A5023979223- Cell Image Analysis Techniques
- DNA Repair Mechanisms
- Inflammasome and immune disorders
- Epigenetics and DNA Methylation
- Osteomyelitis and Bone Disorders Research
- Zebrafish Biomedical Research Applications
- Photoreceptor and optogenetics research
- Renal and related cancers
- Advanced Fluorescence Microscopy Techniques
- Single-cell and spatial transcriptomics
- Cancer therapeutics and mechanisms
- Retinal Development and Disorders
- Liver Disease Diagnosis and Treatment
- Cancer-related Molecular Pathways
- Bacterial Genetics and Biotechnology
- Spondyloarthritis Studies and Treatments
- Cancer-related gene regulation
- Cell death mechanisms and regulation
- Machine Learning in Materials Science
- Fault Detection and Control Systems
- Hydraulic and Pneumatic Systems
- Advanced Electron Microscopy Techniques and Applications
- Infectious Diseases and Tuberculosis
- Rheumatoid Arthritis Research and Therapies
- DNA and Nucleic Acid Chemistry
Center for Systems Biology Dresden
2019-2024
TU Dresden
2016-2024
A deeper epigenomic understanding of spatial organization cells in human tissues is an important challenge. Here we report the first combined positional analysis transcriptomes and methylomes across three micro-dissected zones (pericentral, intermediate periportal) liver. We identify pronounced anti-correlated transcriptional methylation gradients including a core 271 genes controlling zonated metabolic morphogen networks observe prominent porto-central gradient DNA at binding sites 46...
Germ cell development involves major reprogramming of the epigenome to prime zygote for totipotency. Histone 3 lysine 4 (H3K4) methylations are universal epigenetic marks mediated in mammals by six H3K4 methyltransferases related fly Trithorax, including two yeast Set1 orthologs: Setd1a and Setd1b. Whereas plays no role oogenesis, we report that Setd1b deficiency causes female sterility mice. Oocyte-specific Gdf9-iCre conditional knockout (Setd1bGdf9 cKO) ovaries develop through all stages;...
A modern day light microscope has evolved from a tool devoted to making primarily empirical observations what is now sophisticated , quantitative device that an integral part of both physical and life science research. Nowadays, microscopes are found in nearly every experimental laboratory. However, despite their prevalent use capturing quantifying scientific phenomena, neither thorough understanding the principles underlying imaging techniques nor appropriate knowledge how calibrate,...
Morphogen gradients impart positional information to cells in a homogenous tissue field. Fgf8a, highly conserved growth factor, has been proposed act as morphogen during zebrafish gastrulation. However, technical limitations have so far prevented direct visualization of the endogenous Fgf8a gradient and confirmation its morphogenic activity. Here, we monitor propagation developing neural plate using CRISPR/Cas9-mediated EGFP knock-in at fgf8a locus. By combining sensitive imaging with...
Chronic nonbacterial osteomyelitis (CNO), an autoinflammatory bone disease primarily affecting children, can cause pain, hyperostosis and fractures, quality-of-life psychomotor development. This study investigated CNO-associated variants in P2RX7, encoding for the ATP-dependent trans-membrane K+ channel P2X7, their effects on NLRP3 inflammasome assembly. Whole exome sequencing two related transgenerational CNO patients, target of P2RX7 a large cohort (N = 190) were conducted. Results...
Fluorescence lifetime imaging microscopy (FLIM) translates the duration of excited states fluorophores into information as additional source contrast in images biological samples. This offers possibility to separate particularly beneficial case similar excitation spectra. Here, we demonstrate distinction fluorescent molecules based on FLIM phasor analysis, called unmixing, live-cell using open-source software for analysis. We showcase two applications Caenorhabditis elegans a model system....
The heterotrimeric replication protein A (RPA) has multiple essential activities in eukaryotic DNA metabolism and signaling pathways. Despite extensive analyses, the functions of smallest RPA subunit p14 are still unknown. To solve this issue we produced characterized a dimeric complex lacking p14, RPADeltap14, consisting p70 p32. RPADeltap14 was able to bind single-stranded DNA, but its binding mode affinity differed from those complex. Moreover, p32 only minimally recognized 3'-end primer...
Unlike humans, teleosts like zebrafish exhibit robust retinal regeneration after injury from endogenous stem cells. However, it is unclear if regenerating cone photoreceptors regain physiological function and integrate correctly into post-synaptic circuits. We used two-photon calcium imaging of living adult retina to examine photoreceptor responses before light-induced lesions. To assess functional recovery cones downstream outer circuits, we exploited color opponency; UV intrinsic...
Abstract Morphogen gradients impart positional information to cells in a homogenous tissue field. Fgf8a, highly conserved growth factor, has been proposed act as morphogen during zebrafish gastrulation. However, technical limitations have so far prevented direct visualization of the endogenous Fgf8a gradient and confirmation its morphogenic activity. Here, we monitored propagation developing neural plate using CRISPR/Cas9-mediated EGFP knock-in at fgf8a locus. By combining sensitive imaging...
Replication protein A (RPA) is a stable heterotrimeric complex consisting of p70, p32 and p14 subunits. The plays crucial role in SV40 minichromosome replication. Peptides p70 representing interaction sites for the smaller two subunits, DNA as well viral initiator large T‐antigen (Tag) cellular polymerase α‐primase (Pol) all interfered with replication process indicating importance different activities this process. Inhibition by peptide disrupting protein–protein interactions was observed...
The initiation of SV40 (simian virus 40) DNA replication requires the co-operative interactions between viral Tag (large T-antigen), RPA (replication protein A) and Pol (DNA polymerase α-primase) on template DNA. Binding interfaces mapped these enzymes expressed as peptides competed with mutual native proteins. Prevention genuine was accomplished only prior to primer synthesis step blocked assembly a productive complex. Once complex engaged in an RNA its extension, interfering effects...
<h3>Aims</h3> Chronic Nonbacterial Osteomyelitis (CNO) is an autoinflammatory bone disease primarily affecting children. It can cause pain, hyperostosis and fractures, thereby quality-of-life psychomotor development. While increased inflammasome activation has been reported, the exact molecular pathophysiology of CNO unknown, no biomarkers exist. <h3>Aim</h3> To identify mechanisms in to allow patient stratification individualized treatment. <h3>Methods</h3> Whole exome sequencing families...
Transverse Myelitis und Retrobulbarneuritis bei einer jugendlichen Patientin mit systemischem Lupus erythematodes -
Background: Chronic nonbacterial osteomyelitis (CNO), an autoinflammatory bone disease primarily affecting children, can cause pain, hyperostosis and fractures, quality-of-life psychomotor development. This study investigated CNO-associated variants in P2RX7, encoding for the ATP-dependent trans-membrane K+ channel P2X7, their effects on NLRP3 inflammasome assembly.Methods: Whole exome sequencing two related transgenerational CNO patients, target of P2RX7 a large cohort (N=190) were...
Summary Unlike humans, teleosts like zebrafish exhibit robust retinal regeneration after injury from endogenous stem cells. However, understanding the functional recovery of regenerated retina remains a challenge. In particular, it is unclear if regenerating cone-photoreceptors can regain physiological function and integrate correctly into post-synaptic circuits. To bridge this gap, we employed two-photon calcium imaging living retina, focusing on photoreceptor responses before an intense...
A deeper epigenomic understanding of spatial organization cells in human tissues is an important challenge. Here we report the first combined positional analysis transcriptomes and methylomes across three micro-dissected zones (pericentral, intermediate periportal) liver. We identify pronounced anti-correlated transcriptional methylation gradients including a core 271 genes controlling zonated metabolic morphogen networks observe prominent porto-central gradient DNA at binding sites 46...
Die metabolische Zonierung der Leber und deren Regulation wurde in den letzten Jahrzehnten intensiv erforscht. Das Hauptaugenmerk meist an Tiermodellen durchgeführten Versuche lag dabei vor allem auf dem gesunden Organ. Insbesondere die sogenannten omics-Methoden führten hierbei zu bahnbrechenden Ergebnissen. Trotzdem Leberzirrhose eine wichtigsten Erkrankungen westlicher Länder darstellt, ist es bisher nicht gelungen diesem fortgeschrittenen Stadium unter Nutzung dieser Techniken analysieren.