A5033959827- Respiratory Support and Mechanisms
- RNA modifications and cancer
- Pneumonia and Respiratory Infections
- Cancer-related molecular mechanisms research
- Barrier Structure and Function Studies
- Long-Term Effects of COVID-19
- Intensive Care Unit Cognitive Disorders
- Ferroptosis and cancer prognosis
- Epigenetics and DNA Methylation
- Nosocomial Infections in ICU
- COVID-19 Clinical Research Studies
- RNA Research and Splicing
- Antibiotics Pharmacokinetics and Efficacy
- Obstructive Sleep Apnea Research
- Macrophage Migration Inhibitory Factor
- Neuroscience of respiration and sleep
- Sepsis Diagnosis and Treatment
- Antibiotic Resistance in Bacteria
- Airway Management and Intubation Techniques
- Thermal Regulation in Medicine
- RNA Interference and Gene Delivery
- Pluripotent Stem Cells Research
- Neurological Disease Mechanisms and Treatments
- Autophagy in Disease and Therapy
- Folate and B Vitamins Research
Universitat de Barcelona
2018-2025
Consorci Institut D'Investigacions Biomediques August Pi I Sunyer
2019-2025
Tianshui Normal University
2025
Yangtze University
2024-2025
Zhongnan Hospital of Wuhan University
2020-2024
Wuhan University
2020-2024
Hospital Clínic de Barcelona
2018-2022
Prince Charles Hospital
2021
Centro de Investigación Biomédica en Red de Enfermedades Respiratorias
2019
Jilin University
2013-2018
Folate is a water-soluble B vitamin involved in the synthesis of purines and pyrimidines one essential vitamins for human growth reproduction. deficiency due to low dietary intake, poor absorption folate, alterations folate metabolism genetic defects or drug interactions significantly increases risk diseases such as neural tube defects, cardiovascular disease, cancer, cognitive dysfunction. Recent studies have shown that can cause hyperhomocysteinemia, which hypertension high homocysteine...
Claudin-6 (CLDN6), a member of claudin transmembrane protein family, has recently been reported to be undetectable or at low levels in human breast cancer cell lines and tissues plays role suppression migration invasion cells. In addition, it is that CLDN6 expression regulated by DNA methylation various cancers lines. However, unclear how regulates expression. Here we show the mechanism which line MCF-7.RT-PCR, Western blot immunofluorescent staining were utilized investigate MCF-7...
Claudin-6 (CLDN6), a member of CLDN family and key component tight junction, has been reported to function as tumor suppressor in breast cancer. However, whether CLDN6 plays any role cancer chemoresistance remains unclear. In this study, we investigated the acquisition cells. We manipulated expression MCF-7 MCF-7/MDR cells with lv-CLDN6 CLDN6-shRNA manipulation lead different susceptibilities several chemotherapeutic agents these The cytotoxicity adriamycin (ADM), 5-fluorouracil (5-FU),...
Abstract Obstructive sleep apnea (OSA) is a common disorder characterized by intermittent hypoxia (IH) and associated with the occurrence development of nonalcoholic fatty liver disease (NAFLD). However, specific mechanism which OSA induces NAFLD remains unclear. Therefore, effective interventions are lacking. This study aims to investigate role ferroptosis in OSA‐related using clinical data analyses, cell‐based molecular experiments, animal experiments. Indicators function, lipid...
Abstract Background Streptococcus pneumoniae , a primary cause of community-acquired pneumonia (CAP), is typically treated with β-lactams and macrolides or quinolones. Corticosteroids are now recommended as adjunctive therapy in severe CAP to improve outcomes. I n this prospective randomized animal study, we evaluated the bactericidal efficacy various antibiotic regimens combined corticosteroids using porcine pneumococcal model. Results In 30 White-Landrace female pigs, was induced by...
Introduction Distal flap necrosis is a common problem in transplantation. Bletilla striata polysaccharide (BSP) the main medicinal component of traditional Chinese medicine striata. The purpose this study was to investigate mechanism BSP promoting survival. Methods control group, low, medium and high dose groups, + autophagy inhibitor 3-methyladenine (3-MA) group were designed establish model cross-boundary rat back. After 7 days postoperative administration, samples taken. Results optimal...
Claudin 6 (CLDN6), a member of tight junction protein claudin (CLDN) family, inhibits proliferation and induces apoptosis MCF-7 breast cancer cells. However, these molecular mechanisms CLDN6-induced remain largely elusive. We previously found that restoration human CLDN6 gene expression was correlated with the level signal-regulating kinase 1 (ASK1) using cDNA array bioinformatics analysis. ASK1, mitogen-activated kinase, is involved in environmental stress-activation c-jun N-terminal (JNK)...
Abstract Background Tight junctions (TJs) are mainly composed of claudins, occludin, and tight junction adhesion molecules (JAM). The invasive metastatic phenotype highly cancer cells has been related to abnormal structure function TJs, with expression activated matrix metalloproteinases (MMPs). relevance these mechanisms responsible for the invasion metastasis ovarian carcinoma is unclear. Similarly, it not known if claudin-6, occludin MMP2 clinical properties tumors. Methods Expression was...
Cancers have a multifactorial etiology part of which is genetic. Recent data indicate that expression the tight junction claudin proteins involved in and progression cancer.To explore correlations claudin-2,-6, -11 pathogenesis clinical behavior gastric cancer, 40 cancer tissues 28 samples non-neoplastic adjacent to tumors were examined for by streptavidin-perosidase immunohistochemical staining method.The positive rates claudin-2 25% 68% respectively (P<0.001). The claudin-6 55% 79%...
Multidrug resistance (MDR) is a major problem in the treatment of breast cancer. In present study, next-generation sequencing technology was employed to identify differentially expressed genes MCF‑7/MDR cells and MCF‑7 cells, aimed investigate underlying molecular mechanisms MDR Differentially between were selected using software; total 2085 screened as cells. Furthermore, gene ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) pathway enrichment analyses performed DAVID database....
Pseudomonas aeruginosa pneumonia is commonly treated with systemic antibiotics to ensure adequate treatment of multidrug resistant (MDR) bacteria. However, intravenous (IV) often achieve suboptimal pulmonary concentrations. We therefore aimed evaluate the effect inhaled amikacin (AMK) plus IV meropenem (MEM) on bactericidal efficacy in a swine model monolateral MDR P. pneumonia.We ventilated 18 pigs for up 102 h. At 24 h after bacterial challenge, animals were randomized receive 72 either...
The downregulation of tight junction protein CLDN6 promotes breast cancer cell migration and invasion; however, the exact mechanism underlying remains unclear. silence is associated with DNA methyltransferase 1 (DNMT1) mediated methylation, DNMT1 regulated by transforming growth factor beta (TGFβ)/SMAD pathway. Therefore, we hypothesized that TGFβ/SMAD pathway, specifically SMAD2, may play a critical role for through methylation. To test this hypothesis, blocked SMAD2 pathway SB431542 in two...
A novel zeolitic imidazolate framework (ZIF)-based system for methylprednisolone (MP) delivery in the inner ear was developed and exhibited good protection of mice from noise low side effects.
Hypoxia is a common feature of laryngocarcinoma. Alterations in lipid metabolism are an important metabolic rewiring phenomenon for malignant cells to maintain their rapid proliferation the hypoxic microenvironment, which makes most cancers, including laryngocarcinoma, difficult cure. However, mechanisms involved laryngocarcinoma still unclear. This study aimed clarify changes under conditions and explore related mechanisms.Hep2 were incubated normoxic or environment (5% CO2 1% O2) at 37 °C...
Shortage of red blood cells (RBCs, erythrocytes) can have potentially life-threatening consequences for rare or unusual type patients with massive loss resulting from various conditions. Erythrocytes been derived human pluripotent stem (PSCs), but the risk potential tumorigenicity cannot be ignored, and a majority these produced PSCs express embryonic ε - fetal γ -globins little no adult β -globin remain nucleated. Here we report method to generate erythrocytes hair follicle mesenchymal...
Background N6-Methyladenosine (m 6 A), which is a prevalent regulator of mRNA expression, has gathered increasing study interests. Though the role m A as being important in many biological processes (such growth and proliferation cancers) been well documented, its potential tumor immune microenvironment (TIME) rarely analyzed. Methods We downloaded RNA single nucleotide polymorphism (SNP), copy number variation (CNV) data from The Cancer Genome Atlas (TCGA). then curated 21 regulators...
Obstructive sleep apnea (OSA), characterized by intermittent hypoxia (IH), may increase the risk of cancer development and a poor prognosis. TAMs M2 phenotype, together with hypoxic environment within tumor, drive tumor aggressiveness. However, mechanism in IH remains unclear. In our study, induced recruitment macrophages, IH-induced M2-like promoted glycolysis laryngeal cells through hexokinase 1. The inhibitor 2-deoxy-D-glucose HK1 shRNA were applied to verify this finding, confirming that...
Claudin-6 (CLDN6) is an integral component of the tight junction proteins in polarized epithelial and endothelial cells plays a crucial role maintaining cell integrity. Deregulation CLDN6 expression distribution tumor tissues have been widely documented correlated with cancer progression metastasis. However, complete mechanistic understanding regulation function remains to be studied. Herein, we show new potential properties functions from bioinformatics analysis. Using numerous algorithms...