A5037861075- Ion Channels and Receptors
- Laser Applications in Dentistry and Medicine
- Pain Mechanisms and Treatments
- Dermatologic Treatments and Research
- Wound Healing and Treatments
- Neuroscience and Neuropharmacology Research
- Skin Protection and Aging
- Optical Coherence Tomography Applications
- Neuroendocrine regulation and behavior
- Receptor Mechanisms and Signaling
- Neuroscience of respiration and sleep
- Endometriosis Research and Treatment
- Olfactory and Sensory Function Studies
- Phytochemicals and Antioxidant Activities
- Alcohol Consumption and Health Effects
- Photoacoustic and Ultrasonic Imaging
- Herbal Medicine Research Studies
- Thyroid Disorders and Treatments
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Neurobiology and Insect Physiology Research
- Bee Products Chemical Analysis
- Photodynamic Therapy Research Studies
- Pressure Ulcer Prevention and Management
- Neurotransmitter Receptor Influence on Behavior
- Migraine and Headache Studies
University of Florence
2014-2024
University of Siena
2024
Nello Carrara Institute of Applied Physics
2015-2023
National Research Council
2016-2019
University of Pisa
2005
Platinum-based anticancer drugs cause neurotoxicity. In particular, oxaliplatin produces early-developing, painful, and cold-exacerbated paresthesias. However, the mechanism underlying these bothersome dose-limiting adverse effects is unknown. We hypothesized that transient receptor potential ankyrin 1 (TRPA1), a cation channel activated by oxidative stress cold temperature, contributes to mechanical hypersensitivity caused cisplatin. Oxaliplatin cisplatin evoked glutathione-sensitive...
Despite intense investigation, the mechanisms of different forms trigeminal neuropathic pain remain substantially unidentified. The transient receptor potential ankyrin 1 channel (encoded by TRPA1 ) has been reported to contribute allodynia or hyperalgesia in some models, including those produced sciatic nerve constriction. However, role and processes that cause pain-like behaviours from insult are poorly understood. TRPA1, monocytes macrophages, oxidative stress behaviour evoked...
The California bay laurel or Umbellularia californica (Hook. & Arn.) Nutt., is known as the 'headache tree' because inhalation of its vapours can cause severe headache crises. However, underlying mechanism precipitating properties unknown. monoterpene ketone umbellulone, major volatile constituent leaves californica, has irritating properties, and a reactive molecule that rapidly binds thiols. Thus, we hypothesized umbellulone stimulates transient receptor potential ankyrin 1 channel in...
Summary Parthenolide, a major constituent of feverfew, acts as partial agonist TRPA1. Parthenolide’s ability to target TRPA1 could explain its therapeutic effects on migraine. Although feverfew has been used for centuries treat pain and headaches is recommended migraine treatment, the mechanism protective action remains unknown. Migraine triggered by calcitonin gene-related peptide (CGRP) release from trigeminal neurons. Peptidergic sensory neurons express series transient receptor potential...
Ethanol stimulating transient receptor potential vanilloid 1 (TRPV1) on primary sensory neurons promotes neurogenic inflammation, including calcitonin gene-related peptide (CGRP)-mediated coronary dilation. Alcoholic beverages trigger migraine attacks and activation of trigeminal plays a role in migraine. We have investigated guinea pigs whether ethanol by TRPV1 stimulation causes inflammation the trigeminovascular system. Ethanolevoked release neuropeptides from slices dura mater was...
3-Iodothyronamine (T1AM), an endogenous derivative of thyroid hormones, is regarded as a rapid modulator behaviour and metabolism. To determine whether brain hormone levels contribute to these effects, we investigated the effect central administration T1AM on learning pain threshold mice either untreated or pretreated with clorgyline (2.5 mg·kg(-1) , i.p.), inhibitor amine oxidative metabolism.T1AM (0.13, 0.4, 1.32 4 μg·kg(-1) ) vehicle was injected i.c.v. into male mice, after 30 min their...
Ingestion of alcoholic beverages is a known trigger migraine attacks. However, whether and how ethanol exerts its pro-migraine action remains poorly known. Ethanol stimulates the transient receptor potential vanilloid 1 (TRPV1) channel, dehydrogenized metabolite, acetaldehyde, TRP ankyrin (TRPA1) agonist.Periorbital mechanical allodynia following systemic acetaldehyde was investigated in mice after TRPA1 TRPV1 pharmacological antagonism global genetic deletion. Mice with selective silencing...
In our pursuit to identify selective ligands for Bz/GABAA receptor subtypes, a novel pyrazolo[1,5-a]pyrimidine derivative (4), the azaisostere of zolpidem, was synthesized and evaluated in vitro on bovine brain homogenate recombinant benzodiazepine receptors (αxβ2/3γ2, x = 1−3, 5) expressed HEK293 cells. Compound 4 displayed affinity only α1β2γ2 subtype (Ki 31 nM), an in-depth, vivo study it revealed sedative anxiolytic-like properties without any amnesic myorelaxant effects rodents.
BACKGROUND AND PURPOSE Preclinical pharmacology of 3-iodothyronamine (T1AM), an endogenous derivative thyroid hormones, indicates that it is a rapid modulator rodent metabolism and behaviour. Since T1AM undergoes enzymatic degradation, particularly by MAO, we hypothesized the effects might be altered inhibition MAO. EXPERIMENTAL APPROACH We investigated injecting (i.c.v.) on (i) feeding behaviour, hyperglycaemia plasma levels hormones (ii) systemic bioavailability, in overnight fasted mice,...
3-Iodothyroacetic acid (TA1) is an end product of thyroid hormone metabolism. So far, it not known if TA1 present in mouse brain and has any pharmacological effects.TA1 levels were measured by HPLC coupled to mass spectrometry. After i.c.v. administration exogenous (0.4, 1.32 4 μg·kg(-1) ) mice, memory acquisition-retention (passive avoidance paradigm with a light-dark box), pain threshold thermal stimulus (51.5°C; hot plate test) plasma glucose (glucorefractometer) evaluated. Similar assays...
Abstract Background The mechanism underlying the pain symptoms associated with chemotherapeutic-induced peripheral neuropathy (CIPN) is poorly understood. Transient receptor potential ankyrin 1 (TRPA1), TRP vanilloid 4 (TRPV4), TRPV1, and oxidative stress have been implicated in several rodent models of CIPN-evoked allodynia. Thalidomide causes a painful CIPN patients via an unknown mechanism. Surprisingly, pathway responsible for such proalgesic response has not yet investigated animal...
The healing process of superficial skin wounds treated with a blue-LED haemostatic device is studied. Four mechanical abrasions are produced on the back 10 Sprague Dawley rats: two device, while other left to naturally recover. Visual observations, non-linear microscopic imaging, as well histology and immunofluorescence analyses performed 8 days after treatment, demonstrating no adverse reactions neither thermal damages in both abraded areas surrounding tissue. A faster better-recovered...
Background: Recent studies evidence that blue-LED-light irradiation can modulate cell responses in the wound healing process within 24 h from treatment. This study aims to investigate blue-light (410–430 nm) photobiomodulation used a murine model six days post-treatment. Methods: A superficial was made 30 CD1 male mice. The injuries were treated with blue LED light (20.6 J/cm2), and biopsies collected at 24, 72, 144 h. Histology, fluorescence analysis, advanced microscopy techniques used....
Ingestion of elevated amounts ethanol in humans and rodents induces hemorrhagic gastric lesions, at least part by increasing oxidative stress. The present study was undertaken order to evaluate the influence a bicarbonate-alkaline mineral water (Uliveto®) on ethanol-induced lesions mice. Lesions were evaluated both macroscopic microscopic analysis. In first set experiments, mice allowed drink Uliveto® or reference ad libitum until 3 h prior intragastric (i.g.) (23 ml/kg) administration....
Background and Purpose The pro‐algesic transient receptor potential ankyrin 1 (TRPA1) channel, expressed by a subpopulation of primary sensory neurons, has been implicated in various pain models mice. However, evidence rats indicates that TRPA1 conveys nociceptive signals elicited channel activators, but not those associated with tissue inflammation or nerve injury. Here, rats, we explored the role mechanical allodynia stimulation peptidergic neurons (neurogenic inflammation) moderate...
Itch is associated with increased sensitization to nociceptive stimuli. We investigated whether 3-iodothyroacetic acid (TA1), by releasing histamine, induces itch and increases noxious painful heat stimuli.Itch was evaluated after s.c. administration of TA1 (0.4, 1.32 4 μg·kg(-1) ). Mice threshold (NHT) stimuli were the increasing-temperature hot plate (from 45.5 49.5°C) or (51.5°C) test, respectively, 15 min i.p. injection Itch, NHT pain evaluation repeated in mice pretreated pyrilamine....
Growing evidence indicates that transient receptor potential (TRP) channels contribute to different forms of pruritus. However, the endogenous mediators cause itch through signaling are poorly understood. In this study, we show genetic deletion or pharmacological antagonism TRPV4 attenuated in a mouse model psoriasis induced by topical application imiquimod. Human psoriatic lesions showed increased expression several microRNAs, including miR-203b-3p, which calcium ion response rodent dorsal...
: Transient receptor potential ankyrin 1 (TRPA1) activation is implicated in neuropathic pain-like symptoms. However, whether TRPA1 solely pain-signaling or contributes to neuroinflammation multiple sclerosis (MS) unknown. Here, we evaluated the role underlying symptoms using two different models of MS.
Over 60% of women with endometriosis experience abdominopelvic pain and broader manifestations, including chronic back pain, fibromyalgia, fatigue, vulvodynia, migraine. Although the imbalance proinflammatory mediators, complement component C5a, is associated endometriosis-related mechanisms causing widespread C5a role remain unclear. Female mice exhibit increased plasma levels pain. We hypothesize Schwann cells involvement in endometriotic Here, we show that silencing receptor (C5aR1)...
3-Iodothyroacetic acid (TA1) is among the thyroid hormone (T3) metabolites that can acutely modify behavior in mice. This study aimed to investigate whether TA1 also able reduce neuron hyper-excitability and protect from excitotoxic damage.CD1 male mice were treated intraperitoneally with saline solution or (4, 7, 11, 33 μg/kg) before receiving 90 mg/kg pentylenetrazole subcutaneously. The following parameters measured: latency first seizure onset, number of experiencing seizures,...