A5007854650- Monoclonal and Polyclonal Antibodies Research
- CAR-T cell therapy research
- CRISPR and Genetic Engineering
- Cancer Immunotherapy and Biomarkers
- Glycosylation and Glycoproteins Research
- Medical Imaging Techniques and Applications
- Immune Cell Function and Interaction
- Radiopharmaceutical Chemistry and Applications
- Reproductive System and Pregnancy
- Immune cells in cancer
- Advanced Biosensing Techniques and Applications
University Medical Center
2019-2024
Radboud University Nijmegen
2019-2024
Radboud University Medical Center
2019-2024
Radboud Institute for Molecular Life Sciences
2019-2022
Hybridoma technology is instrumental for the development of novel antibody therapeutics and diagnostics. Recent preclinical clinical studies highlight importance isotype therapeutic efficacy. However, since sequence encoding constant domains fixed, tuning function in hybridomas has been restricted. Here, we demonstrate a versatile CRISPR/HDR platform to rapidly engineer immunoglobulin obtain recombinant hybridomas, which secrete antibodies preferred format, species, isotype. Using this...
Abstract Background While immune checkpoint inhibitors such as anti-PD-L1 antibodies have revolutionized cancer treatment, only subgroups of patients show durable responses. Insight in the relation between clinical response, PD-L1 expression and intratumoral localization therapeutics could improve patient stratification. Therefore, we present modular synthesis multimodal antibody-based imaging tools for multiscale to study distribution therapeutics. Results To introduce modalities, a peptide...
Natural killer (NK) cell therapy represents an attractive immunotherapy approach against recurrent epithelial ovarian cancer (EOC), as EOC is sensitive to NK cell-mediated cytotoxicity. However, antitumor activity dampened by suppressive factors in patient ascites. Here, we integrated functional assays, soluble factor analysis, high-dimensional flow cytometry cellular component data and clinical parameters of advanced patients study the mechanisms ascites-induced inhibition cells. Using a...
Abstract Many immunotherapies focus on (re)invigorating CD8 + T cell anti-cancer responses and different nuclear imaging techniques have been developed to measure distributions. In vivo labeling approaches using radiotracers primarily show distributions, while ex labeled cells can migration patterns, homing, tumor infiltration. Currently, a comprehensive head-to-head comparison of in ex-vivo with respect their normal tissue targeting properties correlation the presence is lacking, yet...
Abstract Hybridoma technology is instrumental for the development of novel antibody therapeutics and diagnostics. Recent preclinical clinical studies highlight importance isotype therapeutic efficacy. However, since sequence encoding constant domains fixed, tuning function in hybridomas has been restricted. Here, we demonstrate a versatile CRISPR/HDR platform to rapidly engineer immunoglobulin obtain recombinant which secrete antibodies preferred format, species isotype. Using this platform,...