A5043086600- Prostate Cancer Treatment and Research
- Cytokine Signaling Pathways and Interactions
- Renal Transplantation Outcomes and Treatments
- Cancer, Lipids, and Metabolism
- Estrogen and related hormone effects
- Organ Transplantation Techniques and Outcomes
- Multiple Myeloma Research and Treatments
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Cancer, Hypoxia, and Metabolism
- Cell death mechanisms and regulation
- Prostate Cancer Diagnosis and Treatment
- Organ Donation and Transplantation
- Drug Transport and Resistance Mechanisms
- Cholesterol and Lipid Metabolism
- Radiopharmaceutical Chemistry and Applications
- Liver Disease and Transplantation
- Biomarkers in Disease Mechanisms
- Medicinal Plant Pharmacodynamics Research
- Cancer Cells and Metastasis
- Pediatric Urology and Nephrology Studies
- Urological Disorders and Treatments
- Renal Diseases and Glomerulopathies
- Diabetes Treatment and Management
- Ubiquitin and proteasome pathways
- Hormonal and reproductive studies
Innsbruck Medical University
2016-2025
Universität Innsbruck
2014-2025
Organización Nacional de Trasplantes
2023
Hypertension Institute
2015
Docetaxel is a standard chemotherapy for patients with metastatic prostate cancer. However, the response rather limited and not all of benefit from this treatment. To uncover key mechanisms docetaxel insensitivity in cancer, we have established docetaxel-resistant sublines. In study, report that cells underwent an epithelial-to-mesenchymal transition during selection process, leading to diminished E-cadherin levels up-regulation mesenchymal markers. Screening regulators epithelial phenotype...
It is hypothesized that ligand-independent activation of the androgen receptor one mechanisms implicated in tumour progression. However, supportive evidence limited to effect HER-2/neu stimulates prostate cancer progression through receptor. In present study, we have asked whether proinflammatory cytokine interleukin-6 (IL-6), which known stimulate activity and expression its downstream target genes, may also induce growth androgen-sensitive cells. We found IL-6 differentially regulates...
Abstract Background Androgen receptor targeted therapies have emerged as an effective tool to manage advanced prostate cancer (PCa). Nevertheless, frequent occurrence of therapy resistance represents a major challenge in the clinical management patients, also because molecular mechanisms behind are not yet fully understood. In present study, we therefore aimed identify novel targets intervene with using gene expression analysis PCa co-culture spheroids where cells grown presence...
Abstract Background Immunosuppression after kidney transplantation is mainly guided via plasma tacrolimus trough level, which cannot sufficiently predict allograft rejection and infection. The load of the non-pathogenic highly prevalent torque teno virus (TTV) associated with immunosuppression its host. Non-interventional studies suggest use TTV to primary objective current trial demonstrate safety, tolerability preliminary efficacy TTV-guided immunosuppression. Methods For this purpose, a...
Abstract The potent immunomodulator FTY720 elicits immunosuppression via acting on sphingosine 1‐phosphate receptors (S1PR), thereby leading to an entrapment of lymphocytes in the secondary lymphoid tissue. To elucidate potential vitro effects this drug human monocyte‐derived DC, we used low nanomolar therapeutic concentrations and phosphorylated (FTY720‐P) investigated their influence DC surface marker expression, protein levels S1PR effector functions: antigen uptake, chemotaxis, cytokine...
Monoclonal gammopathy of undetermined significance (MGUS) is a premalignancy preceding multiple myeloma (MM) or related disorders. In MGUS, renal impairment caused by deposition the monoclonal immunoglobulins free light-chains (MGRS) often associated with high morbidity and mortality. We analysed prevalence impairment, clinical features long-term outcome in 2935 patients MGUS.Between 1/2000 8/2016, adult MGUS were identified our database.In 44/2935 (1.5%) MGRS was diagnosed. patients,...
Androgen receptor (AR) targeting remains the gold standard treatment for advanced prostate cancer (PCa); however, resistance a major clinical problem. To study therapeutic effects of clinically used anti-androgens we characterized herein tissue-mimetic three-dimensional (3D) in vitro model whereby PCa cells were cultured alone or with PCa-associated fibroblasts (CAFs). Notably, ratio to CAFs significantly increased time favor tumor within spheroids strongly mimicking vivo. Despite this loss...
In an interesting study, authors from Austria attempted to elucidate how often detrusor overactivity persists after TURP, and if perfusion of the lower urinary tract influences outcome. They found that increased vascular resistance bladder vessels leads reduced perfusion, provide a possible explanation for persistent symptoms. multicentre, community‐based randomized study conducted in USA UK, transdermal oxybutynin system improved quality life adults with overactive bladder. The final paper...
Suppressor of cytokine signaling-3 (SOCS-3) acts as a negative feedback regulator the Janus-activated kinase/signal transducers and activators transcription factors signaling pathway plays an important role in development progression various cancers. To better understand SOCS-3 prostate cancer, expression was down-regulated DU-145, LNCaP-IL-6+, PC3 cells by consecutive small interfering RNA transfections. mRNA protein measured quantitative reverse transcription-PCR Western blot,...
Two randomized trials demonstrated a survival benefit of triplet therapy (androgen deprivation [ADT]) plus androgen receptor pathway inhibitor [ARPI] docetaxel) over doublet (ADT docetaxel), thus changing treatment strategies in metastatic hormonesensitive prostate cancer (mHSPC).
Suppressor of cytokine signaling (SOCS) proteins play a pivotal role in the development and progression various cancers. We have previously shown that SOCS-3 is expressed prostate cancer, its expression inversely correlated with activation signal transducer activator transcription factor 3. hypothesized SOCS-1, if cancer cells, has growth-regulatory this malignancy. The presence both SOCS-1 mRNA protein was detected all tested cell lines. To assess levels vivo, we analyzed tissue microarrays...
Novel drugs like Abiraterone or Enzalutamide, which target androgen receptor (AR) signaling to improve deprivation therapy (ADT), have been developed during the past years. However, application of these is limited because occurrence inherent acquired resistances treatment. Thus, identification new molecular targets urgently required current therapeutic prostate cancer (PCa) treatment strategies. PIAS1 (protein inhibitor activated STAT1 (signal transducer and activator transcription-1)) known...
SEW2871 is a potent sphingosine-1-phosphate receptor type-1 (S1P1)-selective agonist that induces peripheral lymphopenia through sequestration of lymphocytes into secondary lymphoid organs, similar to the non-selective (S1P) FTY720. FTY720 has been reported interfere with human dendritic cell (DC) effector functions and both have shown modulate murine DC trafficking in vivo. Little known about possible effects on functions. Here, we demonstrate contrast FTY720, does not induce...
Abstract BACKGROUND Steroid receptor coactivators p300 and CBP are highly expressed in advanced prostate cancer. They potentiate activation of androgen by androgens anti‐androgens. In the present study, we have addressed question whether these enhance activity estrogen receptor‐beta (ER‐β), which is variably cancers. METHODS Expression levels were manipulated plasmid or siRNA transfections ER‐β was measured luciferase assays. Viability MTT assays cellular migration determined wound‐healing...
Kidneys from brain-death small pediatric donors ≤2 years are still classified as marginal organs. Herein, we analyse the outcomes following en-bloc kidney transplantation (EBKT) into adult recipients compared to standard criteria donor transplant (SKTs). A retrospective single center analysis of a prospectively collected and auditable database identified six EBKTs 75 SKTs between January 2015 June 2017. Propensity score matching minimized selection bias. After median follow-up 74 months,...
Suppressors of cytokine signalling (SOCS) are induced by interleukins (ILs) and various peptide hormones may prevent sustained activation pathways. We have previously shown that SOCS-3 antagonizes regulation cellular events cAMP is expressed in human prostate cancer. To investigate possible effects androgen on protein expression, two cancer cell lines (PC3-AR LAPC4) were treated with different concentrations R1881. Western blot analyses revealed induction expression both androgen, an effect...
Fibroblast growth factor-2 (FGF-2) is highly expressed in prostate cancer. It promotes tumour progression through multiple pathways including those of signal transducers and activators transcription factor 3 (STAT3), mitogen-activated protein kinases (MAPKs) Akt. In previous studies, we have reported that STAT3 phosphorylation inversely correlates with suppressor cytokine signalling-3 (SOCS-3) expression cancer cells. Recently, it has become evident SOCS-3-negative regulation not only...
// Martin Puhr 1 , Julia Hoefer Hannes Neuwirt 2 Iris E. Eder Johann Kern 3 Georg Schäfer Stephan Geley 4 Isabel Heidegger Helmut Klocker Zoran Culig Experimental Urology, Department of Medical University Innsbruck, Austria Internal Medicine IV (Nephrology and Hypertension), Oncotyrol Laboratory for Tumor Biology Angiogenesis, Division Molecular Pathophysiology, Innsbruck Biocenter, Correspondence to: Puhr, e-mail: martin.puhr@i-med.ac.at Culig, zoran.culig@i-med.ac.at Keywords:...