A5066098323- Estrogen and related hormone effects
- Epigenetics and DNA Methylation
- Advanced Breast Cancer Therapies
- Ferrocene Chemistry and Applications
- Histone Deacetylase Inhibitors Research
- HER2/EGFR in Cancer Research
- FOXO transcription factor regulation
- Genomics and Chromatin Dynamics
- Composting and Vermicomposting Techniques
- Monoclonal and Polyclonal Antibodies Research
- Lung Cancer Research Studies
- Hormonal Regulation and Hypertension
- Cancer-related Molecular Pathways
- Optical Coatings and Gratings
- Ubiquitin and proteasome pathways
- Cancer Cells and Metastasis
- Constructed Wetlands for Wastewater Treatment
- PI3K/AKT/mTOR signaling in cancer
- Cytokine Signaling Pathways and Interactions
- Pharmaceutical and Antibiotic Environmental Impacts
- Water Quality Monitoring and Analysis
- Prostate Cancer Treatment and Research
- Remote Sensing and Land Use
- Photonic and Optical Devices
- Aquatic Ecosystems and Phytoplankton Dynamics
Peking University People's Hospital
2025
Peking University
2025
Hebei University of Technology
2025
Gilead Sciences (United States)
2017-2025
Southwest Forestry University
2009-2024
Baylor College of Medicine
2014-2023
Lanzhou Jiaotong University
2012-2022
Dan L Duncan Comprehensive Cancer Center
2022
Baylor University
2021
Zhejiang University of Technology
2021
The discovery of RNAi has revolutionized loss-of-function genetic studies in mammalian systems. However, significant challenges still remain to fully exploit for genetics. For instance, screens and vivo could be broadly improved by methods that allow inducible uniform gene expression control. To achieve this, we built the lentiviral pINDUCER series vehicles vivo. Using a multicistronic design, enable tracking viral transduction shRNA or cDNA induction broad spectrum cell types They this...
Abstract Introduction The human epidermal growth factor receptor 2 (HER2)-targeted therapies trastuzumab (T) and lapatinib (L) show high efficacy in patients with HER2-positive breast cancer, but resistance is prevalent. Here we investigate mechanisms to each drug alone, or their combination using a large panel of cell lines made resistant these drugs. Methods Response L + T treatment was characterized 13 identify that were de novo resistant. Acquired then established by long-term exposure...
Accumulating evidence suggests that both levels and activity of the estrogen receptor (ER) progesterone (PR) are dramatically influenced by growth-factor (GFR) signaling pathways, this crosstalk is a major determinant breast cancer progression response to therapy. The phosphatidylinositol 3-kinase (PI3K) pathway, key mediator GFR signaling, one most altered pathways in cancer. We thus examined whether deregulated PI3K luminal ER+ tumors associated with ER level intrinsic molecular subtype....
Tamoxifen has been a frontline treatment for estrogen receptor alpha (ERα)-positive breast tumors in premenopausal women. However, resistance to tamoxifen occurs many patients. ER still plays critical role the growth of cancer cells with acquired resistance, suggesting that ERα remains valid target tamoxifen-resistant (Tam-R) cancer. In an effort identify novel regulators signaling, through small-scale siRNA screen against histone methyl modifiers, we found WHSC1, H3K36 methyltransferase, as...
Forkhead box protein A1 (FOXA1) is a pioneer factor of estrogen receptor α (ER)-chromatin binding and function, yet its aberration in endocrine-resistant (Endo-R) breast cancer unknown. Here, we report preclinical evidence for role FOXA1 Endo-R as well clinical significance. gene-amplified and/or overexpressed derivatives several cell line models. Induced triggers oncogenic gene signatures proteomic profiles highly associated with endocrine resistance. Integrated omics data reveal IL8 one...
Traditionally, GRP78 has been regarded as an endoplasmic reticulum (ER) lumenal protein due to its carboxyl KDEL retention motif. Recently, a subfraction of is found localize the surface specific cell types, serving co-receptors and regulating signaling. However, physiological relevance (sGRP78) expression in cancer functional interactions at are just emerging. In this report, we combined biochemical, imaging mutational approaches address these issues. For detection sGRP78, utilized mouse...
Forkhead box A1 (FOXA1) is a pioneer factor that facilitates chromatin binding and function of lineage-specific oncogenic transcription factors. Hyperactive FOXA1 signaling due to gene amplification or overexpression has been reported in estrogen receptor-positive (ER+) endocrine-resistant metastatic breast cancer. However, the molecular mechanisms by which up-regulation promotes these processes key downstream targets network remain elusive. Here, we demonstrate ER+ cancer cells drives...
To investigate the direct effect and therapeutic consequences of epidermal growth factor receptor 2 (HER2)-targeting therapy on expression estrogen (ER) Bcl2 in preclinical models clinical tumor samples.
The estrogen receptor (ER) drives the growth of most luminal breast cancers and is primary target endocrine therapy. Although ER blockade with drugs such as tamoxifen very effective, a major clinical limitation development resistance especially in setting metastatic disease. Preclinical observations suggest that even following resistance, signaling continues to exert pivotal role tumor progression majority cases. Through analysis cistrome tamoxifen-resistant cancer cells, we have uncovered...
Resistance to cancer treatment can be driven by epigenetic reprogramming of specific transcriptomes in favor the refractory phenotypes. Here we discover that tamoxifen resistance breast is a regulatory axis consisting master transcription factor, its cofactor, and an regulator. The oncogenic histone methyltransferase EZH2 conferred silencing expression estrogen receptor α (ERα) cofactor GREB1. In clinical specimens, induction DNA methylation particular CpG-enriched region at GREB1 promoter...
Cyclin-dependent kinase 4 (CDK4) and CDK6 inhibitors (CDK4/6i) are highly effective against estrogen receptor-positive (ER+)/HER2- breast cancer; however, intrinsic acquired resistance is common. Elucidating the molecular features of sensitivity to CDK4/6i may lead identification predictive biomarkers novel therapeutic targets, paving way toward improving patient outcomes.Parental cancer cells their endocrine-resistant derivatives (EndoR) were used. Derivatives with palbociclib (PalboR)...
Breast cancer (BCa) molecular subtypes include luminal A, B, normal-like, HER-2–enriched, and basal-like tumors, among which B cancers are highly aggressive. Biochemical pathways associated with patient survival or treatment response in these more aggressive not well understood. With the limited availability of pathologically verified clinical specimens, cell line models routinely used for pathway-centric studies. We measured metabolome BCa lines using mass spectrometry, linked metabolites...
Abstract Recent studies have demonstrated that chromatin architecture is linked to the progression of cancers. However, roles 3D structure and its dynamics in hormone-dependent breast cancer endocrine resistance are largely unknown. Here we report across a time course estradiol (E2) stimulation human estrogen receptor α (ERα)-positive cells. We identified subsets temporally highly dynamic compartments predominantly associated with active open found these showed higher alteration...
Background & Aims: RNA interference (RNAi) is a powerful tool to silence gene expression. The adenoviral vector expressing small interfering (siRNA) highly effective in mammalian cells. However, its potential use as therapeutic target an oncogene specifically remains be seen. We applied the adenovirus-delivered siRNA (AdSiRNA) inhibit hepatocellular carcinoma (HCC) oncogene, p28GANK, HCC cell lines and investigated antitumor effects. Methods: T7-RNA polymerase system was used screen specific...
In unstressed cells, the tumor suppressor p53 is maintained at low levels by ubiquitin-mediated proteolysis mainly through Mdm2. response to DNA damage, stabilized and becomes activated turn on transcriptional programs that are essential for cell cycle arrest apoptosis. Activation of leads accumulation Mdm2 protein, a direct target p53. It not understood how protected from degradation when up-regulated. Here we report stabilization in late phase after ionizing radiation correlates with...