A5065223164- Ion channel regulation and function
- Prostate Cancer Treatment and Research
- Neuroscience and Neuropharmacology Research
- Neuroscience and Neural Engineering
- Cancer-related molecular mechanisms research
- Nicotinic Acetylcholine Receptors Study
- Immunotherapy and Immune Responses
- Pancreatic function and diabetes
- Fibroblast Growth Factor Research
- Cancer Immunotherapy and Biomarkers
- MicroRNA in disease regulation
- Neurobiology and Insect Physiology Research
- Ubiquitin and proteasome pathways
- Chemokine receptors and signaling
- Cancer-related gene regulation
- Cytokine Signaling Pathways and Interactions
- Hormonal and reproductive studies
- Photoreceptor and optogenetics research
- Inflammatory Biomarkers in Disease Prognosis
- Cardiac electrophysiology and arrhythmias
- interferon and immune responses
- Wnt/β-catenin signaling in development and cancer
- Kruppel-like factors research
- Lipid Membrane Structure and Behavior
- Atherosclerosis and Cardiovascular Diseases
Innsbruck Medical University
2008-2021
Universität Innsbruck
2015-2021
University College London
2013
Abstract The normal prostate as well early stages and advanced cancer (PCa) require a functional androgen receptor (AR) for growth survival. recent discovery of microRNAs (miRNAs) novel effector molecules AR disclosed the existence an intricate network between AR, miRNAs downstream target genes. In this study DUCaP cells, characterized by high content wild-type robust transcriptional activity, were chosen main experimental model. By integrative analysis chromatin...
Significance Voltage-gated calcium channels are important regulators of neuronal functions, as for example synaptic transmission. Their auxiliary α 2 δ subunits modulating the currents. Beyond that they have emerged modulators functions. Here, we established a cellular triple knockout/knockdown model in cultured hippocampal neurons by knocking out or down expression all three expressed brain. Our experiments demonstrate presynaptic loss proteins leads to severe defect glutamatergic synapse...
Auxiliary beta subunits modulate current properties and mediate the functional membrane expression of voltage-gated Ca(2+) channels in heterologous cells. In brain, all four isoforms are widely expressed, yet little is known about their specific roles neuronal functions. Here, we investigated targeting role Ca(V)1.2 alpha(1) cultured hippocampal neurons. Quantitative reverse transcription-PCR showed equal expression, immunofluorescence a similar distribution endogenous throughout dendrites...
Novel drugs like Abiraterone or Enzalutamide, which target androgen receptor (AR) signaling to improve deprivation therapy (ADT), have been developed during the past years. However, application of these is limited because occurrence inherent acquired resistances treatment. Thus, identification new molecular targets urgently required current therapeutic prostate cancer (PCa) treatment strategies. PIAS1 (protein inhibitor activated STAT1 (signal transducer and activator transcription-1)) known...
The α2δ-1 protein is an auxiliary subunit of voltage-gated calcium channels, critical for neurotransmitter release. It upregulated in dorsal root ganglion (DRG) neurons following sensory nerve injury, and also the therapeutic target gabapentinoid drugs, which are efficacious both experimental human neuropathic pain conditions. has 3 spliced regions: A, B, C. A C cassette exons, whereas B introduced via alternative 3' splice acceptor site. Here we have examined presence variants DRG neurons,...
Genome-wide association studies have identified genomic loci, whose single-nucleotide polymorphisms (SNPs) predispose to prostate cancer (PCa). However, the mechanisms of most these variants are largely unknown. We integrated chromatin-immunoprecipitation-coupled sequencing and microarray expression profiling in TMPRSS2-ERG gene rearrangement positive DUCaP cells with GWAS PCa risk SNPs catalog identify disease susceptibility localized within functional androgen receptor-binding sites...
Development and progression of prostate cancer (PCa) are associated with chronic inflammation. The cytokine interleukin 6 (IL6) can influence progression, differentiation, survival, angiogenesis PCa. To identify novel pathways that triggered by IL6, we performed a gene expression profiling two PCa cell lines, LNCaP MDA 2b, treated 5 ng/ml IL6. Interferon (IFN) regulatory factor 9 (IRF9) was identified as one the most prevalent IL6-regulated genes in both lines. IRF9 is mediator type I IFN...
Abstract Sprouty proteins act as negative feedback inhibitors of fibroblast growth factor (FGF) signaling. FGFs belong to the neurotrophic factors and are involved in axonal during development repair. We investigated expression isoforms hippocampal neurons well regulation Sprouty2 ‐4 their role axon growth. were located nucleus, cytoplasm, dendrites, axons concentrated cones. During vivo differentiation vitro, was gradually downregulated neurons. Between 5 24 days culture both reduced by...
Background Chronic inflammation is frequently observed on histological analysis of malignant and non-malignant prostate specimens. It a suspected supporting factor for diseases their progression main cause false positive PSA tests in cancer screening. We hypothesized that induces autoantibodies, which may be useful biomarkers. aimed to identify validate associated serum autoantibodies patients evaluate the expression corresponding autoantigens. Methods Radical prostatectomy specimens (N =...
In nerve cells the genes encoding for α 2 δ subunits of voltage-gated calcium channels (VGCCs) have been linked to synaptic functions and neurological disease. Here we show that are essential formation organization glutamatergic synapses. Using a cellular subunit triple loss-of-function model, demonstrate failure in presynaptic differentiation associated with downscaling postsynaptic AMPA receptors density. The role isoforms as organizers is highly redundant, each individual isoform can...
Abstract In the prostate, normal glands, glandular hyperplasia, and adenocarcinoma are often accompanied by inflammation. Infiltrating inflammatory cells may affect either promote their proliferation or, alternatively act in tumor suppression. To better understand function of development, type number proximity to structures have be analyzed situ correlated with disease state. replace time-consuming, error-prone human evaluation stained tissue sections, we aimed at developing an automated...
Abstract Background: Chronic prostatic inflammation of the prostate is one main reasons for false positive serum PSA tests in cancer screening. In addition there accumulating evidence that an inflammatory microenvironment may support development malignancy and progression to metastatic disease. Easy accessible non-invasive indicators would be very helpful a biopsy decision after elevated test result. However, at present, histological examination biopsies remains only way identification...