A5070853764- Cellular Mechanics and Interactions
- Cancer Cells and Metastasis
- 3D Printing in Biomedical Research
- Connexins and lens biology
- Monoclonal and Polyclonal Antibodies Research
- Ion channel regulation and function
- Cell Adhesion Molecules Research
- CAR-T cell therapy research
- Cancer Research and Treatments
- Heme Oxygenase-1 and Carbon Monoxide
- Laser Applications in Dentistry and Medicine
- Phagocytosis and Immune Regulation
- Ion Channels and Receptors
- Neonatal Respiratory Health Research
- Advanced Biosensing Techniques and Applications
- Angiogenesis and VEGF in Cancer
- Glycosylation and Glycoproteins Research
- Microtubule and mitosis dynamics
- Hippo pathway signaling and YAP/TAZ
- Epigenetics and DNA Methylation
- ATP Synthase and ATPases Research
- Adenosine and Purinergic Signaling
- Asthma and respiratory diseases
- Immunotherapy and Immune Responses
- RNA Interference and Gene Delivery
Radboud University Nijmegen
2009-2025
Radboud University Medical Center
2009-2025
University Medical Center
2017-2025
Radboud Institute for Molecular Life Sciences
2009-2022
Inserm
2012
Centre de Recherche Cardio-Thoracique de Bordeaux
2012
Université de Bordeaux
2012
University of Würzburg
2009
Siberian Institute of Plant Physiology and Biochemistry
2008
Cancer invasion into an extracellular matrix (ECM) results from a biophysical reciprocal interplay between the expanding cancer lesion and tissue barriers imposed by adjacent microenvironment. In vivo, connective provides both densely packed ECM to channel/track-like spaces loosely organized zones, of which may impact mode efficiency; however little is known about how three-dimensional (3D) aligned tracks present in interstitial guide cell invasion. We here describe two-photon laser ablation...
Breast cancer undergoes collective tissue invasion and, in experimental models, can collectively metastasize. The prevalence of and its contribution to distant metastasis clinical disease, however, remains poorly defined. We here scored the adipose primary invasive ductal carcinoma (IDC), expressing E-cadherin, E-cadherin negative lobular (ILC) identified predominantly patterns (86/86 samples) both types. Whereas IDC lesions retained adherens junctions, multicellular clusters "Indian files"...
Cancer invasion programs are adaptive by switching between metastatic collective and single-cell dissemination; however, current intravital microscopy models for epithelial cancer in mice fail to reliably recreate such plasticity. Using microimplantation of breast spheroids into the murine mammary fat pad live-cell monitoring, we show microenvironmental conditions cytoskeletal adaptation during transition vivo E-cadherin-expressing 4T1 E-cadherin-negative MMT tumors both initiated along...
Abstract In breast cancer (BC), radial alignment of collagen fibers at the tumor-matrix interface facilitates collective invasion cells into surrounding stromal matrix, a critical step toward metastasis. Collagen remodeling is driven by proteases and cellular forces, mediated matrix mechanical plasticity, or irreversible deformation in response to force. However, specific mechanisms causing remain unclear. Here, we study BC tumor spheroids collagen-rich matrices. Increasing plasticity...
Progression of epithelial cancers predominantly proceeds by collective invasion cell groups with coordinated cell-cell junctions and multicellular cytoskeletal activity. Collectively invading breast cancer cells express the gap junction protein connexin-43 (Cx43), yet whether Cx43 regulates remains unclear. We here show that mediates gap-junctional coupling between collectively and, via hemichannels, adenosine nucleotide/nucleoside release into extracellular space. Using molecular...
The antitumor immunity can be enhanced through the synchronized codelivery of antigens and immunostimulatory adjuvants to antigen-presenting cells, particularly dendritic cells (DCs), using nanovaccines (NVs). To study influence intracellular vaccine cargo release kinetics on T cell activating capacities DCs, we compared stimuli-responsive nonresponsive polymersome NVs. do so, employed "AND gate" multiresponsive (MR) amphiphilic block copolymers that decompose only in response combination...
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Abstract Background While immune checkpoint inhibitors such as anti-PD-L1 antibodies have revolutionized cancer treatment, only subgroups of patients show durable responses. Insight in the relation between clinical response, PD-L1 expression and intratumoral localization therapeutics could improve patient stratification. Therefore, we present modular synthesis multimodal antibody-based imaging tools for multiscale to study distribution therapeutics. Results To introduce modalities, a peptide...
Abstract Progression of epithelial cancers predominantly proceeds by collective invasion cell groups with coordinated cell-cell junctions and multicellular cytoskeletal activity. Collectively invading breast cancer cells co-express adherens connexin-43 (Cx43) gap in vitro patient samples, yet whether contribute to remains unclear. We here show that Cx43 is required for chemical coupling between collectively and, its hemichannel function, adenosine nucleotide release into the extracellular...