A5101580909- Barrier Structure and Function Studies
- Chemotherapy-induced cardiotoxicity and mitigation
- Electron Spin Resonance Studies
- Cancer-related molecular mechanisms research
- Cardiac Ischemia and Reperfusion
- Epigenetics and DNA Methylation
- Ferroptosis and cancer prognosis
- Immune Response and Inflammation
- Traditional Chinese Medicine Analysis
- Atherosclerosis and Cardiovascular Diseases
- Takotsubo Cardiomyopathy and Associated Phenomena
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Antimicrobial Peptides and Activities
- Sirtuins and Resveratrol in Medicine
- Caveolin-1 and cellular processes
- Calcium signaling and nucleotide metabolism
- Peroxisome Proliferator-Activated Receptors
- Seaweed-derived Bioactive Compounds
- Advanced Welding Techniques Analysis
- Welding Techniques and Residual Stresses
- Circular RNAs in diseases
- Neurological Disease Mechanisms and Treatments
- Histone Deacetylase Inhibitors Research
- Cell death mechanisms and regulation
- Analytical Chemistry and Sensors
Beijing Tian Tan Hospital
2019-2024
Capital Medical University
2013-2024
Fujian Medical University
2024
CCCC Wuhan Harbour Engineering Design and Research (China)
2024
Beijing Anzhen Hospital
2013-2018
Jilin University
2011-2018
Jilin Medical University
2015
Beijing Hospital
2013
Chinese Academy of Medical Sciences & Peking Union Medical College
2013
Background: SIRT1, which belongs to the Sirtuin family of NAD-dependent enzymes, plays diverse roles in aging, metabolism, and disease biology. It could regulate cell survival has been shown be a protective factor heart function. Hence, we verified mechanism by SIRT1 regulates doxorubicin induced cardiomyocyte injury vivo vitro. Methods: We analyzed expression doxorubicin-induced neonatal rat model adult mouse failure model. was over-expressed cultured adenovirus mediated gene transfer....
Doxorubicin, a potent chemotherapeutic agent, is associated with acute and chronic cardiotoxicity, which cumulatively dose-dependent. Astragalus polysaccharide (APS), the extract of membranaceus strong antitumor antiglomerulonephritis activity, can effectively alleviate inflammation. However, whether APS could ameliorate chemotherapy-induced cardiotoxicity not understood. Here, we investigated protective effects on doxorubicin-induced elucidated underlying mechanisms APS. We analyzed...
// Yuan Cao 1 , Tao Shen Xiuqing Huang Yajun Lin Beidong Chen Jing Pang Guoping Li Que Wang Sylvia Zohrabian 2 Chao Duan 3 Yang Ruan 4 Yong Man Shu Jian Peking University Fifth School of Clinical Medicine, The MOH Key Laboratory Geriatrics, Beijing Hospital, National Center Gerontology, Beijing, 100730, China Department Cardiology, Boston Children's Enders 1250, Boston, MA, 02115, US Plant Resources Research and Development, Technology Business University, 100037, Anzhen Capital Medical...
Claudin-6 (CLDN6), a member of claudin transmembrane protein family, has recently been reported to be undetectable or at low levels in human breast cancer cell lines and tissues plays role suppression migration invasion cells. In addition, it is that CLDN6 expression regulated by DNA methylation various cancers lines. However, unclear how regulates expression. Here we show the mechanism which line MCF-7.RT-PCR, Western blot immunofluorescent staining were utilized investigate MCF-7...
Claudin-6 (CLDN6), a member of CLDN family and key component tight junction, has been reported to function as tumor suppressor in breast cancer. However, whether CLDN6 plays any role cancer chemoresistance remains unclear. In this study, we investigated the acquisition cells. We manipulated expression MCF-7 MCF-7/MDR cells with lv-CLDN6 CLDN6-shRNA manipulation lead different susceptibilities several chemotherapeutic agents these The cytotoxicity adriamycin (ADM), 5-fluorouracil (5-FU),...
Tremella fuciformis polysaccharide (TFPS), which is the extract of Berk, has previously been demonstrated to exhibit potent anti‑oxidative, anti‑inflammatory and anti‑aging effects. However, mechanisms underlying these protective therapeutic effects remain be elucidated. The aim present study was investigate TFPS on hydrogen peroxide‑induced injury human skin fibroblasts elucidate aforementioned mechanisms. A fibroblast model firstly established. MTT reactive oxygen species (ROS) production...
Background . Sirtuin 1 (SIRT1) is a member of the sirtuin family, which could activate cell survival machinery and has been shown to be protective in regulation heart function. Here, we determined mechanism by SIRT1 regulates Angiotensin II- (AngII-) induced cardiac hypertrophy injury vivo vitro. Methods We analyzed expression hearts control AngII-induced mouse hypertrophy. Female C57BL/6 mice were ovariectomized pretreated with 17 β -estradiol measure expression. Protein synthesis,...
Background: The only human cathelicidin, LL-37, and the innate defense regulator peptide IDR-1, which have been proven to antimicrobial activity, represent essential elements of immunity. Our previous study showed that LL-37 was protective in vitro attenuate LTA-induced inflammatory effects. Methicillin-resistant staphylococcus aureus (MRSA) causes a multitude serious sometimes life-threatening diseases around globe. However, effect IDR-1 MRSA-induced pneumonia is unknown. In present study,...
The usage of doxorubicin is hampered by its life-threatening cardiotoxicity in clinical practice. Dexrazoxane the only cardioprotective medicine approved FDA for preventing doxorubicin-induced cardiac toxicity. Nevertheless, mechanism dexrazoxane incompletely understood. aim our study to investigate possible molecular against cardiotoxicity. We established a mouse and cardiomyocyte injury model. Male C57BL/6J mice were randomly distributed into control group (Con), treatment (DOX), plus...
Claudin 6 (CLDN6), a member of tight junction protein claudin (CLDN) family, inhibits proliferation and induces apoptosis MCF-7 breast cancer cells. However, these molecular mechanisms CLDN6-induced remain largely elusive. We previously found that restoration human CLDN6 gene expression was correlated with the level signal-regulating kinase 1 (ASK1) using cDNA array bioinformatics analysis. ASK1, mitogen-activated kinase, is involved in environmental stress-activation c-jun N-terminal (JNK)...
Multidrug resistance (MDR) is a major problem in the treatment of breast cancer. In present study, next-generation sequencing technology was employed to identify differentially expressed genes MCF‑7/MDR cells and MCF‑7 cells, aimed investigate underlying molecular mechanisms MDR Differentially between were selected using software; total 2085 screened as cells. Furthermore, gene ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) pathway enrichment analyses performed DAVID database....
The downregulation of tight junction protein CLDN6 promotes breast cancer cell migration and invasion; however, the exact mechanism underlying remains unclear. silence is associated with DNA methyltransferase 1 (DNMT1) mediated methylation, DNMT1 regulated by transforming growth factor beta (TGFβ)/SMAD pathway. Therefore, we hypothesized that TGFβ/SMAD pathway, specifically SMAD2, may play a critical role for through methylation. To test this hypothesis, blocked SMAD2 pathway SB431542 in two...
Aim. To investigate the function of Tremella fuciformis polysaccharides (TFPS) in LPS-induced inflammation and oxidative stress macrophages. Methods . RAW264.7 cells were pretreated with TFPS then stimulated 0.1 μ g/ml LPS. NF κ B, Akt, p38MAPK, MCP-1, SOD-1 analyzed by Western blotting. Cell viability was measured using MTT assays. Reactive oxygen species (ROS) production, real-time PCR, ELISA, immunofluorescence staining performed on that treated LPS and/or to anti-inflammatory effect...
Long noncoding RNAs (lncRNAs) have been considered as crucial regulators of acute myocardial infarction (AMI). In this study, to analyze the effect differentiation antagonizing nonprotein coding RNA (DANCR) lncRNA on cardiomyocyte damage in AMI, injury was induced by oxygen-glucose deprivation (OGD). Cell counting kit-8 (CCK-8) assay and flow cytometry were used assess cell viability apoptosis, respectively. Quantitative real-time PCR measure expression levels DANCR miR-19a-3p....
Claudin-6 (CLDN6) is an integral component of the tight junction proteins in polarized epithelial and endothelial cells plays a crucial role maintaining cell integrity. Deregulation CLDN6 expression distribution tumor tissues have been widely documented correlated with cancer progression metastasis. However, complete mechanistic understanding regulation function remains to be studied. Herein, we show new potential properties functions from bioinformatics analysis. Using numerous algorithms...