A5017744793- Inflammasome and immune disorders
- Immune Response and Inflammation
- Immune Cell Function and Interaction
- Cell death mechanisms and regulation
- interferon and immune responses
- Immunotherapy and Immune Responses
- HIV Research and Treatment
- Kawasaki Disease and Coronary Complications
- Malaria Research and Control
- T-cell and B-cell Immunology
- IL-33, ST2, and ILC Pathways
- Innovative Microfluidic and Catalytic Techniques Innovation
- Digestive system and related health
- S100 Proteins and Annexins
- Mosquito-borne diseases and control
- Hepatitis B Virus Studies
- Biomarkers in Disease Mechanisms
- Cancer-related Molecular Pathways
- NF-κB Signaling Pathways
- Organ Transplantation Techniques and Outcomes
- Phagocytosis and Immune Regulation
- Cell Adhesion Molecules Research
- Cancer Cells and Metastasis
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Pancreatic function and diabetes
University of Oxford
2020-2024
Jenner Institute
2020-2023
TU Dresden
2014-2022
University Hospital Carl Gustav Carus
2015-2022
Max Planck Institute for Molecular Genetics
2014
Pro-inflammatory caspase-1 is a key player in innate immunity. Caspase-1 processes interleukin (IL)-1β and IL-18 to their mature forms triggers pyroptosis. These functions are linked its enzymatic activity. However, loss-of-function missense mutations CASP1 do not prevent autoinflammation patients, despite decreased IL-1β production. In vitro data suggest that enzymatically inactive drives inflammation via enhanced nuclear factor κB (NF-κB) activation, independent of processing. Here, we...
Adjuvanted protein vaccines offer high efficacy, yet most potent adjuvants remain proprietary. Several adjuvant compounds are being developed by the Vaccine Formulation Institute in Switzerland for global open access clinical use. In context of R21 malaria vaccine, a mouse challenge model, we characterize efficacy and mechanism action four adjuvants: two liposomal (LQ LMQ) squalene emulsion-based (SQ SMQ), containing QS-21 saponin (Q) optionally synthetic TLR4 agonist (M). Two vaccine...
<title>Abstract</title> Inadequate booster uptake threatens the success of immunisation campaigns as seen with recently rolled-out malaria vaccine. Ability to administer prime-boost a single injection would save lives and alleviate healthcare burdens. We present first platform for delayed delivery dose, that is scalable existing technology, easily injectable highly protective against malaria. Using chip microfluidics, we encapsulated R21 vaccine into polymer microcapsules release their...
Immunological processes that underpin the administration of therapeutics and vaccines are poorly defined due to a lack models which faithfully recapitulate human immune responses. Inbred mice diversity inherent people, while microanatomical organisation tissue is lost in isolated cell suspensions. We describe precision-cut lymph node (LN) slices as architecturally-preserved, functioning lymphoid model system, explore early inflammatory responses potent vaccine liposomal adjuvant containing...
LPS is frequently used to induce experimental endotoxic shock, representing a standard model of acute inflammation in mice. The resulting inflammatory response leads hypothermia the animals, which turn can be as surrogate for severity systemic inflammation. Although increasingly applied humane endpoint murine studies, differences between obtained temperature-time curves are typically evaluated at single time point with t -tests or ANOVA analyses. We hypothesized that analyses entire using...
Allogeneic transplantation of human islets improves metabolic control in patients that suffer from type 1 diabetes but is limited by the supply sufficient numbers donor islets. Therefore, xenogenic porcine potentially represents an attractive alternative. The immediate blood‐mediated inflammatory reaction (IBMIR) results early graft rejection one major hurdles preventing xenotransplantation clinical applications. Thus, characterizing and modulating IBMIR could be key to realize islet...
The proinflammatory enzyme caspase-1 belongs to the family of cystein proteases and plays a pivotal role in innate immunity. Classically, mediated signaling is thought be dependent on its enzymatic activity. Interestingly, number patients carrying variants CASP1 gene show symptoms like febrile episodes despite reduced activity protein resulting decreased IL-1β production. In vitro investigations this contradictory phenomenon revealed receptor interacting kinase 2 (RIP2) as potential mediator...
Caspase-1, belonging to the family of cystein proteases, is well-known for its involvement in IL-1β and IL-18 maturation. Although, caspase-1 one best described caspase-family-members, open questions concerning autoinflammatory diseases, caused by procaspase-1 variants (e.g. ICE-Fever), remain. ICE-Fever patients suffer from chronic febrile episodes, although show reduced enzymatic activity leading limited secretion. The paradox secretion but increased inflammation led hypothesis, that...
<title>Abstract</title> Incomplete vaccination poses a significant challenge to worldwide disease eradication efforts, resulting in approximately 1.5 million preventable deaths each year<sup>1</sup>. A single-injection method for administering full prime-boost course of immunisation would help improve global coverage, reduce deaths, and alleviate logistical cost burdens on healthcare systems<sup>2,3</sup>. To address this need, we have developed system high-precision continuous production...
Time after LPS injection Body temperature (°C) 0 h 6 1 2 8 4 25 30 35 40 B6 WT Casp1 KO C284A PBS