A5056322825- Cellular Mechanics and Interactions
- Cancer Cells and Metastasis
- Connexins and lens biology
- Hippo pathway signaling and YAP/TAZ
- Melanoma and MAPK Pathways
- Myasthenia Gravis and Thymoma
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Lung Cancer Treatments and Mutations
- Lung Cancer Diagnosis and Treatment
- Lymphatic System and Diseases
- Pulmonary Hypertension Research and Treatments
- Cell death mechanisms and regulation
- Advanced Radiotherapy Techniques
- Wnt/β-catenin signaling in development and cancer
- Glaucoma and retinal disorders
- Cell Adhesion Molecules Research
- Radiation Effects and Dosimetry
- PARP inhibition in cancer therapy
- Cancer-related Molecular Pathways
- Respiratory and Cough-Related Research
- COVID-19 Clinical Research Studies
- Pneumonia and Respiratory Infections
- Lymphatic Disorders and Treatments
- Ocular Oncology and Treatments
- Inhalation and Respiratory Drug Delivery
University Medical Center Utrecht
2019-2025
Hôpital Bichat-Claude-Bernard
2017-2024
Aarhus University Hospital
2024
Inserm
2022-2024
Assistance Publique – Hôpitaux de Paris
2017-2024
Université Paris Cité
2017-2024
Université Claude Bernard Lyon 1
2017-2024
Hospices Civils de Lyon
2024
Centre Hospitalier Universitaire de Nantes
2024
Foundation for Human Potential
2023
Abstract The tumor micro-environment often contains stiff and irregular-bundled collagen fibers that are used by cells to disseminate. It is still unclear how what extent, extracellular matrix (ECM) stiffness versus ECM bundle size alignment dictate cancer cell invasion. Here, we have uncoupled Collagen-I bundling from introducing inter-collagen crosslinks, combined with temperature induced aggregation of bundling. Using organotypic models mouse invasive ductal lobular breast cancers, show...
The extracellular matrix (ECM) is essential for cell support during homeostasis and plays a critical role in cancer. Although research often concentrates on the tumor’s cellular aspect, attention growing importance of cancer-associated ECM. Biochemical physical ECM signals affect tumor formation, invasion, metastasis, therapy resistance. Examining microenvironment uncovers intricate dysregulation interactions with cancer stromal cells. Anticancer therapies targeting sensors remodelers,...
The purpose of this study was to evaluate the efficacy and safety selective arterial embolization control severe postpartum hemorrhage.Twenty-five women with intractable hemorrhage underwent uterine in our institution during a 6-year period.Angiography revealed extravasation 13 patients (52%). Sixty-nine arteries were embolized. External bleeding resolved immediately or markedly decreased 24 women. In one patient, failed bleeding, surgical treatment required. No major complication therapy...
Abstract Dense and aligned Collagen I fibers are associated with collective cancer invasion led by protrusive tumor cells, leader cells. In some breast tumors, a population of cells (basal-like cells) maintain several epithelial characteristics express the myoepithelial/basal cell marker Keratin 14 (K14). Emergence K14 expression regarded as interconnected events triggered I, however underlying mechanisms remain unknown. Using carcinoma organoids, we show that drives force-dependent loop,...
Breast cancer undergoes collective tissue invasion and, in experimental models, can collectively metastasize. The prevalence of and its contribution to distant metastasis clinical disease, however, remains poorly defined. We here scored the adipose primary invasive ductal carcinoma (IDC), expressing E-cadherin, E-cadherin negative lobular (ILC) identified predominantly patterns (86/86 samples) both types. Whereas IDC lesions retained adherens junctions, multicellular clusters "Indian files"...
Background Development of diffuse-type gastric cancer (DGC) starts with intramucosal lesions that are primarily composed differentiated, non-proliferative signet ring cells (SRCs). These indolent can advance into highly proliferative and metastatic tumours, which requires suppression DGC cell differentiation. Objective Our goal was to identify molecular changes contributing the progression aggressive lesions. Design We conducted spatial transcriptomic analysis patient tumours at different...
Collective invasion is the predominant mode of cancer cell dissemination in breast and represents initial step metastatic spread. Basal-like leader cells drive this process by maintaining cell-cell junctions with follower while extending actin-rich protrusions remodelling collagen I-rich peritumoral stroma. These features resemble those individually-invading following epithelial-to-mesenchymal transition (EMT), however, how acquire these traits preserving cohesion within collective remains...
Progression of epithelial cancers predominantly proceeds by collective invasion cell groups with coordinated cell-cell junctions and multicellular cytoskeletal activity. Collectively invading breast cancer cells express the gap junction protein connexin-43 (Cx43), yet whether Cx43 regulates remains unclear. We here show that mediates gap-junctional coupling between collectively and, via hemichannels, adenosine nucleotide/nucleoside release into extracellular space. Using molecular...
Abstract In breast cancer the transcription factor SOX4 has been shown to be associated with poor survival, increased tumor size and metastasis formation. This mostly attributed ability of regulate Epithelial-to-Mesenchymal-Transition (EMT). However, regulates target gene in a context-dependent manner that is determined by cellular epigenetic state. this study we have investigated loss mammary development utilizing organoids derived from PyMT genetic mouse model cancer. Using CRISPR/Cas9...
Dose-escalation in lung cancer comes with a high risk of severe toxicity. This study aimed to calculate the delivered dose Scandinavian phase-III dose-escalation trial.
Abstract Cancer-derived organoids and three-dimensional (3D) extracellular matrix (ECM) are taking center stage as in vitro models to study neoplastic cell behavior, since they recapitulate the heterogeneous cellular composition of tumors their environment. In combination with imaging molecular/biochemical techniques, 3D organoid have contributed substantially our knowledge about molecular mechanisms that regulate growth invasion into surrounding tissue. We here outline a set protocols...
Crosstalk between gap junction intracellular communication (GJIC), STAT5 and OCT-1 in (GJ)-dependent β-casein expression was investigated. CID-9 mammary cells plated with prolactin on non-adherent substratum (poly-HEMA) expressed independent of only the presence GJIC inducer, cAMP. Nuclear levels were not detectable. By contrast, EHS-drip a STAT5-dependent manner nuclear up-regulated. A 75 kDa isoform detected conditions that induced regardless substratum. Interestingly, 40 28 isoforms...
<title>Abstract</title> <bold>Background:</bold>PARP inhibitors (PARPi) are used in the treatment of ovarian, breast, pancreatic, and prostate cancers. Pneumonitis has been identified as a potential side effect, with higher meta-analysis-assessed risk for olaparib versus other PARPi. Olaparib-induced interstitial lung disease (O-ILD) was first described within Japanese population, few information available Caucasian patients. <bold>Methods:</bold> We performed retrospective study by pooling...
Abstract Colorectal cancer (CRC) cells exhibit high plasticity and transition between different cellular states during the development of metastasis. Lgr5-expressing stem fuel growth primary tumor metastasis, yet disseminated arriving at metastatic site are devoid Lgr5 expression. It is currently unknown how CRC cell fate transitions regulated process give rise to lesions despite being neg . Here, we show that reprogramming disseminating driven by mechanical interactions with Collagen I-rich...