A5028019355- Microtubule and mitosis dynamics
- Photosynthetic Processes and Mechanisms
- Advanced Electron Microscopy Techniques and Applications
- Cellular transport and secretion
- Cellular Mechanics and Interactions
- Cardiomyopathy and Myosin Studies
- Electron and X-Ray Spectroscopy Techniques
- Bacterial Genetics and Biotechnology
- Fungal and yeast genetics research
- Alzheimer's disease research and treatments
- Nuclear Structure and Function
- Enzyme Structure and Function
- Genomics and Chromatin Dynamics
- Protist diversity and phylogeny
- Advanced Fluorescence Microscopy Techniques
- RNA and protein synthesis mechanisms
- Ubiquitin and proteasome pathways
- Mitochondrial Function and Pathology
- Lipid Membrane Structure and Behavior
- Micro and Nano Robotics
- Force Microscopy Techniques and Applications
- Skin and Cellular Biology Research
- 14-3-3 protein interactions
- Bacteriophages and microbial interactions
- Proteins in Food Systems
University of Colorado Boulder
2013-2022
University of Colorado System
2010-2019
National and Kapodistrian University of Athens
2013
University of Maryland, Baltimore
2009
Biotechnology Institute
2009
Okayama University
2009
European Molecular Biology Laboratory
1998-2008
Max Planck Unit for Structural Molecular Biology
1998-2008
Ludwig-Maximilians-Universität München
2008
European Molecular Biology Laboratory
1998-2006
We analyzed the structure of yeast endoplasmic reticulum (ER) during six sequential stages budding by electron tomography to reveal a three-dimensional portrait ER organization inheritance at nanometer resolution. have determined distribution, dimensions, and ribosome densities structurally distinct but continuous domains multiple with without tubule-shaping proteins, reticulons (Rtns) Yop1. In wild-type cells, peripheral contains cytoplasmic cisternae, many tubules, large plasma membrane...
Identification of therapeutic strategies to prevent or cure diseases associated with amyloid fibril deposition in tissue (Alzheimer's disease, spongiform encephalopathies, etc.) requires a rational understanding the driving forces involved formation these organized assemblies rich β-sheet structure. To this end, we used computer-designed algorithm search for hexapeptide sequences high propensity form homopolymeric β-sheets. Sequences predicted be highly favorable on basis were found...
Microtubule plus-end-tracking proteins (+TIPs) localize to growing microtubule plus ends regulate a multitude of essential functions. End-binding (EBs) form the core this network by recognizing distinct structural feature transiently existing in an extended region at and recruiting other +TIPs region. The nature conformational difference allowing EBs discriminate between tubulins potential tubulin binding sites farther away from end is unknown. By combining vitro reconstitution, multicolor...
We investigate acute effects of axial stretch, applied by carbon fibers (CFs), on diastolic Ca2+ spark rate in rat isolated cardiomyocytes. CFs were attached either to both cell ends (to maximize the stretched region), or center and one end compare responses nonstretched half-cells). Sarcomere length was increased 8.01+/-0.94% fraction, time series XY confocal images recorded monitor frequency dynamics. Whole-cell stretch causes an increase 130.7+/-6.4%) within 5 seconds, followed a return...
Nucleolar and spindle-associated protein (NuSAP) was recently identified as a microtubule- chromatin-binding in vertebrates that is nuclear during interphase. Small interfering RNA-mediated depletion of NuSAP resulted aberrant spindle formation, missegregation chromosomes, ultimately blocked cell proliferation. We show here enriched on chromatin-proximal microtubules at meiotic spindles Xenopus oocytes. When added higher than physiological levels to egg extract, induces extensive bundling...
Cytokinesis and abscission are complicated events that involve changes in membrane transport cytoskeleton organization. We have used the combination of time-lapse microscopy correlative high-resolution 3D tomography to analyze regulation spatio-temporal remodeling endosomes microtubules during abscission. show is driven by formation a secondary ingression within intracellular bridge connecting two daughter cells. The initiation expansion this requires recycling endosome fusion with furrow...
Defects in flagella growth are related to a number of human diseases. Central flagellar is the organization microtubules that polymerize from basal bodies form axoneme, which consists hundreds proteins. Flagella exist all eukaryotic phyla, but neither mechanism by grow nor conservation this process evolution known. Here, we study how protein complexes assemble onto growing axoneme tip using (cryo) electron tomography. In Chlamydomonas reinhardtii and associated proteins added simultaneously....
We have identified dimeric kinesin mutants that become stalled on the microtubule after one ATP turnover, unable to bind and hydrolyze at their second site. used these determine regulatory signal allows forward head, such processive movement can continue. The results show phosphate release occurs from rearward head before detachment, detachment triggers active-site accessibility for binding head. This mechanism, in which controls behavior of may be conserved among motors.
We have decorated microtubules with monomeric and dimeric kinesin constructs, studied their structure by cryoelectron microscopy three-dimensional image reconstruction, compared the results x-ray crystal of kinesin. A construct (rK354, containing only a short neck helix insufficient for coiled-coil formation) decorates stoichiometry one head per tubulin subunit (α–β-heterodimer). The orientation (an anterograde motor) on microtubule surface is similar to that ncd (a retrograde motor). longer...
Giardia lamblia is a flagellated, unicellular parasite of mammals infecting over one billion people worldwide. Giardia's two-stage life cycle includes motile trophozoite stage that colonizes the host small intestine and an infectious cyst form can persist in environment. Similar to many eukaryotic cells, contains several complex microtubule arrays are involved motility, chromosome segregation, organelle transport, maintenance cell shape transformation between two stages. trophozoites also...