A5070364192- Adipose Tissue and Metabolism
- Tendon Structure and Treatment
- Vestibular and auditory disorders
- Sports injuries and prevention
- Bacterial biofilms and quorum sensing
- Muscle metabolism and nutrition
- Mitochondrial Function and Pathology
- Hearing, Cochlea, Tinnitus, Genetics
- Muscle Physiology and Disorders
- Advanced Electron Microscopy Techniques and Applications
- Shoulder Injury and Treatment
- Birth, Development, and Health
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Ear and Head Tumors
- Orthopedic Surgery and Rehabilitation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cardiovascular Effects of Exercise
- Tattoo and Body Piercing Complications
- Exercise and Physiological Responses
- Legionella and Acanthamoeba research
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Lipid metabolism and biosynthesis
- Connexins and lens biology
- Glycogen Storage Diseases and Myoclonus
University of Copenhagen
2015-2024
Rigshospitalet
1993-2024
Copenhagen University Hospital
2004-2024
Technical University of Denmark
2023
Clinical Research Management
2016
Regenerative Medicine Institute
2016
Capital Region of Denmark
2012
Bispebjerg Hospital
2009
University of Southern Denmark
2009
Czech Academy of Sciences, Institute of Microbiology
2007
The study investigated the distribution of silver after 28 days repeated oral administration nanoparticles (AgNPs) and acetate (AgAc) to rats. Oral is a relevant route exposure because use in products related food contact materials.AgNPs were synthesized with size 14 ± 4 nm diameter (90% nanoparticle volume) stabilized aqueous suspension by polymer polyvinylpyrrolidone (PVP). AgNPs remained stable throughout duration 28-day toxicity organ pattern following AgAc was similar. However absolute...
Disruption to proteins within the myofibre after a single bout of unaccustomed eccentric exercise is hypothesized induce delayed onset muscle soreness and be associated with an activation satellite cells. This has been shown in animal models using electrical stimulation but not humans voluntary exercise. Untrained males (n=8, range 22-27 years) performed 210 maximal contractions each leg on isokinetic dynamometer, voluntarily (VOL) one electrically induced (ES) other leg. Assessments from...
For a chronic infection to be established, bacteria must able cope with hostile conditions such as low iron levels, oxidative stress, and clearance by the host defense, well antibiotic treatment. It is generally accepted that biofilm formation facilitates tolerance these adverse conditions. However, microscopic investigations of samples isolated from sites infections seem suggest some do not need attached surfaces in order establish infections. In this study we employed scanning electron...
Significance Cancer cells characteristically express proteins with immature O-glycosylation, but how and why cancer O-glycans has remained poorly understood. Here, we report that one prevalent mechanism in pancreatic is epigenetic silencing, rather than somatic mutations a key chaperone, core 1 β3-Gal-T-specific molecular chaperone ( COSMC ), required for mature elongated O-glycosylation. We also demonstrate, the use of well-defined cell systems generated by precise gene editing, aberrant...
Patellar tendinopathy is characterized by pathologic abnormalities. Heavy slow resistance training (HSR) effective in the management of patellar tendinopathy, but underlying functional mechanisms remain elusive.To investigate fibril morphology and mechanical properties effect HSR on these properties.Cohort study; Level evidence, 2.Eight male patients with completed 12 weeks HSR. Nine healthy subjects served as controls. Assessments were conducted at baseline weeks. Patients assessed...
The purpose of this study was to test the hypothesis that remodeling skeletal muscle extracellular matrix (ECM) is involved in protecting human against injury. Biopsies were obtained from medial gastrocnemius muscles after a single bout electrical stimulation (B) or repeated (RB) 30 d later, (RBc). A biopsy collected control leg for comparison with stimulated leg. Satellite cell content, tenascin C, and regeneration assessed by immunohistochemistry; real-time PCR used measure mRNA levels...
Activation of energy expenditure in thermogenic fat is a promising strategy to improve metabolic health, yet the dynamic processes that evoke this response are poorly understood. Here we show synthesis mitochondrial phospholipid cardiolipin indispensable for stimulating and sustaining function. Cardiolipin biosynthesis robustly induced brown beige adipose upon cold exposure. Mimicking through overexpression synthase (Crls1) enhances consumption mouse human adipocytes. Crls1 deficiency...
The knowledge about the effect of estradiol on tendon connective tissue is limited. Therefore, we studied influence synthesis, structure, and biomechanical properties in postmenopausal women. Nonusers (control, n = 10) or habitual users oral replacement therapy (ERT, were at rest response to one-legged resistance exercise. Synthesis collagen was determined by stable isotope incorporation [fractional synthesis rate (FSR)] microdialysis technique (NH 2 -terminal propeptide type I synthesis)....
Adipose tissue exerts important endocrine and metabolic functions in health disease. Yet the bioenergetics of this is not characterized humans possible regional differences are elucidated. Using high resolution respirometry, mitochondrial respiration was quantified human abdominal subcutaneous intra-abdominal visceral (omentum majus) adipose from biopsies obtained 20 obese patients undergoing bariatric surgery. Mitochondrial DNA (mtDNA) genomic (gDNA) were determined by PCR technique for...
Bacterial biofilms are imaged by various kinds of microscopy including confocal laser scanning (CLSM) and electron (SEM). One limitation CLSM is its restricted magnification, which resolved the use SEM that provides high-magnification spatial images how single bacteria located interact within biofilm. However, conventional limited requirement dehydration samples during preparation. As consist mainly water, specimen might alter morphology. High magnification yet authentic important to...
Apolipoprotein M (ApoM) transports sphingosine-1-phosphate (S1P) in plasma, and ApoM-deficient mice (Apom–/–) have ~50% reduced plasma S1P levels. There are 5 known receptors, induces adherens junction formation between endothelial cells through the S1P1 receptor, which turn suppresses vascular leak. Increased permeability is a hallmark of inflammation. The purpose this study was to explore relationships leakage ApoM deficiency function normal physiology Vascular lungs assessed by...
Mitochondria undergo continuous changes in shape as result of complex fusion and fission processes. The physiological relevance mitochondrial dynamics is still unclear. In the field mitochondria bioenergetics, there a need tools to assess cell content. To develop method visualize networks high resolution volume.Confocal fluorescence microscopy imaging network stains human vastus lateralis single muscle fibres focused ion beam/ scanning electron (FIB/SEM) imaging, combined with 3D...
Mitochondrial potassium channels have been implicated in myocardial protection mediated through pre-/postconditioning. Compounds that open the Ca2+- and voltage-activated channel of big-conductance (BK) a pre-conditioning-like effect on survival cardiomyocytes after ischemia/reperfusion injury. Recently, mitochondrial BK (mitoBKs) were as infarct-limiting factors derive directly from KCNMA1 gene encoding for canonical BKs usually present at plasma membrane cells. However, some studies...
Tendons transmit muscle-generated force through an extracellular matrix of aligned collagen fibrils. The applied by the muscle at one end a microscopic fibril has to be transmitted macroscopic length tendon mechanisms that are poorly understood. A key element in this structure-function relationship is length. During embryogenesis short fibrils produced but they grow rapidly with maturation. There some controversy regarding adult tendon, mechanical data generally supporting discontinuity...
Cerebral malaria (CM) is caused by the binding of Plasmodium falciparum–infected erythrocytes (IEs) to brain microvasculature, leading inflammation, vessel occlusion, and cerebral swelling. We have previously linked dual intercellular adhesion molecule-1 (ICAM-1)– endothelial protein C receptor (EPCR)–binding P. falciparum parasites these symptoms, but mechanism driving pathogenesis has not been identified. Here, we used a 3D spheroid model blood–brain barrier (BBB) determine unexpected new...
Tendon fibroblasts synthesize collagen and form fibrils during embryonic development, but to what extent mature are able recapitulate development develop normal tendon structure is unknown. The present study examined the capability of human initiate fibrillogenesis when cultured in fixed-length fibrin gels. Fibroblasts were dissected from semitendinosus gracilis tendons healthy humans 3D linear synthesized an extracellular matrix parallel that aligned along axis tension. had a homogeneous...
Achromobacter xylosoxidans is an environmental opportunistic pathogen, which infects increasing number of immunocompromised patients. In this study we combined genomic analysis a clinical isolated A. strain with phenotypic investigations its important pathogenic features. We present complete assembly the genome NH44784-1996, isolate from cystic fibrosis patient obtained in 1996. The NH44784-1996 contains approximately 7 million base pairs 6390 potential protein-coding sequences. identified...
Moderate acoustic overexposure in adult rodents is known to cause acute loss of synapses on sensory inner hair cells (IHCs) and delayed degeneration the auditory nerve, despite completely reversible temporary threshold shift (TTS) morphologically intact cells. Our objective was determine whether a cochlear synaptopathy followed by neuropathy occurs after noise exposure pubescence, define neuropathic versus non-neuropathic levels for pubescent mice. While exposing 6 week old CBA/CaJ mice 8-16...
Two lipid membrane sculpting BAR domain proteins, PICK1 and ICA69, play a key role early in the biogenesis of peptide hormone secretory vesicles are critical for normal growth metabolic homeostasis.
Lipid metabolism is important for health and insulin action, yet the fundamental process of regulating lipid during muscle contraction incompletely understood. Here, we show that liver kinase B1 (LKB1) muscle-specific knockout (LKB1 MKO) mice display decreased fatty acid (FA) oxidation treadmill exercise. LKB1 MKO also SIK3 activity, increased histone deacetylase 4 expression, NAD+ concentration SIRT1 expression genes involved in FA oxidation. In AMP-activated protein (AMPK)α2 KO mice,...
Large mammals are capable of thermoregulation shortly after birth due to the presence brown adipose tissue (BAT). The majority BAT disappears and is replaced by white (WAT). We analyzed postnatal transformation in sheep with a time course study perirenal depot. observed changes morphology, gene expression metabolism within first two weeks life consistent expected transition from WAT. was characterized massively decreased mitochondrial abundance down-regulation related function oxidative...