Abstract Coronavirus disease 2019 (COVID-19) pandemic is affecting millions of patients worldwide. The consequences initial exposure to SARS-CoV-2 go beyond pulmonary damage, with a particular impact on lipid metabolism. Decreased levels in HDL-C were reported COVID-19 patients. Since HDL particles display antioxidant, anti-inflammatory and potential anti-infectious properties, we aimed at characterizing proteome functionality during relative healthy subjects. HDLs isolated from plasma 8...

Metabolic disorders related to obesity and type 2 diabetes are associated with aggravated cerebrovascular damages during stroke. In particular, hyperglycemia alters redox inflammatory status, leading cerebral endothelial cell dysfunction, blood-brain barrier (BBB) disruption brain homeostasis loss. Polyphenols constitute the most abundant dietary antioxidants exert anti-inflammatory effects that may improve complications in This study evaluated of characterized polyphenol-rich extract...

Extracellular vesicles (EVs), produced during viral infections, are of emerging interest in understanding infectious processes and host–pathogen interactions. EVs exosomes particular have the natural ability to transport nucleic acids, proteins, other components cellular or origin. Thus, they participate intercellular communication, immune responses, pathophysiological processes. Some viruses known hijack cell production content for their benefit. Here, we investigate whether two pathogenic...

Antirhea borbonica (A. borbonica) is an endemic plant from the Mascarene archipelago in Indian Ocean commonly used traditional medicine for its health benefits. This study aims (1) at exploring polyphenols profiles two types of extracts—aqueous (herbal infusion) and acetonic (polyphenol rich) extracts A. leaves—and (2) evaluating their potential toxicity vivo first time. We demonstrated that, whatever type extraction used, both displayed significant antioxidant properties acid phenolic...

The renal fibrotic process is characterized by a chronic inflammatory state and oxidative stress. Antirhea borbonica (A. borbonica) French medicinal plant found in Reunion Island known for its antioxidant anti-inflammatory activities mostly related to high polyphenols content. We investigated whether oral administration of polyphenol-rich extract from A. could exert vivo curative anti-renal fibrosis effect. To this aim, three days after unilateral ureteral obstruction (UUO), mice were daily...

Advanced glycation end-products (AGEs) trigger multiple metabolic disorders in the vessel wall that may turn lead to endothelial dysfunction. The molecular mechanisms by which AGEs generate these effects are not completely understood. Oxidative stress plays a key role development of deleterious occur endothelium during diabetes. Our main objectives were further understand how contribute reactive oxygen species (ROS) overproduction cells and evaluate protective effect an antioxidant plant...

Diabetes constitutes a major health problem associated with severe complications. In hyperglycemic conditions, chronically increased oxidation and glycation of circulating components lead to advanced end-products (AGEs) formation, key contributor in diabetes complication progression. line literature documenting the beneficial properties herbal teas, this study evaluates antioxidant/glycant Antirhea borbonica (Ab). Ab aqueous extract effects were tested on human albumin or erythrocytes...

High-density lipoproteins (HDLs) have multiple endothelioprotective properties. During SARS-CoV-2 infection, HDL-cholesterol (HDL-C) concentration is markedly reduced, and studies described severe impairment of the functionality HDL particles. Here, we report a multi-omic investigation first administration recombinant (rHDL) particles in COVID-19 patient an intensive care unit. Plasma ApoA1 increased HDL-C decreased after each injection, suggesting that these were functional terms reverse...

Abstract Overweight and obesity are worldwide health concerns leading to many physiological disorders. Recent data highlighted their deleterious effects on brain homeostasis plasticity, but the mechanisms underlying such disruptions still not well understood. In this study, we developed characterized a fast reliable diet-induced overweight (DIO) model in zebrafish, for (1) studying of overfeeding (2) testing different preventive and/or therapeutic strategies. By zebrafish 4 weeks, report...

Hyperglycemia alters the function of cerebral endothelial cells from blood-brain barrier, increasing risk cerebrovascular complications during diabetes. This study evaluated protective effect polyphenols on inflammatory and permeability markers bEnd3 exposed to high glucose concentration. Results show that hyperglycemic condition increased nuclear factor kappa B (NFκB) activity, deregulated expression interleukin-1 beta (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α),...

SARS-CoV-2 infection goes beyond acute pneumonia, as it also impacts lipid metabolism. Decreased HDL-C and LDL-C levels have been reported in patients with COVID-19. The profile is a less robust biochemical marker than apolipoproteins, components of lipoproteins. However, the association apolipoprotein during COVID-19 not well described understood. objective our study to measure plasma 14 apolipoproteins evaluate relationships between levels, severity factors patient outcomes. From November...

SARS-CoV-2 infection affects both lipid metabolism and lung function. The severity of the disease has been associated with reduced levels high-density lipoprotein (HDL) low-density cholesterol. Despite crucial role that these nanoparticles play in infection, few studies have examined their structure during COVID-19 beyond HDL quantity. study aimed to assess apolipoprotein patients who either survived or died following ICU admission. In addition, survivors non-survivors were compared for...

Hypericum lanceolatum Lam. (H. lanceolatum) is a traditional medicinal plant from Reunion Island used for its pleiotropic effects mainly related to antioxidant activity. The present work aimed 1) determine the potential toxicity of aqueous extract in vivo and 2) investigate putative biological properties using several zebrafish models oxidative stress, regeneration, estrogenicity, neurogenesis metabolic disorders. First, we characterized polyphenolic composition by liquid...

Scope Hyperglycemia alters cerebral endothelial cell and blood‐brain barrier functions, aggravating cerebrovascular complications such as stroke during diabetes. Redox inflammatory changes play a causal role. This study evaluates polyphenol protective effects in cells mouse model hyperglycemia. Methods results Murine bEnd.3 are exposed to characterized, polyphenol‐rich extract of Antirhea borbonica or its predominant constituent caffeic acid, Polyphenol on redox, vasoactive markers, infarct...

Obesity has reached epidemic proportions, and its prevalence tripled worldwide between 1975 2016, especially in Reunion Island, a French overseas region. Psiloxylon mauritianum, an endemic medicinal plant from Island registered the pharmacopeia, recently gained interest combating metabolic disorders because of traditional lipid-lowering "anti-diabetic" use. However, scientific data are lacking regarding toxicity real benefits on diseases. In this study, we aim to determine aqueous extract P....

Atherosclerosis and its associated clinical complications are major health issues in industrialized countries. Lipoprotein-associated phospholipase A2 (Lp-PLA2) was demonstrated to play an important role atherogenesis be a potential risk prediction factor of plaque rupture. Darapladib is one the most potent Lp-PLA2 inhibitors with IC50 0.25 nM. Using affinity for Lp-PLA2, we describe herein total synthesis darapladib radiolabeling precursor automated process positron emission tomography...

Methylglyoxal (MGO) is a highly reactive metabolite of glucose present at elevated levels in diabetic patients. Its cytotoxicity associated with endothelial dysfunction, which plays role cardiovascular and cerebrovascular complications. Although curcumin has many therapeutic benefits, these are limited due to its low bioavailability. We aimed improve the bioavailability evaluate potential synergistic effect reconstituted high-density lipoprotein (rHDL) nanoparticles (Cur-rHDLs) on...

: In Reunion, 27 medicinal plants have recently been registered in the French Pharmacopoeia. Among them, Ayapana triplinervis Vahl (A. triplinervis) is traditionally used to combat inflammation and oxidative stress, as well promote healing tissue regeneration. This work aims investigate toxicity of A. Aqueous Extract (AAE) using zebrafish model, determine its Maximum Non-Toxic Concentration (MNTC). Then, several pathological models established zebrafish, beneficial effects AAE at MNTC on...

Abstract PCSK9 best-known and studied function is to induce the hepatic degradation of low-density lipoprotein receptor (LDLR), thereby increasing concentration LDL-cholesterol (LDL-C) in blood. Beyond its effects on LDL, recent studies have reported pleiotropic notably septic shock, vascular inflammation, viral infection, cancer. While functional structural integrity peripheral nerves are critically influenced by circulating lipids, impact nervous system unknown. In this study, we...

<title>Abstract</title> <bold>Background: </bold>SARS-CoV-2 infection affects both lipid metabolism and lung function. The severity of the disease has been associated with reduced levels high-density lipoprotein (HDL) low-density cholesterol. Despite crucial role that these nanoparticles play in SARS-CoV-2 infection, few studies have examined their structure during COVID-19 beyond HDL quantity. study aimed to assess apolipoprotein patients who either survived or died following ICU admission....

Extracellular vesicles (EVs), produced during viral infections, are of emerging interest in understanding infectious processes and host-pathogen interactions. EVs exosomes particular have the natural ability to transport nucleic acids, proteins, other components cellular or origin. Thus, they participate intercellular communication, immune responses, pathophysiological processes. Some viruses known hijack cell production content for their benefit. Here, we investigated whether two pathogenic...

The endothelial integrity is the cornerstone of atherogenic process. Low-density lipoprotein (LDL) oxidation occurring within atheromatous plaques leads to deleterious vascular effects including cell cytotoxicity. aim this study was evaluate antioxidant and cytoprotective polyphenol-rich extracts from two medicinal plants Reunion Island: Antirhea borbonica (A. borbonica), Doratoxylon apetalum (D. apetalum). were obtained after dissolving each dry plant powder in an aqueous acetonic solution....