A5024588035- Pancreatic function and diabetes
- Diabetes and associated disorders
- Diabetes Management and Research
- Xenotransplantation and immune response
- Cannabis and Cannabinoid Research
- Pluripotent Stem Cells Research
- Hormonal Regulation and Hypertension
- COVID-19 Clinical Research Studies
- Genetic Associations and Epidemiology
- Tissue Engineering and Regenerative Medicine
- Pancreatitis Pathology and Treatment
- Pancreatic and Hepatic Oncology Research
- Orthodontics and Dentofacial Orthopedics
- Genetics and Neurodevelopmental Disorders
- dental development and anomalies
- 3D Printing in Biomedical Research
- Virus-based gene therapy research
- Metabolism, Diabetes, and Cancer
- Immune Cell Function and Interaction
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Genetic and phenotypic traits in livestock
- Diet, Metabolism, and Disease
- Receptor Mechanisms and Signaling
- Polyomavirus and related diseases
- Craniofacial Disorders and Treatments
Paul Langerhans Institute Dresden
2015-2024
University Hospital Carl Gustav Carus
2015-2024
TU Dresden
2015-2024
Deutsches Diabetes-Zentrum e.V.
2014-2024
German Center for Diabetes Research
2013-2024
Heinrich Heine University Düsseldorf
2014-2024
Helmholtz Zentrum München
2015-2024
Center for Environmental Health
2023-2024
King Abdullah University of Science and Technology
2023
Center for Systems Biology Dresden
2023
Significance Diabetes mellitus type 1 is an autoimmune disease that results in irreversible destruction of insulin-producing beta cells. Substantial advances have been made cell replacement therapies over the last decades. However, lack eligible donor organs and need for chronic immunosuppression to prevent rejection critically limit a widespread application these strategies. In this paper we present clinical success using bioartificial pancreas transplantation islets without affecting...
Abstract Metabolic diseases are associated with an increased risk of severe COVID-19 and conversely, new-onset hyperglycemia complications preexisting diabetes have been observed in patients. Here, we performed a comprehensive analysis pancreatic autopsy tissue from patients using immunofluorescence, immunohistochemistry, RNA scope electron microscopy detected SARS-CoV-2 viral infiltration beta-cells all Using pseudoviruses, confirmed that isolated human islet cells permissive to infection....
The current epidemic of diabetes with its overwhelming burden on our healthcare system requires better therapeutic strategies. Here we present a promising novel approach for curative strategy that may be accessible all insulin-dependent patients. We designed subcutaneous implantable bioartificial pancreas (BAP)-the "β-Air"-that is able to overcome critical challenges in clinical islet transplantation protocols: adequate oxygen supply the graft and protection donor islets against host immune...
Islet transplantation is a feasible therapeutic alternative for metabolically labile patients with type 1 diabetes. The primary target stable glycemic control and prevention of complications associated diabetes by reconstitution endogenous insulin secretion. However, critical shortage donor organs, gradual loss in graft function over time, chronic need immunosuppression limit the indication islet to small group patients. Here we present promising approach address these limitations...
Developing a device that protects xenogeneic islets to allow treatment and potentially cure of diabetes in large mammals has been major challenge the past decade. Using for transplantation is required light donor shortage number diabetic patients qualify islet transplantation. Until now, however, host immunoreactivity against graft drawback use porcine islets. Our study demonstrates applicability novel immunoprotective membrane allows successful xenotransplantation rat minipigs without...
Transplantation of pancreatic islets is a promising strategy to alleviate the unstable blood-glucose control that some patients with diabetes type 1 exhibit and has seen many advances over years. Protection transplanted from immune system can be accomplished by encapsulation within hydrogel, most investigated which alginate. In this study, islet combined 3D extrusion bioprinting, an additive manufacturing method enables fabrication structures precise geometry produce macroporous hydrogel...
Significance Diabetes mellitus type 1 is an autoimmune disease that results in irreversible destruction of insulin-producing beta cells. Substantial advances have been made cell replacement therapies during the last decades. However, lack eligible donor organs and need for chronic immunosuppression to prevent rejection critically limit widespread application these strategies. In this manuscript, we present experimental study using a bioartificial pancreas device transplantation xenogeneic...
Abstract Obese adipose tissue (AT) inflammation contributes critically to development of insulin resistance. The complement anaphylatoxin C5a receptor (C5aR) has been implicated in inflammatory processes and as regulator macrophage activation polarization. However, the role C5aR obesity AT not addressed. We engaged model diet-induced found that expression was significantly upregulated obese AT, compared with lean AT. In addition, present proximity macrophage-rich crownlike structures....
Islet transplantation as a biological β-cell replacement therapy has emerged promising option for achieving restoration of metabolic control in type 1 diabetes patients. However, partial or complete loss islet graft function occurs relatively short time (months to few years) after implantation. The high rate early transplant dysfunction been attributed poorly viable and/or functional islets and is mediated by innate inflammatory response at the intravascular (hepatic) site critical lack...
Islet-cell hormone release is modulated by signals from endothelial and endocrine cells within the islet. However, models of intraislet vascularization paracrine cell signaling are mostly based on rodent pancreas. We assessed architecture interaction vascular network in unperturbed human islets situ their potential to re-establish endogenous after transplantation vivo. prepared slices fresh pancreas tissue obtained nondiabetic patients undergoing partial pancreatectomy. In addition, we...
At the time first pancreas transplant was performed by Kelly and Lillehei in 1966, insulin therapy for diabetes generally available but administered a form that is known today as “conventional therapy.”1 In this era, many half of all juvenile onset diabetics did not reach age 55 years. Early mortality from accelerated cardiovascular disease, renal failure, hypoglycemia-related events were commonplace. The early low success rate transplantation comparison also suboptimal. As will be...
Significance Adrenal insufficiency is a life-threatening disorder that requires complex and permanent hormone replacement strategy. All current schemes suffer from numerous problems, as they fail to restore circadian variations in secretion. Therefore, adrenal cell transplantation could be preferable therapeutic alternative for patients suffering primary dysfunction. This strategy critically limited, however, by the lack of suitable donors human organs requirement chronic immunosuppression....
Transplantation of encapsulated islets can cure diabetes without immunosuppression, but oxygen supply limitations cause failure. We investigated a retrievable macroencapsulation device wherein are in planar alginate slab and supplied with exogenous from replenishable gas chamber. Translation to clinically-useful devices entails reduction size by increasing islet surface density, which requires increased chamber pO
Pancreatic islets of Langerhans secrete hormones to regulate systemic glucose levels. Emerging evidence suggests that islet cells are functionally heterogeneous allow a fine-tuned and efficient endocrine response physiological changes. A precise description the molecular basis this heterogeneity, in particular linking animal models human islets, is an important step towards identifying factors critical for cell function pathophysiological conditions. In study, we used single-cell RNA...