A5034015616- Science, Research, and Medicine
- Cancer Genomics and Diagnostics
- Nutrition, Genetics, and Disease
- Advanced biosensing and bioanalysis techniques
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Genomic variations and chromosomal abnormalities
- DNA Repair Mechanisms
- Ferroptosis and cancer prognosis
- Electrochemical Analysis and Applications
- Genomics and Phylogenetic Studies
- Biosensors and Analytical Detection
- Cancer Cells and Metastasis
- Chromosomal and Genetic Variations
- Tribology and Wear Analysis
- Cancer-related molecular mechanisms research
- Influenza Virus Research Studies
- Metal-Organic Frameworks: Synthesis and Applications
- Hepatocellular Carcinoma Treatment and Prognosis
- Gold and Silver Nanoparticles Synthesis and Applications
- Cancer-related gene regulation
- Genomics and Rare Diseases
- Genetic factors in colorectal cancer
- RNA and protein synthesis mechanisms
- Petroleum Processing and Analysis
University of Macau
2023-2025
Suzhou Institute of Systems Medicine
2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2025
Beijing Biocytogen (China)
2024-2025
University of Jinan
2023-2024
Chinese University of Hong Kong
2019-2024
Henan University of Science and Technology
2024
Prince of Wales Hospital
2022-2023
Beijing University of Chemical Technology
2007-2023
Capital Normal University
2011-2019
Exosomes are increasingly recognized as important mediators of cell-cell communication in cancer progression through the horizontal transfer RNAs and proteins to neighboring or distant cells. Hepatocellular carcinoma (HCC) is a highly malignant cancer, whose metastasis largely influenced by tumor microenvironment. The possible role exosomes interactions between HCC cell its surrounding hepatic milieu however unknown. In this study, we comprehensively characterized exosomal RNA proteome...
Background & AimsIntratumor heterogeneity and divergent clonal lineages within among primary recurrent hepatocellular carcinomas (HCCs) produce challenges to patient management. We investigated genetic epigenetic variations liver tumors, hepatic lesions, between relapsing tumors.MethodsTumor matched nontumor specimens were collected from 113 patients who underwent partial hepatectomy for or HCC at 2 hospitals in Hong Kong. performed whole-genome, whole-exome, targeted capture sequencing...
Abstract Notch signaling represents a key mechanism mediating cancer metastasis and stemness. To understand how is overactivated to couple tumor self-renewal in NSCLC cells, we performed the current study showed that RFC4, DNA replication factor amplified more than 40% of tissues, directly binds Notch1 intracellular domain (NICD1) competitively abrogate CDK8/FBXW7-mediated degradation NICD1. Moreover, RFC4 functional transcriptional target gene signaling, forming positive feedback loop...
The importance of mRNA N6-methyladenosine (m6 A) modification during tumor metastasis is controversial as it plays distinct roles in different biological contexts. Moreover, how cancer cell plasticity shaped by m6 A interesting but remains uncharacterized. Here, this work shows that reader insulin like growth factor 2 binding protein 3 (IGF2BP3) remarkably upregulated metastatic lung adenocarcinoma (LUAD) and indicates worse prognosis patients. Interestingly, IGF2BP3 induces partial...
The ubiquitin receptor ADRM1/Rpn13 governs the specificity of eukaryotic protein degradation. By SMRT sequencing, we first discovered a novel spliced variant ADRM1 with skipped exon 9, termed ADRM1-ΔEx9, in human hepatocellular carcinoma (HCC). This study aimed to elucidate this receptor's underlying biology and clinical implications HCC. role ADRM1-ΔEx9 early liver carcinogenesis was studied using liver-derived non-tumoral organoids murine model hydrodynamic vivo transfection. HCC its...
Astrocyte elevated gene 1 (AEG-1), a novel oncoprotein, has been implicated in oncogenesis and cancer progression various types of human cancers. The clinical significance biological role AEG-1 non-small cell lung (NSCLC), however, remain unclear. In the present study, we found that expression was markedly up-regulated NSCLC lines tissues at level both transcription translation. Ectopically expressed enhanced migratory invasive abilities cells, whereas knockdown endogenous by specific shRNAs...
Alternative splicing (AS) allows generation of cell type–specific mRNA transcripts and contributes to hallmarks cancer. Genome‐wide analysis for AS in human hepatocellular carcinoma (HCC), however, is limited. We sought obtain a comprehensive landscape HCC define tumor‐associated variants. Single‐molecule real‐time long‐read RNA sequencing was performed on patient‐derived cells, presence splice junctions defined by SpliceMap‐LSC‐IDP algorithm. obtained an all‐inclusive map annotated variants...
Background and Aims: Major genomic drivers of hepatocellular carcinoma (HCC) are nowadays well recognized, although models to establish their roles in human HCC initiation remain scarce. Here, we used liver organoids experimental systems mimic the early stages carcinogenesis from genetic lesions TP53 loss L3 loop R249S mutation. In addition, chromatin immunoprecipitation sequencing (ChIP‐seq) cell lines shed important functional insights into consequential tumor‐suppressive function...
The hepatic manifestation of the metabolic syndrome, non-alcoholic fatty liver disease (NAFLD), can lead to development hepatocellular carcinoma (HCC). Despite a strong causative link, NAFLD-HCC is often underrepresented in systematic genome explorations.Herein, tumor-normal pairs from 100 patients diagnosed with were subject next-generation sequencing. Bioinformatic analyses performed identify key genomic, epigenomic and transcriptomic events associated pathogenesis NAFLD-HCC. Establishment...
Abstract Background Cancer‐associated fibroblasts (CAFs), integral to the tumour microenvironment, are pivotal in cancer progression, exhibiting either pro‐tumourigenic or anti‐tumourigenic functions. Their inherent phenotypic and functional diversity allows for subdivision of CAFs into various subpopulations. While several classification systems have been suggested different types, a unified molecular on single‐cell pan‐cancer scale has yet be established. Methods We employed comprehensive...
A facile microarray-based fluorescent sensor for the detection of lead (II) was developed based on catalytic cleavages substrates by a DNAzyme upon its binding to Pb(2+). The release fluorophore labelled resulted in decrease fluorescence intensity. had quantifiable range from 1 nM μM and selectivity >20 fold Pb(2+) over other metal ions.
It is quite challenging to improve the binding affinity of antismall molecule aptamers. We report that anticocaine split aptamer pairs improved by up 66-fold gold nanoparticles (AuNP)-attached aptamers due substantially increased local concentration and multiple simultaneous ligand interactions. The significantly enables detection small targets with unprecedented sensitivity, as demonstrated in nanoprobe-enhanced aptamer-based electrochemical sandwich assays (NE-SAESA). NE-SAESA replaces...
Epidemiological studies have demonstrated the embryonic and developmental toxicity of plasticizers. Thus, understanding in utero biotransformation accumulation plasticizers is essential to assessing their fate potential early life. In present study, 311 infant hair samples 271 paired meconium were collected at birth Guangzhou, China, characterize fetal exposure legacy emerging metabolites. Results showed that most target detected hair, with medians 9.30, 27.6, 0.145 ng/g for phthalate esters...
Abstract Background Genome stability is maintained by the DNA damage repair (DDR) system composed of multiple pathways hundreds genes. Germline pathogenic variation (PV) in DDR genes damages function affected genes, leading to genome instability and high risk diseases, particular, cancer. Knowing evolutionary origin PVs human essential understand etiology diseases. However, answer issue remains largely elusive. In this study, we analyzed for Methods We identified 169 referring various...
Purpose Hepatocellular carcinoma (HCC) is the sixth most common solid tumor worldwide and third leading cause of cancer-related death. HCC a particularly serious threat to Chinese population. Although many molecular alterations are known be involved in tumorigenesis hepatocytes, no systemic survey has examined somatic mutations samples from patients. Our goal was elucidate patients investigate possible mechanisms tumorigenesis. Experimental Design A total 110 hepatitis B virus (HBV)-positive...
The controlled attachment of thiolated DNA to gold nanoparticles (AuNPs) dictates many applications. This is typically achieved by either "aging-salting" processes or low-pH method, where Na+ H+ used minimize charge repulsion and facilitate onto AuNPs. However, the process takes a long time, prone aggregation when with larger Surfactants are needed precoat thereby enhance stability method can disrupt structural integrity DNAs. We report here an oligoethylene glycol (OEG) spacer-assisted that...
Castration-resistant prostate cancer (CRPC) is the leading cause of (PCa)-related death in males, which occurs after failure androgen deprivation therapy (ADT).PIWI-interacting RNAs (piRNAs) are crucial regulators many human cancers, but their expression patterns and roles CRPC remain unknown.In this study, we performed small RNA sequencing to explore CRPC-associated piRNAs using 10 benign tissues, 9 paired hormone-sensitive PCa (HSPCa) tissues from same patients.PiRNA-4447944 (piR-4447944)...
The kinetic adsorption profile at the DNA–gold nanoparticle (AuNP) interface is probed by following binding and organization of thiolated linear DNA aptamers varying chain lengths (15, 30, 44, 51 mer) to surface AuNPs (13.0 ± 1.0 nm diameter). A systematic investigation utilizing dynamic light scattering has been performed directly measure changes in particle size during course a typical aging-salting DNA/AuNP preparation procedure. We discuss effect length, composition, salt concentration,...
We report here a modified aptamer selection method, magnetic cross-linking precipitation (MCP)-SELEX, for highly efficient library enrichment and isolation. MCP-SELEX isolates bound aptamers via chemical between amino groups of target proteins activated carboxylic acid on beads (>90% coupling efficiency). Importantly, avoids surface interferences in conventional target-fixed methods substantially minimizes nonspecific binding. The efficiencies various (PD-L1, ubiquitin, thrombin, HSA) were...