A5039461625- Cancer Cells and Metastasis
- Cellular Mechanics and Interactions
- Molecular Biology Techniques and Applications
- Cancer-related Molecular Pathways
- Hippo pathway signaling and YAP/TAZ
- Bone health and treatments
- RNA modifications and cancer
- Oral and Maxillofacial Pathology
- Bone Metabolism and Diseases
- Wnt/β-catenin signaling in development and cancer
- RNA Interference and Gene Delivery
- Cell death mechanisms and regulation
- Fibroblast Growth Factor Research
- DNA Repair Mechanisms
- PI3K/AKT/mTOR signaling in cancer
- Ear and Head Tumors
- Neurological diseases and metabolism
- Polyamine Metabolism and Applications
- Lung Cancer Treatments and Mutations
- Microbial metabolism and enzyme function
- Angiogenesis and VEGF in Cancer
- Skin and Cellular Biology Research
- Virus-based gene therapy research
University Medical Center Utrecht
2022-2025
Massachusetts General Hospital
2023
Harvard University
2023
Hubrecht Institute for Developmental Biology and Stem Cell Research
2023
Maastricht University Medical Centre
2019
Abstract The tumor micro-environment often contains stiff and irregular-bundled collagen fibers that are used by cells to disseminate. It is still unclear how what extent, extracellular matrix (ECM) stiffness versus ECM bundle size alignment dictate cancer cell invasion. Here, we have uncoupled Collagen-I bundling from introducing inter-collagen crosslinks, combined with temperature induced aggregation of bundling. Using organotypic models mouse invasive ductal lobular breast cancers, show...
Abstract Afadin is a multimodal scaffolding protein with essential functions in cell–cell adhesion. Although its loss of expression has been linked to breast cancer invasion and metastasis, the underlying mechanisms driving tumour progression upon mutational ( AFDN ) cancers remains unclear. In current study we identified somatic frameshift mutation p .Lys630fs) an invasive sample that coincides expression. Functional studies E‐cadherin‐expressing cells show leads immature aberrant adherens...
Cidofovir (CDV) is an antiviral agent with antiproliferative properties. The aim of our study was to investigate the efficacy CDV in HPV-positive and -negative head neck squamous cell carcinoma (HNSCC) lines whether it caused by a difference response DNA damage. Upon treatment HNSCC normal oral keratinocyte lines, we carried out MTT analysis (cell viability), flow cytometry cycle analysis), (immuno) fluorescence western blotting (DNA double strand breaks, damage response, apoptosis mitotic...
Clinical outcome for patients suffering from HPV-negative head and neck squamous cell carcinoma (HNSCC) remains poor. This is mostly due to highly invasive tumors that cause loco-regional relapses after initial therapeutic intervention metastatic outgrowth. The molecular pathways governing the detrimental growth modes in HNSCC remain however understudied. Here, we have established patient derived organoid (PDO) models recapitulate 3-dimensional invasion vitro. Single mRNA sequencing was...
Survival rates of head and neck squamous cell carcinoma (HNSCC) have only marginally improved in the last decades. Hence there is a need for predictive biomarkers long-time survival that can help to guide treatment decisions might lead development new therapies. The phosphatidylinositol 3-kinase (PI3K)/AKT/mTOR signaling pathway most frequently altered HNSCC, genes are often mutated, amplificated overexpressed causing aberrant affecting growth differentiation. Numerous genetic alterations...
Keratin proteins are intermediate filaments that provide mechanical support and guide a plethora of important functions in epithelial cells. Although Keratins can be effectively used to characterize differentiate cell lineages many epithelia, it is still unclear if combinations types their spatial expression patterns aid prognostication head neck squamous carcinoma (HNSCC). Here, we have assessed panel markers four HPV negative HNSCC patient-derived organoid (PDO) models corresponding...
Oral squamous cell carcinoma (OSCC) frequently invades the jaw. The exact mechanism of bone invasion remains unclear. This study investigates (premature) osteoclasts and expression its differentiation regulating proteins RANKL, OPG RANK in patients with OSCC.Resection specimens from OSCC were divided into NI group (No Invasion), E (Erosion) or I (bone Invasion). Tissue sections stained Cathepsin K (osteoclast-counting), RANK. staining intensity was scored on different regions tumor: front,...
Tissue-specific inactivation of E-cadherin combined with tumor suppressor loss leads to invasive and metastatic cancers in mice. While epidermal mice induces squamous cell carcinomas, the mammary gland lobular carcinoma. To further explore carcinogenic consequences cell-cell adhesion these compartments, we developed a new conditional mouse model inactivating (Cdh1) p53 (Trp53) simultaneously cells expressing leucine-rich repeat-containing G-protein coupled receptor 6 (Lgr6), putative...
Abstract Background Oral squamous cell carcinoma (OSCC) frequently invades the jaw. The exact mechanism of bone invasion remains unclear. This study investigates role osteoclasts and RANKL/OPG/RANK in development OSCC. Methods OSCC-patients treated with resection were included divided three groups; Non-invasion (NI-group), erosion (E-group) (I-group). Tissue-sections stained Cathepsin K (for counting osteoclasts), RANKL, OPG RANK. Staining intensity was scored tumor-front, tumor-center,...
<title>Abstract</title> Clinical outcome for patients suffering from head and neck squamous cell carcinoma (HNSCC) remains poor. This is mostly due to highly invasive tumors that cause loco-regional relapses after initial therapeutic intervention. The molecular pathways governing the detrimental growth modes in HNSCC remain however understudied. Here, we have established patient derived organoid (PDO) models recapitulate 3-dimensional invasion <italic>in vitro</italic>. Single mRNA...
Objectives: Cidofovir (CDV) is an antiviral agent with anti-proliferative properties and suggested as treatment option in head neck squamous cell carcinoma (HNSCC) patients to improve outcome quality of life. The mechanisms underlying the effectivity CDV are not completely understood. aim our study was investigate efficacy HPV-positive -negative HNSCC lines vitro whether it caused by a difference response DNA damage.Materials methods: Upon HNSCC-, uterine cervical (UCC)- immortalized NOK-...
Abstract Objectives: Cidofovir (CDV) is an antiviral agent with anti-proliferative properties and suggested as treatment option in head neck squamous cell carcinoma (HNSCC) patients to improve outcome quality of life. The mechanisms underlying the effectivity CDV are not completely understood. aim our study was investigate efficacy HPV-positive -negative HNSCC lines vitro whether it caused by a difference response DNA damage. Materials methods: Upon HNSCC-, uterine cervical (UCC)-...