A5087997862- Transplantation: Methods and Outcomes
- Organ Transplantation Techniques and Outcomes
- Mesenchymal stem cell research
- Mycobacterium research and diagnosis
- Respiratory viral infections research
- Renal Transplantation Outcomes and Treatments
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Tracheal and airway disorders
- Tissue Engineering and Regenerative Medicine
- Neonatal Respiratory Health Research
- Pluripotent Stem Cells Research
- Cancer-related molecular mechanisms research
- Cystic Fibrosis Research Advances
- Polyomavirus and related diseases
- Tuberculosis Research and Epidemiology
- Congenital Heart Disease Studies
- Congenital heart defects research
- Respiratory Support and Mechanisms
- Bacteriophages and microbial interactions
- Pulmonary Hypertension Research and Treatments
- Actinomycetales infections and treatment
- Corneal Surgery and Treatments
- Electrospun Nanofibers in Biomedical Applications
- Mechanical Circulatory Support Devices
- CRISPR and Genetic Engineering
Great Ormond Street Hospital for Children NHS Foundation Trust
2013-2024
Great Ormond Street Hospital
2004-2023
University College London
2004-2023
The Queen's Medical Research Institute
2020
University of Edinburgh
2017-2020
University of Bristol
2010-2019
National Health Service
2019
NIHR Bristol Cardiovascular Biomedical Research Unit
2010-2019
University of Udine
2015
Evolutionary Genomics (United States)
2015
Abstract Background Nontuberculous Mycobacterium infections, particularly abscessus, are increasingly common among patients with cystic fibrosis and chronic bronchiectatic lung diseases. Treatment is challenging due to intrinsic antibiotic resistance. Bacteriophage therapy represents a potentially novel approach. Relatively few active lytic phages available there great variation in phage susceptibilities M. abscessus isolates, requiring personalized identification. Methods isolates from 200...
Abstract Epithelial-mesenchymal transition (EMT) occurs during embryonic development and may also be associated with the metastatic spread of epithelial tumors. During EMT, E-cadherin is down-regulated this correlates increased motility invasion cells. We show that differentiation human stem (ES) cells in monolayer culture an E- to N-cadherin switch, vimentin expression, up-regulation repressor molecules (Snail Slug proteins), gelatinase (matrix metalloproteinases; MMP-2 MMP-9) activity...
To develop an embryoid body-free directed differentiation protocol for the rapid generation of functional vascular endothelial cells derived from human embryonic stem (hESCs) and to assess system microRNA regulation angiogenesis.The production defined cell lineages hESCs is a critical requirement evaluating their potential in regenerative medicine. We developed feeder- serum-free protocol. Directed revealed loss pluripotency markers progressive induction mRNA protein expression (including...
Rationale: Optimization of cell therapy for cardiac repair may require the association different populations with complementary activities. Objective: Compare reparative potential saphenous vein–derived pericytes (SVPs) that stem cells (CSCs) in a model myocardial infarction, and investigate whether combined transplantation provides further improvements. Methods Results: SVPs CSCs were isolated from vein leftovers coronary artery bypass graft surgery discarded atrial specimens transplanted...
Epithelial-mesenchymal transition (EMT) events occur during embryonic development and are important for the metastatic spread of epithelial tumors. We show here that spontaneous differentiation mouse stem (ES) cells is associated with an E- to N-cadherin switch, up-regulation E-cadherin repressor molecules (Snail Slug proteins), gelatinase activity (matrix metalloproteinase [MMP]-2 -9), increased cellular motility, all characteristic EMT events. The 5T4 oncofetal antigen, previously shown be...
Section:ChooseTop of pageAbstract <<Lung Transplantation for ...Pediatric Lung Transplant...Operative and Perioperati...(Non)-Adherence in Cystic...The Importance Measuri...Three Sociohistorical Cha...Alternate Models Selec...Some Comments on Study De...Lung ...ReferencesCITING ARTICLES
Brittle cornea syndrome (BCS; MIM 229200) is an autosomal recessive generalized connective tissue disorder caused by mutations in ZNF469 and PRDM5. It characterized extreme thinning fragility of the that may rupture absence significant trauma leading to blindness. Keratoconus or keratoglobus, high myopia, blue sclerae, hyperelasticity skin without excessive fragility, hypermobility small joints are additional features BCS. Transcriptional regulation extracellular matrix components,...
Background Living grafts produced by combining autologous heart‐resident stem/progenitor cells and tissue engineering could provide a new therapeutic option for definitive correction of congenital heart disease. The aim the study was to investigate antigenic profile, expansion/differentiation capacity, paracrine activity, pro‐angiogenic potential cardiac pericytes assess their engrafting capacity in clinically certified prosthetic grafts. Methods Results CD 34 pos cells, negative endothelial...
Abstract Aims Long non-coding RNAs (lncRNAs) play functional roles in physiology and disease, yet understanding of their contribution to endothelial cell (EC) function is incomplete. We identified lncRNAs regulated during EC differentiation investigated the role LINC00961 its encoded micropeptide, small regulatory polypeptide amino acid response (SPAAR), function. Methods results Deep sequencing human embryonic stem ECs was combined with Encyclopedia DNA Elements (ENCODE) RNA-seq data from...
Background Transplantation of adventitial pericytes ( APC s) promotes cardiac repair in murine models myocardial infarction. The aim present study was to confirm the benefit therapy a large animal model. Methods and Results We performed blind, randomized, placebo‐controlled trial swine model reperfused A first used human s hAPC from patients undergoing coronary artery bypass graft surgery. second allogeneic sAPC s). Primary end points were (1) ejection fraction as assessed by magnetic...
Abstract Background Children with cystic fibrosis (CF) can develop life-threatening infections of Mycobacterium abscessus. These present a significant clinical challenge, particularly when the strains involved are resistant to antibiotics. Recent evidence within-patient subclones M. abscessus in adults CF suggests possibility that diversity may be relevant for treatment pediatric patients. Methods We performed whole-genome sequencing (WGS) on 32 isolates were taken from multiple body sites 2...
<b>Background:</b> Recent reports have shown evidence of host derived parenchymal engraftment in several human allografts including the lung, leading to speculation that stem cell therapy may be useful for lung repair diseases such as cystic fibrosis (CF). To date, previous studies looked at single surgical or autopsy specimens and no longitudinal been reported. The aim this study was assess whether transbronchial biopsies could used time course chimerism following transplantation....
Objective— We investigated the association between functional, epigenetic, and expressional profile of human adventitial progenitor cells (APCs) therapeutic activity in a model limb ischemia. Approach Results— Antigenic functional features were analyzed throughout passaging 15 saphenous vein (SV)–derived APC lines, which 10 from SV leftovers coronary artery bypass graft surgery 5 varicose removal. Moreover, SV-APC lines transplanted (8×10 cells, IM) mice with Blood flow capillary arteriole...
Cell therapy represents a promising option for revascularization of ischemic tissues.However, injection dispersed cells is not optimal to ensure precise homing into the recipient's vasculature.Implantation cell-engineered scaffolds around occluded artery may obviate these limitations.Here, we employed synthetic polymer polycaprolactone (PCL) fabrication 3D woodpile-or channel-shaped by computer-assisted writing system (Pressure Assisted Micro-syringe Square (PAM 2 )), followed deposition...
The E-protein transcription factors play essential roles in lymphopoiesis, with E12 and E47 (hereafter called E2A) being particularly important B cell specification maturation. E2A gene is also involved a chromosomal translocation that results the leukemogenic oncoprotein E2A-PBX1. two activation domains of E2A, AD1 AD2, display redundant, independent, cooperative functions cell-dependent manner. by binding transcriptional co-activator CBP/p300; this interaction required oncogenesis occurs...
Abstract Background Mycobacterium abscessus infection has been associated with variable outcomes following lung transplantation. M comprises three subspecies ( subsp , massiliense and bolletii ). We investigated whether transplantation outcome in cystic fibrosis (CF) patients a single center was related to the genetic cluster. Methods CF chronic transplanted at Great Ormond Street Hospital between 2004 2017 were retrospectively examined. All isolates identified level by polymerase chain...