A5067818069- Pain Mechanisms and Treatments
- Ion Channels and Receptors
- Neurobiology and Insect Physiology Research
- Ion channel regulation and function
- Biochemical Analysis and Sensing Techniques
- Exercise and Physiological Responses
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Neuroscience and Neuropharmacology Research
- Musculoskeletal pain and rehabilitation
- Thermoregulation and physiological responses
- Anesthesia and Sedative Agents
- Pharmacological Receptor Mechanisms and Effects
- Neuroscience and Neural Engineering
- Ocular Surface and Contact Lens
- Pain Management and Treatment
- Cancer Treatment and Pharmacology
- Regulation of Appetite and Obesity
- Coordination Chemistry and Organometallics
- Connexins and lens biology
- Natural product bioactivities and synthesis
- Postharvest Quality and Shelf Life Management
- Intraoperative Neuromonitoring and Anesthetic Effects
- Phytochemistry and Biological Activities
- Phytochemicals and Antioxidant Activities
- Neurotransmitter Receptor Influence on Behavior
King's College London
2013-2023
University Hospital of Zurich
2011-2013
University of Zurich
2010-2012
Institute of Social and Preventive Medicine
2012
UCL Australia
2011
University College London
2006
Fibromyalgia syndrome (FMS) is characterized by widespread pain and tenderness, patients typically experience fatigue emotional distress. The etiology pathophysiology of fibromyalgia are not fully explained there no effective drug treatments. Here we show that IgG from FMS produced sensory hypersensitivity sensitizing nociceptive neurons. Mice treated with displayed increased sensitivity to noxious mechanical cold stimulation, fibers in skin-nerve preparations mice an responsiveness...
TRP vanilloid 2 (TRPV2) is a nonselective cation channel expressed prominently in medium- to large-diameter sensory neurons that can be activated by extreme heat (>52°C). These features suggest TRPV2 might transducer of noxious vivo . also hypoosmolarity or cell stretch, suggesting potential roles mechanotransduction. To address the physiological functions somatosensation, we generated knock-out mice and examined their behavioral electrophysiological responses mechanical stimuli. showed...
Abstract Specific peripheral sensory neurons respond to increases in extracellular osmolality but the mechanism responsible for excitation is unknown. Here we show that small excite isolated mouse dorsal root ganglion (DRG) and trigeminal (TG) expressing cold-sensitive TRPM8 channel (transient receptor potential channel, subfamily M, member 8). Hyperosmotic responses were abolished by antagonists, absent DRG TG from Trpm8 −/− mice. Heterologously expressed was activated increased around...
Diabetic neuropathy is a severe complication of long-standing diabetes and one the major etiologies neuropathic pain. Diabetes associated with an increased formation reactive oxygen species electrophilic dicarbonyl compound methylglyoxal (MG). Here we show that MG stimulates heterologously expressed TRPA1 in CHO cells natively MDCK DRG neurons. evokes [Ca(2+)]i-responses expressing neurons but without effect cultured from Trpa1(-/-) mice. Consistent direct, intracellular action,...
Summary Parthenolide, a major constituent of feverfew, acts as partial agonist TRPA1. Parthenolide’s ability to target TRPA1 could explain its therapeutic effects on migraine. Although feverfew has been used for centuries treat pain and headaches is recommended migraine treatment, the mechanism protective action remains unknown. Migraine triggered by calcitonin gene-related peptide (CGRP) release from trigeminal neurons. Peptidergic sensory neurons express series transient receptor potential...
Abstract Complex regional pain syndrome (CRPS) is a posttraumatic condition with an incompletely understood pathophysiological basis. Here, we have examined the cellular basis of in CRPS using behavioral and electrophysiological methods mice treated IgG from patients, combination paw incision. Mice were subjected to hind skin–muscle incision alone, or administration purified either healthy control subjects patients persistent CRPS. Nociceptive function was behaviorally vivo,...
Contrasting findings have been published regarding the role of magnesium sulphate used as an additive to local anaesthetics in peripheral nerve blocks.To clarify effect on excitability.C and Aβ compound action potentials were recorded extracellularly vitro saphenous nerves from adult rats.Saphenous (n = 30) male Wistar rats 19), 12 16 weeks old.Primary sensory afferents tested with a computerised threshold tracking program (QTRAC) supramaximal 1 ms current pulse either alone or after 300...
Diabetic neuropathy is a severe complication of long-standing diabetes and one the major etiologies neuropathic pain.Diabetes associated with an increased formation reactive oxygen species electrophilic dicarbonyl compound methylglyoxal (MG).Here we show that MG stimulates heterologously expressed TRPA1 in CHO cells natively MDCK DRG neurons.MG evokes [Ca 2+ ] i -responses expressing neurons but without effect cultured from Trpa1 2/2 mice.Consistent direct, intracellular action,...
The mechanisms responsible for painful and insensate diabetic neuropathy are not completely understood. Here, we have investigated sensory in the Ins2+/Akita mouse, a hereditary model of diabetes. Akita mice become soon after weaning, show that this is accompanied by an impaired mechanical thermal nociception significant loss intraepidermal nerve fibers. Electrophysiological investigations skin-nerve preparations identified reduced rate action potential discharge mechanonociceptors compared...
SUMMARY Fibromyalgia syndrome (FMS) is a chronic pain condition characterized by widespread and tenderness 1,2 . The etiology pathophysiology of fibromyalgia are unknown there no effective treatments. Here we show that sensory hypersensitivity in FMS caused autoantibodies act sensitizing nociceptive neurons. Administration IgG from patients increased mouse sensitivities to stimulation with mechanical pressure cold. In contrast, transfer depleted samples or healthy control subjects had effect...
The platinum chemotherapeutic oxaliplatin produces dose-limiting pain, dysesthesia, and cold hypersensitivity in most patients immediately after infusion. An improved understanding of the mechanisms underlying these symptoms is urgently required to facilitate development symptomatic or preventative therapies. In this study, we have used skin-saphenous nerve recordings vitro behavioral experiments mice characterize direct effects on different types sensory afferent fibers. Our results...
ABSTRACT TRPA1 expressed in peripheral sensory neurons is important for nociception. Pharmacological inhibition or genetic ablation of profoundly reduces normal behavioural sensitivity to noxious cold and mechanical stimulation, as well neuron responses stimulation. also reverses hypersensitivities chronic pain models vivo . Here we demonstrate that these striking effects inactivation result from an increased constitutive activity kappa opioid receptors (KOR) co-expressed with neurons....