A5020412340- Cytomegalovirus and herpesvirus research
- Animal Virus Infections Studies
- Herpesvirus Infections and Treatments
- Viral Infections and Immunology Research
- SARS-CoV-2 detection and testing
- Virus-based gene therapy research
- Toxoplasma gondii Research Studies
- SARS-CoV-2 and COVID-19 Research
- Polyomavirus and related diseases
- Biosensors and Analytical Detection
- Parvovirus B19 Infection Studies
- Transplantation: Methods and Outcomes
- Immune Cell Function and Interaction
- Viral-associated cancers and disorders
- Advanced biosensing and bioanalysis techniques
- Respiratory viral infections research
- Neonatal Health and Biochemistry
- Virology and Viral Diseases
- Plant Virus Research Studies
- HIV Research and Treatment
- Molecular Biology Techniques and Applications
- Genomics and Chromatin Dynamics
- Mosquito-borne diseases and control
- Mycobacterium research and diagnosis
- Lymphoma Diagnosis and Treatment
Medical University of Vienna
2015-2024
Agency for Quality Assurance and Accreditation Austria
2021
University of Vienna
2001-2008
Vienna Biocenter
2001
Drug-induced immunosuppression following kidney transplantation is crucial to prevent allograft rejection, but increases risk for infectious disease. Tailoring of drug dosing both rejection and infection greatly desirable. The apathogenic ubiquitous torque teno virus (TTV) reflects immunocompetence the host might be a potential candidate immunologic monitoring. To assess TTV as an biomarker, load was prospectively quantified in peripheral blood 169 consecutive renal recipients at Medical...
Background The human Torque Teno virus (TTV) causes persistent viremia in most immunocompetent individuals. Elevated TTV levels are observed after solid organ transplantation and related to the extent of immunosuppression especially during phase maintenance immunosuppression. However, which increase early post-transplantation is associated with patient's immunosuppressive state unclear. Objectives In this study, we assessed dynamics detail first three months lung under a defined regimen...
Antibody-mediated rejection (AMR) represents one of the cardinal causes late allograft loss after kidney transplantation, and there is great need for noninvasive tools improving early diagnosis this type. One promising strategy might be quantification peripheral blood DNA levels highly prevalent apathogenic Torque Teno virus (TTV), which mirror overall level immunosuppression thus help determine risk alloimmune response.To assess association between TTV load in AMR, 715 transplant recipients...
The nonpathogenic and ubiquitous torque teno virus (TTV) is associated with immunosuppression in solid organ transplant recipients. Studies kidney patients proposed TTV quantification for risk stratification of graft rejection infection. In this prospective trial (DRKS00012335) 386 consecutive recipients were subjected to longitudinal per-protocol monitoring plasma load by polymerase chain reaction 12 months posttransplant. peaked at the end month 3 posttransplant reached steady state...
Potent immunosuppressive drugs have been introduced into clinical care for solid organ transplant recipients. It is now time to guide these on an individual level optimize their efficacy. An ideal tool simultaneously detects overimmunosuppression and underimmunosuppression, highly standardized, straightforward implement routine. Randomized controlled interventional trials are crucial demonstrate value. To date, proposed assays mainly focused the prediction of rejection were based assessment...
Torque Teno virus (TTV) is nonpathogenic, highly prevalent, and reflects the immune status of its host. Thus, TTV plasma load was suggested for guidance immunosuppression post solid organ transplantation. The present study designed to determine kinetics following changes in calcineurin inhibitor (CNI) dose. A total 48 adult recipients a kidney graft transplanted at Medical University Vienna between 2018 2019 with isolated CNI dose were selected from prospective TTV-POET trial. quantified by...
In lung transplant patients undergoing immunosuppression, more than one human cytomegalovirus (HCMV) genotype may emerge during follow-up, and this could be critical for the outcome of HCMV infection. Up to now, many cases infection with multiple genotypes were probably overlooked due limitations current genotyping approaches. We have now analyzed mixed-genotype infections in 17 clinical samples from 9 using highly sensitive ultradeep-pyrosequencing (UDPS) technology. UDPS was performed at...
Torque teno viruses (TTV) are small DNA-viruses, of the genus Alphatorquevirus, whose replication is linked to immune status. TTV load may be an indicator for efficacy IS in lung transplant recipients (LTRs). In a prospective single-center-study 143 LTRs were followed up and tested by quantitative TTV-DNA PCR. Using multivariate Cox-regression contribution TTV-load occurrence severe infections, chronic allograft dysfunction (CLAD), acute cellular rejection (ACR), death was assessed. During...
Abstract Objectives The qualitative results of SARS-CoV-2 specific real-time reverse transcription (RT) PCR are used for initial diagnosis and follow-up Covid-19 patients asymptomatic virus carriers. However, clinical decision-making health management policies often based additionally on cycle threshold (C t ) values (i.e., quantitative results) to guide patient care, segregation discharge individuals testing positive. Therefore, an analysis inter-protocol variability is needed assess the...
Drug-induced immunosuppression in kidney transplant recipients is crucial to prevent allograft rejection, but increases risk for infectious disease. Immunologic monitoring tailor immunosuppressive drugs might alloreactivity and adverse effects simultaneously. The apathogenic torque teno virus (TTV) reflects the immunocompetence of its host act as a potential candidate holistic monitoring. We screened all 1010 consecutive patients from prospective Vienna Kidney Transplant Cohort Study...
Nonpathogenic torque teno viruses (TTVs) are highly prevalent in transplant recipients and associated with immunosuppression. Studies kidney patients have proposed assessment of TTV load for risk stratification clinically overt graft rejection. The value quantification the context subclinical rejection has not been evaluated.In this prospective trial, 307 consecutive were subjected to per-protocol monitoring plasma TTV. was analyzed protocol biopsies (n = 82), scheduled 1 year...
Broad and decentralised testing of SARS-CoV-2 RNA genomes is a WHO-recommended strategy to contain the pandemic by identifying infected cases in order minimize onward transmission. With need increase test capacities Austria, nation-wide numerous laboratories rapidly implemented assays for molecular detection based on real-time RT-PCR assays. The objective this study was monitor reliability laboratory results through an external quality assessment (EQA) scheme. For this, Center Virology,...
Torque Teno virus (TTV) is non-pathogenic, highly prevalent and reflects the immune status of its host. TTV plasma load was suggested for risk stratification graft rejection infection post kidney-transplantation, which most studies applied an in-house PCR. Recently, a commercial PCR CE-certified clinical use. The present study designed to assess performance as quantified by in prediction infection. Patients events were selected from prospective TTV-POET trial, including 683 consecutive adult...
Immunosuppressed individuals such as kidney transplant recipients (KTR) and hemodialysis patients (DP) show impaired immune responses to COVID-19 vaccination. Plasma Torque Teno Virus (TTV) DNA load is used surrogate for the individual degree of immunosuppression. We now assessed association TTV at time vaccination with humoral cellular response rates in KTR, DP, healthy medical personnel (MP). A total 100 115 DP 54 MP were included. All SARS-CoV-2 seronegative either BNT162b2 or mRNA-1273....
Glycoprotein O (gO) of the human cytomegalovirus (HCMV) is critical subunit envelope trimer gH/gL/gO as it interacts with platelet-derived growth factor alpha receptor upon fibroblast entry, and triggers gB-mediated fusion for epithelial cell infection. Eight genotypes (GT) highly polymorphic gO gene are described, yet unclear whether distinct GTs differ in their function. Thus, we aimed to elucidate potential functional differences between two diverse an otherwise genomically identical HCMV...
Human cytomegalovirus (HCMV) does not exist as one defined virus genotype, but a variety of different strains. Several studies have investigated the significance specific viral genotypes for clinical course HCMV infection. Upon reinfection, patients may acquire additional strains, and infections with mixture strains appear to be quite common. The analysis such mixed has become increasingly important, only investigating implications mixed-genotype infections, also understanding pathogenesis...
Human cytomegalovirus (CMV) is a major cause of disease and transplant dysfunction in lung recipients. Simultaneous emergence more than one CMV-genotype can occur, appears to be disadvantageous for the patient. In this study, dynamics individual CMV-genotypes blood was assessed within mixed populations emerging after transplantation. 69 plasma 76 bronchoalveolar lavage samples 16 recipients with infections first year posttransplantation each glycoprotein B (gB) H (gH) genotypes selectively...
The human virome plays an important role for the clinical outcome of lung transplant recipients (LTRs). While pathogenic viruses may cause severe infections, non-pathogenic serve as potential markers level immunosuppression. However, neither complexity in different compartments nor dynamics virus populations posttransplantation are yet understood. Therefore, this study was analyzed by metagenomic sequencing simultaneously withdrawn bronchoalveolar lavage (BAL) and plasma samples 15 LTRs. In...
IntroductionEarlier reports suggest that patients after ABO-incompatible kidney transplantation (ABOi) are at enhanced risk to develop BK-virus (BKV) nephropathy (BKPyVAN). It remains elusive whether this is a result of more intense immunosuppression or an ABOi-associated 'intrinsic attribute'. To address question, we measured Torque-Teno-Virus (TTV) loads as quantitative proxy for immunosuppressive depth in ABOi recipients and compared them HLA-incompatible (HLAi, i.e. pre-transplant...