A5064913810- Ion channel regulation and function
- Neonatal Respiratory Health Research
- Ion Transport and Channel Regulation
- Cardiac electrophysiology and arrhythmias
- Ion Channels and Receptors
- Barrier Structure and Function Studies
- Neuroscience of respiration and sleep
- Cellular transport and secretion
- Neuroscience and Neural Engineering
- Tracheal and airway disorders
- Lipid Membrane Structure and Behavior
- Erythrocyte Function and Pathophysiology
- Inhalation and Respiratory Drug Delivery
- Electrical and Bioimpedance Tomography
- Microfluidic and Bio-sensing Technologies
- Genetic Neurodegenerative Diseases
- Adenosine and Purinergic Signaling
- Neuroscience and Neuropharmacology Research
- Cystic Fibrosis Research Advances
- 3D Printing in Biomedical Research
- Pluripotent Stem Cells Research
- Cardiomyopathy and Myosin Studies
- Renal function and acid-base balance
- Force Microscopy Techniques and Applications
- Nasal Surgery and Airway Studies
Universität Ulm
2014-2025
Luther University
2010
Martin Luther University Halle-Wittenberg
2010
TU Dresden
2010
Life & Brain (Germany)
2010
Czech Academy of Sciences, Institute of Physiology
2010
Ludwig-Maximilians-Universität München
2010
Institute for Biomedical Engineering
2010
Friedrich Schiller University Jena
2010
Leibniz Institute of Plant Genetics and Crop Plant Research
2010
Background— Ion channels are key determinants for the function of excitable cells, but little is known about their role and involvement during cardiac development. Earlier work identified Ca 2+ -activated potassium small intermediate conductance (SKCas) as important regulators neural stem cell fate. Here we have investigated impact on differentiation pluripotent cells toward lineage. Methods Results— We applied SKCa activator 1-ethyl-2-benzimidazolinone embryonic this particular ion channel...
Ca 2+ is considered a key element in multiple steps during regulated exocytosis. During the postfusion phase, an elevated cytoplasmic concentration ([Ca ]) c leads to fusion pore dilation. In neurons and neuroendocrine cells, this results from activation of voltage-gated channels plasma membrane. However, these are activated prefusion stage, little known about entry mechanisms stage. This may be particularly important for slow nonexcitable secretory cells. We recently described...
Secretion of vesicular contents via exocytosis is a fundamental cellular process. Increasing evidence suggests that post-fusion events play an important role in determining the composition and quantity secretory output. In particular, regulation fusion pore dilation/closure considered key regulator phase. However, dependent on nature cargo additional mechanisms might be essential to facilitate effective release. We have recently described alveolar type II (ATII) cells lamellar bodies (LBs),...
The lung epithelium constitutes a selective barrier that separates the airways from aqueous interstitial compartment. Regulated function controls water and ion transport across is essential for maintaining function. Tight junctions (TJs) seal epithelial determine paracellular transport. properties of TJs depend especially on their claudin composition. Steroids are potent drugs used to treat variety airway diseases. Therefore, we addressed whether steroid hormones directly act TJ in...
Abstract The modulation of cell proliferation in neural progenitor cells (NPCs) is believed to play a role neuronal regeneration. Recent studies showed that K + channel activity influenced proliferation. Therefore, we examined NPCs for channels and tested whether NPC self renewing can be modulated by synthetic modulators. whole‐cell current was partly dependent cumulative inactivating component. Two tetra‐ethyl‐ammonium ion (TEA)‐sensitve currents with different voltage dependencies ( = 65 μ...
Type II pneumocytes secrete surfactant, a lipoprotein‐like substance reducing the surface tension in lung, by regulated exocytosis of secretory vesicles termed lamellar bodies (LBs). This process is characterized protracted postfusion phase which fusion pores open slowly and may act as mechanical barriers for release. Combining dark‐field with fluorescence microscopy, we show ß‐actin green fluorescent protein‐transfected that LB plasma membrane followed actin coating fused LB. inhibited...
Interleukin-13 (IL-13) drives symptoms in asthma with high levels of T-helper type 2 cells (Th2-cells). Since tight junctions (TJ) constitute the epithelial diffusion barrier, we investigated effect IL-13 on TJ human tracheal cells. We observed that increases paracellular permeability, changes claudin expression pattern and induces intracellular aggregation proteins zonlua occludens protein 1, as well claudins. Furthermore, treatment ubiquitin conjugating E2 enzyme UBE2Z. Co-localization...
Patients with an inherited inability to synthesize sufficient amounts of cholesterol develop congenital malformations the skull, toes, kidney and heart. As development these structures depends on functional cilia we investigated whether regulates ciliogenesis through inhibition hydroxymethylglutaryl-Coenzyme A reductase (HMG-CoA-R), rate-limiting enzyme in synthesis. HMG-CoA-R is efficiently inhibited by statins, a standard medication for hyperlipidemia. When zebrafish embryos are treated...
Abstract Ion channels are potent modulators for developmental processes in progenitor cells. In a screening approach different ion neural cells (NPCs) we observed 1‐ethyl‐2‐benzimidazolinone (1‐EBIO) activated inward current, which could be blocked by scyllatoxin (ScTX, IC 50 = 2 ± 0.3 nmol/L). This initial evidence the expression of small conductance Ca 2+ K + ‐channel SK3 was confirmed detection transcripts and protein NPCs. Interestingly, proteins were highly expressed non‐differentiated...
Background Ca2+ is essential for vesicle fusion with the plasma membrane in virtually all types of regulated exocytoses. However, contrast to well-known effects a high cytoplasmic concentration ([Ca2+]c) prefusion phase, occurrence and significance signals postfusion phase have not been described before. Methodology/Principal Findings We studied isolated rat alveolar type II cells using previously developed imaging techniques. These release pulmonary surfactant, complex lipids proteins, from...
We have isolated an hSK3 isoform from a human embryonic cDNA library that we named hSK3_ex4. This contains 15 amino acid insertion within the S5 to P-loop segment. Transcripts encoding hSK3_ex4 are coexpressed at lower levels with in neuronal as well non-neuronal tissues. To investigate pharmacokinetic properties of hSK3_ex4, expressed isoforms and tsA cells. Both were similarly activated by cytosolic Ca<sup>2+</sup> (hSK3, EC<sub>50</sub> = 0.91 ± 0.4 μM; 0.78 0.2 μM)...
Two fundamental mechanisms within alveoli are essential for lung function: regulated fluid transport and secretion of surfactant. Surfactant is secreted via exocytosis lamellar bodies (LBs) in alveolar type II (ATII) cells. We recently reported that LB results fusion-activated cation entry (FACE) P2X4 receptors on LBs. propose FACE, addition to facilitating surfactant secretion, modulates transport. Correlative fluorescence atomic force microscopy revealed FACE-dependent water influx...
Hybrid atomic force microscopy (AFM)-fluorescence (FM) investigation of exocytosis in lung epithelial cells (ATII cells) allows the detection individual exocytic events by FM, which can be simultaneously correlated to structural changes AFM. Exocytosis lamellar bodies (LBs) represents a slow form found many non-neuronal cells. LBs, following stimulation with adenosine-5'-triphosphate (ATP) and phorbol 12-myristate 13-acetate (PMA), results cation influx via P2X(4) receptors at site LB fusion...
Mucus clearance provides an essential innate defense mechanism to keep the airways and lungs free of particles pathogens. Baseline stimulated mucin secretion from secretory airway epithelial cells need be tightly regulated prevent mucus hypersecretion plugging airways. It is well established that extracellular ATP a potent stimulus for secretion. Previous studies revealed acts via metabotropic P2Y 2 purinoreceptors on goblet cells. Extracellular ATP, however, also agonist ionotropic P2X...
Abstract TGF-β1 is a major mediator of airway tissue remodelling during atopic asthma and affects tight junctions (TJs) epithelia. However, its impact on TJs ciliated epithelia sparsely investigated. Herein we elaborated effects primary human bronchial epithelial cells. We demonstrate that activates receptors TGFBR1 TGFBR2 resulting in ALK5-mediated phosphorylation SMAD2. observed -R2 localize specifically motile cilia. activated accumulation phosphorylated SMAD2 (pSMAD2-C) at centrioles...
Lung epithelia regulate the water flux between gas filled airways and interstitial compartment in order to maintain organ function. Current methodology assess transepithelial transport is limited. We present a D2O dilution method quantify submicroliter volumes of aqueous solutions on epithelial cell layers. Evaluating D2O/H2O mixtures using mid-infrared (2–25 μm) attenuated total reflection (ATR) spectroscopy, with resolution 0.06% vol/vol change, corresponding 24 nL, was achieved. Using...
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The pulmonary epithelium separates the gaseous intraluminal space of airways and aqueous interstitium. This compartimentalization is required for appropriate lung function, it established during perinatal periods can be disturbed in edema. Herein we elaborated impact air-liquid interface (ALI) on function epithelium. We used NCI-H441 epithelia as a well-established characterized model distal airway epithelia, which were cultivated either at ALI or (at submerged conditions) liquid-liquid...