A5014885640- Ion Channels and Receptors
- Neurobiology and Insect Physiology Research
- Ion channel regulation and function
- Pain Mechanisms and Treatments
- Biochemical Analysis and Sensing Techniques
- Neuroscience of respiration and sleep
- Neuroscience and Neuropharmacology Research
- Neuropeptides and Animal Physiology
- Olfactory and Sensory Function Studies
- Neuroendocrine regulation and behavior
- Thermoregulation and physiological responses
- Herbal Medicine Research Studies
- Ocular Surface and Contact Lens
- Hearing, Cochlea, Tinnitus, Genetics
- Circadian rhythm and melatonin
- Nitric Oxide and Endothelin Effects
- Phytochemicals and Antioxidant Activities
- Neural dynamics and brain function
- Innovative Energy Harvesting Technologies
- Erythrocyte Function and Pathophysiology
- Nicotinic Acetylcholine Receptors Study
- Photoreceptor and optogenetics research
- Cardiac electrophysiology and arrhythmias
- Connexins and lens biology
- Postharvest Quality and Shelf Life Management
Universitat de Miguel Hernández d'Elx
2008-2024
Instituto de Neurociencias
2013-2022
Consejo Superior de Investigaciones Científicas
2002-2020
Instituto de Biologia Molecular e Celular
2011
Scripps Research Institute
2008
Universitat de València
2002
KU Leuven
1994-1998
University of Washington
1987-1997
Universidade do Porto
1991
Gram-negative bacterial infections are accompanied by inflammation and somatic or visceral pain. These symptoms generally attributed to sensitization of nociceptors inflammatory mediators released immune cells. Nociceptor during occurs through activation the Toll-like receptor 4 (TLR4) signalling pathway lipopolysaccharide (LPS), a toxic by-product lysis. Here we show that LPS exerts fast, membrane delimited, excitatory actions via TRPA1, transient potential cation channel is critical for...
1. The role of multiple potassium conductances in action potential repolarization and repetitive firing behavior hypoglossal motoneurons was investigated using intracellular recording techniques a brain stem slice preparation the neonatal rat (0-15 days old). 2. followed by two distinct afterhyperpolarizations (AHPs). early one short duration is termed fAHP; later AHP longer mAHP. amplitudes both AHPs were enhanced membrane depolarization (further from EK). In addition, their reduced high...
Vanilloid receptor subunit 1 (VR1) appears to play a critical role in the transduction of noxious chemical and thermal stimuli by sensory nerve endings peripheral tissues. Thus, VR1 antagonists are useful compounds unravel contribution this pain perception, as well induce analgesia. We have used combinatorial approach identify new, nonpeptidic channel blockers VR1. Screening library trimers N-alkylglycines resulted identification two molecules referred DD161515...
Molecular determinants of threshold differences among cold thermoreceptors are unknown. Here we show that such correlate with the relative expression I KD , a current dependent on Shaker -like Kv1 channels acts as an excitability brake, and TRPM8 cold-activated excitatory current. Neurons responding to small temperature changes have high functional (transient receptor potential cation channel, subfamily M, member 8) low . In contrast, neurons activated by lower temperatures prominent...
Cold thermoreceptors have been described in different territories of the vagus nerve. Application cold temperature to these visceral afferents can evoke major protective reflexes and thermoregulatory responses. However, virtually nothing is known about transduction mechanisms underlying sensitivity vagal afferents. Here, we investigated effects stimulation on intracellular calcium responses excitability cultured sensory neurons rat nodose ganglion. A large fraction were activated by cold,...
Transient receptor potential melastatin 8 (TRPM8) is the best molecular candidate for innocuous cold detection by peripheral thermoreceptor terminals. To dissect out contribution of this cold- and menthol-gated, nonselective cation channel to transduction, we identified BCTC [ N -(4- tert -butylphenyl)-4-(3-chloropyridin-2-yl)piperazine-1-carboxamide] as a potent full blocker recombinant TRPM8 channels. In cold-sensitive trigeminal ganglion neurons mice guinea pig, responses menthol were...
Abstract Specific peripheral sensory neurons respond to increases in extracellular osmolality but the mechanism responsible for excitation is unknown. Here we show that small excite isolated mouse dorsal root ganglion (DRG) and trigeminal (TG) expressing cold-sensitive TRPM8 channel (transient receptor potential channel, subfamily M, member 8). Hyperosmotic responses were abolished by antagonists, absent DRG TG from Trpm8 −/− mice. Heterologously expressed was activated increased around...
From mouse to primate, there is a striking discontinuity in our current understanding of the neural coding motion direction. In non-primate mammals, directionally selective cell types and circuits are signature feature retina, situated at earliest stage visual process. primates, by contrast, direction selectivity hallmark processing areas cortex, but has not been found despite significant effort. Here we combined functional recordings light-evoked responses connectomic reconstruction...
1. Single-electrode voltage clamp recordings in a rat brain stem slice preparation were used to determine the characteristics and postnatal development of hyperpolarization-activated inward current (Ih) hypoglossal motoneurons (HMs). 2. In young adult HMs (> P21), noninactivating, time- voltage-dependent was evident during hyperpolarizing steps membrane potentials negative approximately -65 mV from depolarized holding [Vh = -56.2 +/- 1.0 (SE) mV]. The averaged reversal potential (Erev)...
Transient receptor potential (TRP) channels mediate a wide array of sensory functions. We investigated the role TRPC5, poorly characterized channel widely expressed in central and peripheral nervous system, as osmosensory protein. Here we show that hypoosmotic stimulation activates TRPC5 resulting large calcium influx. The response to osmotically induced membrane stretch is blocked by GsMTx-4, an inhibitor activated ion channels. Direct activation independent phospholipase C function....
Two-pore-domain K + channels provide neuronal background currents that establish resting membrane potential and input resistance; their modulation provides a prevalent mechanism for regulating cellular excitability. The so-called TASK channel subunits (TASK-1 TASK-3) are widely expressed, they robustly inhibited by receptors signal through Gαq family proteins. Here, we manipulated G protein expression phosphatidylinositol 4,5-bisphosphate (PIP 2 ) levels in intact cell-free systems to...
1. The role of calcium conductances in action potential generation and repetitive firing behavior hypoglossal motoneurons (HMs) was investigated using intracellular recording patch-clamp techniques a brain stem slice preparation neonatal rats (0-15 days old). 2. followed by an afterdepolarization (ADP). ADP voltage dependent, increasing with membrane hyperpolarization. Raising the extracellular Ca2+ concentration or replacing Ba2+ increased amplitude, whereas replacement Mn2+ blocked it....
Transient receptor potential channels are a family of cation involved in diverse cellular functions. Most these expressed the nervous system and play key role sensory physiology. TRPM8 (transient melastatine 8), member this family, is activated by cold, cooling substances such menthol icilin voltage. Although thermosensitive channel highly cold neurons, mechanisms underlying its temperature sensitivity still poorly understood. Here we show that, localized cholesterol-rich specialized...
TRPM8, a member of the melastatin subfamily transient receptor potential (TRP) cation channels, is activated by voltage, low temperatures and cooling compounds. These properties its restricted expression to small sensory neurons have made it ion channel with most advocated role in cold transduction. Recent work suggests that activation TRPM8 menthol takes place through shifts voltage-activation curve, which cause open at physiological membrane potentials. By contrast, little known about...
Clotrimazole (CLT) is a widely used drug for the topical treatment of yeast infections skin, vagina, and mouth. Common side effects CLT application include irritation burning pain skin mucous membranes. Here, we provide evidence that transient receptor potential (TRP) channels in primary sensory neurons underlie these unwanted CLT. We found clinically relevant concentrations activate heterologously expressed TRPV1 TRPA1, two TRP act as receptors irritant chemical and/or thermal stimuli...
Animals sense cold ambient temperatures through the activation of peripheral thermoreceptors that express TRPM8, a cold- and menthol-activated ion channel. These receptors can discriminate very wide range from innocuous to noxious. The molecular mechanism responsible for variable sensitivity individual temperature is unclear. To address this question, we performed detailed channel expression analysis cold-sensitive neurons, combining bacterial artificial chromosome (BAC) transgenesis with...