A5004662244- Prostate Cancer Treatment and Research
- Cancer, Lipids, and Metabolism
- Cancer Cells and Metastasis
- Computational Drug Discovery Methods
- Cancer-related Molecular Pathways
- Cancer Genomics and Diagnostics
- Prostate Cancer Diagnosis and Treatment
- Cancer, Hypoxia, and Metabolism
- Estrogen and related hormone effects
- Wnt/β-catenin signaling in development and cancer
- PARP inhibition in cancer therapy
- Ubiquitin and proteasome pathways
- Radiopharmaceutical Chemistry and Applications
- Cancer-related gene regulation
- Breast Cancer Treatment Studies
- Epigenetics and DNA Methylation
- Metastasis and carcinoma case studies
- Telomeres, Telomerase, and Senescence
- DNA Repair Mechanisms
- Cancer-related molecular mechanisms research
- Peroxisome Proliferator-Activated Receptors
- Histone Deacetylase Inhibitors Research
- Proteoglycans and glycosaminoglycans research
- Cell Adhesion Molecules Research
- Cancer Treatment and Pharmacology
University Hospital Olomouc
2011-2025
Palacký University Olomouc
2016-2025
Institute of Molecular and Translational Medicine
2016-2025
Vitkovice - Research and Development (Czechia)
2024
Czech Academy of Sciences, Institute of Biophysics
2024
Hudson Institute
2019
John Wiley & Sons (United States)
2019
Faculty (United Kingdom)
2012-2013
Innsbruck Medical University
2010-2011
Johns Hopkins University
2011
Abstract Background Invasive ductal and lobular carcinomas (IDC ILC) are the most common histological types of breast cancer. Clinical follow-up data metastatic patterns suggest that development progression these tumors different. The aim our study was to identify gene expression profiles IDC ILC in relation normal epithelial cells. Methods We examined 30 samples (normal cells from 10 patients, 5 patients) microdissected cryosections ten mastectomy specimens postmenopausal patients. Fifty...
Inhibitors of histone deacetylases have been approved for clinical application in cancer treatment. On the other hand, acetyltransferase (HAT) inhibitors less extensively investigated their potential use therapy. In prostate cancer, HATs and coactivators p300 CBP are upregulated may induce transcription androgen receptor (AR)-responsive genes, even absence or presence low levels AR. To discover a anticancer effect p300/CBP inhibition, we used two different approaches: (i) downregulation by...
Impaired DNA damage response pathways may create vulnerabilities of cancer cells that can be exploited therapeutically. One such selective vulnerability is the sensitivity BRCA1- or BRCA2-defective tumors (hence defective in repair by homologous recombination, HR) to inhibitors poly(ADP-ribose) polymerase-1 (PARP-1), an enzyme critical for alternative HR. While promising, treatment with PARP-1 (PARP-1i) faces some hurdles, including (1) acquired resistance, (2) search other sensitizing,...
Abstract Background Androgen receptor targeted therapies have emerged as an effective tool to manage advanced prostate cancer (PCa). Nevertheless, frequent occurrence of therapy resistance represents a major challenge in the clinical management patients, also because molecular mechanisms behind are not yet fully understood. In present study, we therefore aimed identify novel targets intervene with using gene expression analysis PCa co-culture spheroids where cells grown presence...
Prostate cancer is the most frequently diagnosed malignant tumour in men worldwide. To treat this condition, prognostic markers to distinguish indolent from aggressive disease, and biomarkers for metastatic forms are needed. From a pathologist's perspective, despite plethora of emerging biomarkers, none date has made its way into clinical practice. The need predictive following histological evaluation remains. This overview some putative immunohistochemical genetic reveals pitfalls biomarker...
Collagen triple helix repeat containing 1 (CTHRC1) affects Wnt signalling, collagen deposition and bone formation. It is an extracellular matrix protein which also abnormally expressed in the tumour microenvironment. CTHRC1 has not been studied breast cancer by immunohistochemistry.To examine expression of together with periostin versican patients investigate its association clinicopathological characteristics.The formalin-fixed paraffin-embedded tissues 173 invasive carcinomas (classified...
Although the induction of senescence in cancer cells is a potent mechanism tumor suppression, senescent remain metabolically active and may secrete broad spectrum factors that promote tumorigenicity neighboring malignant cells. Here we show androgen deprivation therapy (ADT), widely used treatment for advanced prostate cancer, induces senescence-associated secretory phenotype epithelial cells, indicated by increases β-galactosidase activity, heterochromatin protein 1β foci, expression...
Abstract The identification of fibroblasts and cancer‐associated from human cancer tissue using surface markers is difficult, especially because the used currently are usually not expressed solely by fibroblasts, fibroblast‐specific molecules still under investigation. It was aimed to compare three commercially available antibodies in detection different epitopes (anti‐fibroblast, fibroblast activation protein α, protein). specificity their expression, employing cell lines tumor‐derived...
Autophagy is an evolutionarily conserved process that captures aberrant intracellular proteins and/or damaged organelles for delivery to lysosomes, with implications cellular and organismal homeostasis, aging diverse pathologies, including cancer. During cancer development, autophagy may play both tumour-supporting tumour-suppressing roles. Any relationships of the established oncogene-induced replication stress (RS) ensuing DNA damage response (DDR)-mediated anti-cancer barrier in early...
Background: The aim of this study was to analyse the usefulness detecting important apoptosis and proliferation markers in assessing biological potential odontogenic keratocysts (OKC) thus selecting optimal diagnostic algorithm for these lesions. Methods: Indirect immunohistochemistry relevant statistical methods were used analysis formalin‐fixed paraffin‐embedded samples from 98 patients. Results: Nevoid basal cell carcinoma syndrome (NBCCS) characterized by higher expression Bcl‐2, p27...
The DNA damage checkpoints provide an anti-cancer barrier in diverse tumour types, however this concept has remained unexplored prostate cancer (CaP). Furthermore, targeting repair defects by PARP1 inhibitors (PARPi) as a treatment strategy is emerging yet requires suitable predictive biomarkers. To address these issues, we performed immunohistochemical analysis of multiple markers signalling, oxidative stress, and cell cycle control pathways during progression human disease from benign...
Small
The development of colon cancer, one the most common malignancies, is accompanied with numerous lipid alterations. However, analyses whole tumor samples may not always provide an accurate description specific changes occurring directly in epithelial cells. Here, we analyzed detail phospholipid (PL), lysophospholipid (lysoPL), and fatty acid (FA) profiles purified EpCAM+ cells, isolated from adjacent non-tumor tissues cancer patients. We found that a number FAs increased significantly which...
Glioblastoma multiforme is the most common malignant brain tumor in adults, and it among lethal of all cancers. Recent studies have shown that ligand activation peroxisome proliferator-activated receptor (PPAR)-γ can induce differentiation inhibit proliferation several cancer cells. In this study, we investigated whether one PPARγ particular, ciglitazone, inhibits cell viability and, additionally, affects cycle apoptosis human glioblastoma lines T98G, U-87 MG, A172, U-118 MG. All were found...
We provide a detailed characteristic of stem cells isolated and expanded from the human dental pulp. Dental pulp express mesenchymal cell markers STRO-1, vimentin, CD29, CD44, CD73, CD90, CD166, Sox2, nestin, nucleostemin. They are multipotent as shown by their osteogenic chondrogenic potential. measured relative telomere length in 11 lines at different passages quantitative real-time PCR. Despite large proliferative capacity, stable viability, phenotype, genotype over prolonged cultivation,...
Abstract BACKGROUND Steroid receptor coactivators p300 and CBP are highly expressed in advanced prostate cancer. They potentiate activation of androgen by androgens anti‐androgens. In the present study, we have addressed question whether these enhance activity estrogen receptor‐beta (ER‐β), which is variably cancers. METHODS Expression levels were manipulated plasmid or siRNA transfections ER‐β was measured luciferase assays. Viability MTT assays cellular migration determined wound‐healing...
Abstract Triple negative breast cancers (TNBC) are a morphologically and genetically heterogeneous group of with uncertain prediction biological behavior response to therapy. Epithelial mesenchymal transition (EMT) is dynamic process characterized by loss typical epithelial phenotype acquisition characteristics. Aberrant activation EMT can aggravate the prognosis patients cancer, however, mechanisms role microRNAs (miRNAs) in still unclear. The aim our study was analyze miRNA expression...