A5000971455- Lipoproteins and Cardiovascular Health
- Cancer, Lipids, and Metabolism
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Cholesterol and Lipid Metabolism
- HIV/AIDS Research and Interventions
- HIV, Drug Use, Sexual Risk
- Peroxisome Proliferator-Activated Receptors
- Atherosclerosis and Cardiovascular Diseases
- Sex work and related issues
- Drug Transport and Resistance Mechanisms
- Computational Drug Discovery Methods
- LGBTQ Health, Identity, and Policy
- Lipid metabolism and disorders
- Pharmaceutical Economics and Policy
- Cervical Cancer and HPV Research
- Plant biochemistry and biosynthesis
- Pancreatic function and diabetes
- Blood donation and transfusion practices
- Diabetes Treatment and Management
- Liver Disease Diagnosis and Treatment
- Systemic Lupus Erythematosus Research
- Various Chemistry Research Topics
- Reproductive tract infections research
- Acute Ischemic Stroke Management
- Diet, Metabolism, and Disease
Institut National de Santé Publique du Québec
2023-2025
Inserm
2016-2025
McGill University Health Centre
2023-2024
Écologie Marine Tropicale des Océans Pacifique et Indien
2016-2024
Centre Intégré Universitaire de Santé et de Services Sociaux du Centre-Sud-de-l'Île-de-Montréal
2024
University of Victoria
2024
Toronto Metropolitan University
2024
University of Windsor
2024
University of British Columbia
2024
University of Toronto
2023-2024
The numerous functions of the liver are controlled primarily at transcriptional level by concerted actions a limited number hepatocyte-enriched transcription factors (hepatocyte nuclear factor 1alpha [HNF1alpha], -1beta, -3alpha, -3beta, -3gamma, -4alpha, and -6 members c/ebp family). Of these, only HNF4alpha (nuclear receptor 2A1) HNF1alpha appear to be correlated with differentiated phenotype cultured hepatoma cells. HNF1alpha-null mice viable, indicating that this is not an absolute...
This 2022 European Atherosclerosis Society lipoprotein(a) [Lp(a)] consensus statement updates evidence for the role of Lp(a) in atherosclerotic cardiovascular disease (ASCVD) and aortic valve stenosis, provides clinical guidance testing treating elevated levels, considers its inclusion global risk estimation. Epidemiologic genetic studies involving hundreds thousands individuals strongly support a causal continuous association between concentration outcomes different ethnicities; is factor...
To elucidate the function of PPARγ in leptin-deficient mouse (ob/ob) liver, a liver-null on an ob/ob background, ob/ob-PPARγ(fl/fl)AlbCre+, was produced using floxed allele, PPARγ(fl/fl), and Cre recombinase under control albumin promoter (AlbCre). The liver ob/ob-PPARγ(fl/fl)AlbCre+ mice had deletion exon 2 corresponding loss full-length mRNA protein. PPARγ-deficient smaller dramatically decreased triglyceride (TG) content compared with equivalent lacking AlbCre transgene...
To elucidate the function of PPARγ in leptin-deficient mouse (ob/ob) liver, a liver-null on an ob/ob background, ob/ob-PPARγ(fl/fl)AlbCre+, was produced using floxed allele, PPARγ(fl/fl), and Cre recombinase under control albumin promoter (AlbCre). The liver ob/ob-PPARγ(fl/fl)AlbCre+ mice had deletion exon 2 corresponding loss full-length mRNA protein. PPARγ-deficient smaller dramatically decreased triglyceride (TG) content compared with equivalent lacking AlbCre transgene...
To address the importance of farnesoid X-receptor (FXR; NR1H4) for normal cholesterol homeostasis, we evaluated major pathways metabolism in the<i>FXR</i>-deficient (−/−) mouse model. Compared with wild-type,<i>FXR</i>(−/−) mice have increased plasma high density lipoprotein (HDL) and a markedly reduced rate HDL ester clearance. Concomitantly, <i>FXR</i>(−/−) exhibit expression hepatic genes involved reverse transport, most notably, that scavenger receptor BI.<i>FXR</i>(−/−) also increased:...
Disruption of the peroxisome proliferator-activated receptor γ (PPARγ) gene causes embryonic lethality due to placental dysfunction. To circumvent this, a PPARγ conditional knockout mouse was produced by using Cre-loxP system. The targeted allele, containing loxP sites flanking exon 2 gene, crossed into transgenic line expressing Cre recombinase under control alpha/beta interferon-inducible (MX) promoter. Induction MX promoter pIpC resulted in nearly complete deletion exon, corresponding...
The nuclear receptors, farnesoid X receptor (FXR) and pregnane (PXR), are important in maintaining bile acid homeostasis. Deletion of both FXR PXR vivo by cross-breeding B6;129-Fxrtm1Gonz (FXR-null) B6;129-Pxrtm1Glaxo-Wellcome (PXR-null) mice revealed a more severe disruption acid, cholesterol, lipid homeostasis Pxrtm1Glaxo-Wellcome (FXR-PXR double null or FPXR-null) fed 1% cholic (CA) diet. Hepatic expression the constitutive androstane (CAR) its target genes was induced FXR- FPXR-null CA...
Familial autosomal dominant hypercholesterolemia is associated with high risk for cardiovascular accidents and related to mutations in the low density lipoprotein receptor or its ligand apolipoprotein B (apoB). Mutations a third gene, proprotein convertase subtilisin kexin 9 (PCSK9), were recently this disease. PCSK9 acts as natural inhibitor of pathway, both genes are regulated by depletion cholesterol cell content statins, via sterol regulatory element-binding protein (SREBP). Here we...
Identification of mutations in the ABCA1 transporter (ABCA1) as genetic defect Tangier disease has generated interest modulating atherogenic risk by enhancing gene expression. To investigate role atherogenesis, we analyzed diet-induced atherosclerosis transgenic mice overexpressing human (hABCA1-Tg) and spontaneous lesion formation hABCA1-Tg x apoE-knockout (KO) mice. Overexpression hABCA1 C57BL/6 resulted a unique anti-atherogenic profile characterized decreased plasma cholesterol (63%),...
The discovery of the ABCA1 lipid transporter has generated interest in modulating human plasma HDL levels and atherogenic risk by enhancing gene expression. To determine if increased expression modulates metabolism vivo, we transgenic mice that overexpress (hABCA1-Tg). Hepatic macrophage hABCA1 enhanced cholesterol efflux to apoA-I; cholesterol, cholesteryl esters (CEs), free phospholipids, apoA-I apoB levels; led accumulation apoE-rich HDL1. transgene delayed 125I-apoA-I catabolism both...
The discovery of the ABCA1 lipid transporter has generated interest in modulating human plasma HDL levels and atherogenic risk by enhancing gene expression. To determine if increased expression modulates metabolism vivo, we transgenic mice that overexpress (hABCA1-Tg). Hepatic macrophage hABCA1 enhanced cholesterol efflux to apoA-I; cholesterol, cholesteryl esters (CEs), free phospholipids, apoA-I apoB levels; led accumulation apoE-rich HDL1. transgene delayed 125I-apoA-I catabolism both...
The farnesoid X receptor (FXR) is a bile acid-activated transcription factor that regulates the expression of genes critical for acid and lipid homeostasis. This study was undertaken to investigate pathological consequences loss FXR function on risk severity atherosclerosis. For this purpose, FXR-deficient (FXR−/−) mice were crossed with apolipoprotein E-deficient (ApoE−/−) generate FXR−/−ApoE−/− mice. Challenging these high-fat, high-cholesterol (HF/HC) diet resulted in reduced weight gain...
Proprotein convertase subtilisin/kexin type 9 (PCSK9) promotes the degradation of LDL receptor (LDLr) in hepatocytes, and its expression mouse liver has been shown to decrease with fenofibrate treatment.We developed a sandwich ELISA using recombinant human PCSK9 protein 2 affinity-purified polyclonal antibodies directed against PCSK9. We measured circulating concentrations 115 diabetic patients from FIELD (Fenofibrate Intervention Event Lowering Diabetes) study before after found that plasma...
Hepatocyte nuclear factor 4alpha (HNF4alpha; NR2A1) is an orphan member of the receptor superfamily expressed in liver and intestine. While HNF4alpha expression critical for function, its role gut pathogenesis inflammatory bowel disease (IBD) unknown.Human intestinal biopsies from control IBD patients were examined mRNAs encoding other receptors. An intestine-specific null mouse line (Hnf4alpha(DeltaIEpC)) was generated using Hnf4alpha-floxed allele villin-Cre transgene. These mice their...
Objective— The goal of this study was to investigate the effects nonenzymatic glycation on antiinflammatory properties apolipoprotein (apo) A-I. Methods and Results— Rabbits were infused with saline, lipid-free apoA-I from normal subjects (apoA-I N ), nonenzymatically glycated by incubation methylglyoxal Glyc in vitro diabetes vivo discoidal reconstituted high-density lipoproteins (rHDL) containing phosphatidylcholine , (A-I )rHDL, or )rHDL. At 24 hours postinfusion, acute vascular...
To elucidate how the proprotein convertase subtilisin kexin type 9 (PCSK9) inhibitor alirocumab modulates lipoprotein(a) [Lp(a)] plasma levels, authors performed a series of Lp(a) uptake studies in primary human hepatocytes and dermal fibroblasts measured secretion from hepatocytes. They found that cellular occurred low-density lipoprotein receptor–independent manner. Neither PCSK9 nor altered internalization. By contrast, apolipoprotein (a) was sharply increased by PCSK9, an effect reversed...
SummaryRabbits fed cholesterol-supplemented purified diets containing 20% of hydrogenated shortening or safflower oil showed lower plasma cholesterol levels on diets, but only negligible differences between oils with respect to aortic atheroma production. Similar studies cholesterol-free that rabbits coconut are much more prone hypercholesteremia than diets. In these latter atheromatous lesions developed in all 16 weeks saturated fat as contrasted lesion production oil.