quantitative reverse-transcription PCR. Then, detected 32 patients with CAD...
Alternative splicing (AS) allows generation of cell type–specific mRNA transcripts and contributes to hallmarks cancer. Genome‐wide analysis for AS in human hepatocellular carcinoma (HCC), however, is limited. We sought obtain a comprehensive landscape HCC define tumor‐associated variants. Single‐molecule real‐time long‐read RNA sequencing was performed on patient‐derived cells, presence splice junctions defined by SpliceMap‐LSC‐IDP algorithm. obtained an all‐inclusive map annotated variants...
Serratia marcescens has garnered increasing attention as a promising host for valuable compound production. However, the lack of an efficient gene regulation toolkit severely hampers its applications. Here, library stationary phase promoters was screened in S. HBA7 using RNA-seq and RT-qPCR, revealing 43-fold regulatory range with red fluorescent protein mKate2 reporter. The β-galactosidase employed to demonstrate universality driving expression different proteins. wide-ranging utility these...
Multiple lines of evidence suggest that the gut microbiota plays vital roles in metabolic diseases such as hyperlipidemia. Previous studies have confirmed krill oil can alleviate hyperlipidemia, but underlying mechanism remains unclear. To discern whether changes structure during hyperlipidemia treatment, 72 mice were acclimatized with a standard chow diet for 2 weeks and then randomly allocated to receive (control group, n = 12) or high-sugar-high-fat (HSHF) supplemented low (100 μg/g·d,...
Serratia marcescens is utilized as a significant enterobacteria in the production of various high-value secondary metabolites. Acetoin serves crucial foundational compound development and finds application broad range fields. Furthermore, S. HBQA-7 capable utilizing xylose its exclusive carbon source for acetoin production. The objective this study was to utilize constitutive promoter screening strategy enhance both utilization HBQA-7. By RNA-seq, we identified endogenous P6 that most...
Malignant glioma is the most common intracranial tumor with poor prognosis. It well believed that stem cells (GSCs) are responsible for initiation and progression of glioma. Janus kinase/signal transducer activator transcription (Jak/STAT3) pathway plays a key role in functions GSCs. However, regulatory mechanism Jak/STAT3 has not been completely elucidated. This study employed multidisciplinary approaches to investigate upstream regulators signaling miR-30 was found be overexpressed GSCs...
Abstract Stem cell transplantation has been shown to improve functional outcome in degenerative and ischemic disorders. However, low vivo survival differentiation potential of the transplanted cells limits their overall effectiveness thus clinical usage. Here we show that, after vitro induction neuronal dedifferentiation, on withdrawal extrinsic factors, mesenchymal stem (MSCs) derived from bone marrow, which have already committed lineage, revert a primitive population (dedifferentiated...
Abstract Background Gastric cancer (GC) is confronted with limited options for precision medicine. Human epidermal growth factor receptor 2 (HER2) the principal druggable target of GC, yet proper biomarkers response/resistance prediction remain unveiled. Methods From 40 GC patients received HER2‐targeted therapy, a total 327 peripheral blood plasma specimens was collected including baseline and treatment time points. Circulating tumor DNA (ctDNA) extracted sequenced panel 425 genes....
Background: Basic helix–loop–helix (bHLH) transcription factors (TFs) widely exist in eukaryotic organisms and play a key role plant growth development response to environmental stresses. Sanghuangporus baumii, an important medicinal mushroom known for its anticancer properties, has limited research on the bHLH gene family. Methods: This utilized genomic data from S. baumii identify family members, their structure, conserved motifs, phylogenetic relationship were characterized. Additionally,...
Electroacupuncture (EA) demonstrates neuroprotective in cerebral ischemia–reperfusion (I/R) injury. N6-methyladenosine (m6A) is found to contribute the pathogenesis of neurological conditions recently. The objective this study investigate effects EA on m6A and related mechanism I/R After middle artery occlusion/reperfusion (MCAO/R) operation was used establish rat models with injury, applied Baihui (GV20) Dazhui (GV14) once daily for 7 consecutive days. Subsequently, modified Neurological...
To address environmental challenges such as eutrophication caused by excessive phosphorus discharge and low resource utilization of solid waste, this study investigates the factors influencing physical properties ceramsite from waste (SW-ceramsite) through sintering method, its adsorption performance. The results shown that under optimal conditions additive dosage 9 g/100 g mixture, temperature 1100 °C, heating rate 10 °C/min, holding time 5 min, values breaking wear rates void fraction were...