A5071449865- Renal Transplantation Outcomes and Treatments
- Animal Virus Infections Studies
- Clinical Nutrition and Gastroenterology
- Virus-based gene therapy research
- Viral Infections and Immunology Research
- Organ Transplantation Techniques and Outcomes
- Complement system in diseases
- Renal Diseases and Glomerulopathies
- Polyomavirus and related diseases
- Organ and Tissue Transplantation Research
- Parvovirus B19 Infection Studies
- Transplantation: Methods and Outcomes
- Metabolism and Genetic Disorders
- Virology and Viral Diseases
- Cytomegalovirus and herpesvirus research
- SARS-CoV-2 and COVID-19 Research
- Organ Donation and Transplantation
- Blood groups and transfusion
- Renal and Vascular Pathologies
- Intestinal Malrotation and Obstruction Disorders
- Pregnancy and Medication Impact
- Energy Harvesting in Wireless Networks
- Monoclonal and Polyclonal Antibodies Research
- Systemic Sclerosis and Related Diseases
- Vasculitis and related conditions
Medical University of Vienna
2016-2025
Children's Hospital of Pittsburgh
2004-2017
Universitat Autònoma de Barcelona
2017
FWF Austrian Science Fund
2016
St Thomas' Hospital
2014
King's College London
2014
UPMC Montefiore
2004
University of Pittsburgh
1999-2004
Export Late antibody-mediated rejection (ABMR) is a leading cause of kidney allograft failure. Uncontrolled studies have suggested efficacy the proteasome inhibitor bortezomib, but no systematic trial has been undertaken to support its use in ABMR. In this randomized, placebo-controlled (the Bortezomib Antibody-Mediated Kidney Transplant Rejection [BORTEJECT] Trial), we investigated whether two cycles bortezomib (each cycle: 1.3 mg/m2 intravenously on days 1, 4, 8, and 11) prevent GFR...
Fewer than 50% of kidney transplant recipients (KTRs) develop antibodies against the SARS-CoV-2 spike protein after 2 doses an mRNA vaccine. Preliminary data suggest that a heterologous vaccination, combining and viral vector vaccines, may increase immunogenicity.To assess effectiveness third dose vs vaccine in KTRs who did not have vaccine.This was single center, single-blinded, 1:1 randomized clinical trial SARS-CoV-2, conducted from June 15 to August 16, 2021, 201 had developed Data...
Late antibody-mediated rejection (ABMR) is a leading cause of transplant failure. Blocking IL-6 has been proposed as promising therapeutic strategy.
Drug-induced immunosuppression following kidney transplantation is crucial to prevent allograft rejection, but increases risk for infectious disease. Tailoring of drug dosing both rejection and infection greatly desirable. The apathogenic ubiquitous torque teno virus (TTV) reflects immunocompetence the host might be a potential candidate immunologic monitoring. To assess TTV as an biomarker, load was prospectively quantified in peripheral blood 169 consecutive renal recipients at Medical...
Luminex-based anti-HLA IgG detection on single-antigen flow beads (SAFB) represents a valuable tool for characterization of allosensitization patterns. Assay interpretation, however, may be impeded by false-low test results caused the prozone effect. Recent experimental data have related this artifact to direct blockade complement component C1 as possible candidate mechanism.To dissect causative role complex formation and subsequent steps classical activation, native or modified sera...
Antibody-mediated rejection (AMR) represents one of the cardinal causes late allograft loss after kidney transplantation, and there is great need for noninvasive tools improving early diagnosis this type. One promising strategy might be quantification peripheral blood DNA levels highly prevalent apathogenic Torque Teno virus (TTV), which mirror overall level immunosuppression thus help determine risk alloimmune response.To assess association between TTV load in AMR, 715 transplant recipients...
The nonpathogenic and ubiquitous torque teno virus (TTV) is associated with immunosuppression in solid organ transplant recipients. Studies kidney patients proposed TTV quantification for risk stratification of graft rejection infection. In this prospective trial (DRKS00012335) 386 consecutive recipients were subjected to longitudinal per-protocol monitoring plasma load by polymerase chain reaction 12 months posttransplant. peaked at the end month 3 posttransplant reached steady state...
Background. Late antibody-mediated rejection (AMR) is a major cause of transplant failure. Potential therapeutic targets are plasma cells and natural killer (NK) cells, both expressing high levels CD38. Methods. Here, we report the use CD38 monoclonal antibody daratumumab (9-mo course) in kidney allograft recipient diagnosed with smoldering myeloma anti-HLA class II donor-specific antibody-positive chronic active AMR 13 years after transplantation. Patient monitoring included serial HLA...
Potent immunosuppressive drugs have been introduced into clinical care for solid organ transplant recipients. It is now time to guide these on an individual level optimize their efficacy. An ideal tool simultaneously detects overimmunosuppression and underimmunosuppression, highly standardized, straightforward implement routine. Randomized controlled interventional trials are crucial demonstrate value. To date, proposed assays mainly focused the prediction of rejection were based assessment...
Abstract Background Immunosuppression after kidney transplantation is mainly guided via plasma tacrolimus trough level, which cannot sufficiently predict allograft rejection and infection. The load of the non-pathogenic highly prevalent torque teno virus (TTV) associated with immunosuppression its host. Non-interventional studies suggest use TTV to primary objective current trial demonstrate safety, tolerability preliminary efficacy TTV-guided immunosuppression. Methods For this purpose, a...
Torque Teno virus (TTV) is nonpathogenic, highly prevalent, and reflects the immune status of its host. Thus, TTV plasma load was suggested for guidance immunosuppression post solid organ transplantation. The present study designed to determine kinetics following changes in calcineurin inhibitor (CNI) dose. A total 48 adult recipients a kidney graft transplanted at Medical University Vienna between 2018 2019 with isolated CNI dose were selected from prospective TTV-POET trial. quantified by...
Circulating donor-specific antibodies (DSA) detected on bead arrays may not inevitably indicate ongoing antibody-mediated rejection (AMR). Here, we investigated whether detection of complement-fixation, in parallel to IgG mean fluorescence intensity (MFI), allows for improved prediction AMR.Our study included 86 DSA+ kidney transplant recipients subjected protocol biopsy, who were identified upon cross-sectional antibody screening 741 with stable graft function at 6 months or longer after...
Apheresis-based desensitization allows for successful transplantation across major immunological barriers. For donor-specific antibody (DSA)- and/or crossmatch-positive transplantation, however, it has been shown that even intense immunomodulation may not completely prevent antibody-mediated rejection (ABMR).In this study, we evaluated transplant outcomes in 101 DSA+ deceased donor kidney recipients (transplantation between 2009 and 2013; median follow-up: 24 months) who were subjected to...
Drug-induced immunosuppression in kidney transplant recipients is crucial to prevent allograft rejection, but increases risk for infectious disease. Immunologic monitoring tailor immunosuppressive drugs might alloreactivity and adverse effects simultaneously. The apathogenic torque teno virus (TTV) reflects the immunocompetence of its host act as a potential candidate holistic monitoring. We screened all 1010 consecutive patients from prospective Vienna Kidney Transplant Cohort Study...
Late antibody-mediated rejection (ABMR) triggered by donor-specific antibodies (DSA) is a cardinal cause of kidney allograft dysfunction and loss. Diagnostic criteria for this type are well established, but effective treatment remains major challenge. Recent randomized controlled trials (RCT) have failed to demonstrate the efficacy widely used therapies, such as rituximab plus intravenous immunoglobulin or proteasome inhibition (bortezomib), reinforcing great need new therapeutic concepts....
Nonpathogenic torque teno viruses (TTVs) are highly prevalent in transplant recipients and associated with immunosuppression. Studies kidney patients have proposed assessment of TTV load for risk stratification clinically overt graft rejection. The value quantification the context subclinical rejection has not been evaluated.In this prospective trial, 307 consecutive were subjected to per-protocol monitoring plasma TTV. was analyzed protocol biopsies (n = 82), scheduled 1 year...
Introduction Kidney transplant recipients (KTR) are at high risk of developing severe COVID-19. However, vaccine response in this population is severely impaired with humoral rates 36–54 and 55–69% after two or three doses SARS-COV-2 vaccines, respectively. Triple immunosuppression specifically the use anti-proliferative agents such as mycophenolic acid (MPA) azathioprine (AZA) have been identified factors for hypo-responsiveness. Methods We hypothesized that non-responders to least previous...
Torque Teno virus (TTV) is non-pathogenic, highly prevalent and reflects the immune status of its host. TTV plasma load was suggested for risk stratification graft rejection infection post kidney-transplantation, which most studies applied an in-house PCR. Recently, a commercial PCR CE-certified clinical use. The present study designed to assess performance as quantified by in prediction infection. Patients events were selected from prospective TTV-POET trial, including 683 consecutive adult...
Immunosuppressed individuals such as kidney transplant recipients (KTR) and hemodialysis patients (DP) show impaired immune responses to COVID-19 vaccination. Plasma Torque Teno Virus (TTV) DNA load is used surrogate for the individual degree of immunosuppression. We now assessed association TTV at time vaccination with humoral cellular response rates in KTR, DP, healthy medical personnel (MP). A total 100 115 DP 54 MP were included. All SARS-CoV-2 seronegative either BNT162b2 or mRNA-1273....
Despite major advances in transplant medicine, improvements long-term kidney allograft survival have not been commensurate with those observed shortly after transplantation. The formation of donor-specific antibodies (DSA) and ongoing antibody-mediated rejection (AMR) processes may critically contribute to late graft loss. However, appropriate treatment for AMR has yet defined. There is accumulating evidence that the proteasome inhibitor bortezomib substantially affect function integrity...
Background and objectives Recent studies highlighting a role of C4d− antibody-mediated rejection (ABMR) have debated whether C4d staining has independent value as marker. Considering the presumed complement an important effector graft injury, this study hypothesized that capillary C4d, footprint antibody-triggered activation, indicates particularly severe manifestation ABMR. Design, setting, participants, & measurements This large retrospective clinicopathologic sought to assess clinical...
Abstract Background Chronic deterioration of kidney graft function is related to inadequate immunosuppression (IS). A novel tool assess the individual net state IS in transplanted patients might be monitoring Torque teno virus (TTV) viral load. TTV a non-pathogen detectable almost all individuals. level peripheral blood has been linked immune-competence its host and should thus reflect after solid organ transplantation. Methods plasma load was quantified monthly by RT-PCR for period 1 year...