A5037180539- Mitochondrial Function and Pathology
- ATP Synthase and ATPases Research
- Adipose Tissue and Metabolism
- Microbial Metabolic Engineering and Bioproduction
- Fungal and yeast genetics research
- Photosynthetic Processes and Mechanisms
- Cancer, Hypoxia, and Metabolism
- EEG and Brain-Computer Interfaces
- Soft Robotics and Applications
- Electrochemical sensors and biosensors
- Redox biology and oxidative stress
- Cell death mechanisms and regulation
- Metabolomics and Mass Spectrometry Studies
- Metabolism and Genetic Disorders
- NF-κB Signaling Pathways
- Biochemical effects in animals
- Electrochemical Analysis and Applications
- Photoreceptor and optogenetics research
- Cellular Mechanics and Interactions
- Advanced Glycation End Products research
- Immune Response and Inflammation
- Vitamin C and Antioxidants Research
- Neuroscience and Neuropharmacology Research
- Analytical Chemistry and Sensors
- Force Microscopy Techniques and Applications
Institut de Biochimie et Génétique Cellulaires
2014-2024
Centre National de la Recherche Scientifique
2015-2024
Université de Bordeaux
2014-2024
Bordeaux Population Health
2019
Institute of Continuous Media Mechanics
2018
Centre Hospitalier de l’Université de Montréal
2018
National Institutes of Health
1999-2003
Laboratory of Fundamental and Applied Bioenergetics
2001
Université Joseph Fourier
2001
National Cancer Institute
1999-2001
Although the molecular mechanisms of TNF signaling have been largely elucidated, principle that regulates balance life and death is still unknown. We report here domain kinase RIP, a key component complex, was cleaved by Caspase-8 in TNF-induced apoptosis. The cleavage site mapped to aspartic acid at position 324 RIP. demonstrated RIP resulted blockage NF-kappaB activation. RIPc, one products, enhanced interaction between TRADD FADD/MORT1 increased cells' sensitivity TNF. Most importantly,...
The death domain kinase, receptor interacting protein (RIP), is one of the major components tumor necrosis factor 1 (TNFR1) complex and plays an essential role in (TNF)-mediated nuclear κB (NF-κB) activation. activation NF-κB protects cells against TNF-induced apoptosis. Heat-shock proteins (Hsps) are chaperone molecules that confer stability help to restore native folding following heat shock other stresses. most abundant Hsp, Hsp90, also involved regulating function a number cell-signaling...
Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) (Apo2 [Apo2L]) is a member of the TNF superfamily and has been shown to have selective antitumor activity. Although it known that TRAIL (Apo2L) induces apoptosis activates NF-κB Jun N-terminal kinase (JNK) through receptors such as TRAIL-R1 (DR4) TRAIL-R2 (DR5), components its signaling cascade not well defined. In this report, we demonstrated death domain RIP essential for TRAIL-induced IκB (IKK) JNK activation. We found...
In numerous cell types, tumoral cells, proliferating bacteria, and yeast, respiration is inhibited when high concentrations of glucose are added to the culture medium. This phenomenon has been named "Crabtree effect." We used yeast investigate (i) short term event(s) associated with Crabtree effect (ii) a putative role hexose phosphates in inhibition respiration. Indeed, divide into "Crabtree-positive," where occurs, "Crabtree-negative," it does not. mitochondria isolated from these two...
The activation of IκB kinase (IKK) is a key step in the nuclear translocation transcription factor NF-κB. IKK complex composed three subunits: IKKα, IKKβ, and IKKγ (also called NEMO). In response to proinflammatory cytokine tumor necrosis (TNF), activated after being recruited TNF receptor 1 (TNF-R1) via receptor-associated 2 (TRAF2). We found that IKKα IKKβ catalytic subunits are required for IKK-TRAF2 interaction. This interaction occurs through leucine zipper motif common RING finger...
Summary Alzheimer's disease is the most common neurodegenerative disease, associated with aggregation of amyloid-β (Aβ) peptides. The exact mechanism neuronal cell dysfunction in poorly understood and numerous models have been used to decipher mechanisms leading cellular death. Yeast cells might be a good model understand intracellular toxicity triggered by Aβ Indeed, yeast has as examine protein functions or pathways that mediate secretion, subsequent proteins human disorders. In present...
Advanced glycation end-products (AGEs) trigger multiple metabolic disorders in the vessel wall that may turn lead to endothelial dysfunction. The molecular mechanisms by which AGEs generate these effects are not completely understood. Oxidative stress plays a key role development of deleterious occur endothelium during diabetes. Our main objectives were further understand how contribute reactive oxygen species (ROS) overproduction cells and evaluate protective effect an antioxidant plant...
We investigated the effects of 2,6‐diisopropylphenol on oxidative phosphorylation isolated rat liver mitochondria. Diisopropylphenol strongly inhibits state‐3 and uncoupled respiratory rates, when glutamate malate are substrates, as a direct consequence limitation electron transfer at level complex I. In addition, diisopropylphenol acts an uncoupler in non‐phosphorylating mitochondria, which leads to increase rate large decrease proton‐motive force. However, such cannot be due classical...
Mitochondrial biogenesis is a complex process. It necessitates the participation of both nuclear and mitochondrial genomes. This process highly regulated, content within cell varies according to energy demand. In yeast Saccharomyces cerevisiae, cAMP pathway involved in regulation biogenesis. An overactivation this leads an increase enzymatic content. Of three protein kinases, we have previously shown that Tpk3p one paper, investigated molecular mechanisms govern We show absence Tpk3p,...
Mitochondrial biogenesis is a complex process. It necessitates the contribution of both nuclear and mitochondrial genomes therefore crosstalk between nucleus mitochondria. now well established that cellular content can vary according to number stimuli physiological states in eukaryotes. The knowledge actors signals regulating thus high importance. redox state has been considered for long time as key element regulation various processes. In this paper, we report involvement oxidative stress...
// Emilie Charles 1, 6, * , Mehdi Hammadi Philippe Kischel 2 Vanessa Delcroix 6 Nicolas Demaurex 3 Cyril Castelbou Anne-Marie Vacher Anne Devin 4, Thomas Ducret 5, Paula Nunes Pierre 1 INSERM U1218, Institut Bergonié, Bordeaux, France Laboratory of Cellular and Molecular Physiology EA4667, Université de Picardie Jules Verne, SFR CAP-SANTE (FED 4231), Amiens, Department Cell Metabolism, University Geneva, Switzerland 4 Biochimie et Génétique Cellulaires, UMR 5095, 5 U1045, Centre Recherche...