A5034190142- Diverse Approaches in Healthcare and Education Studies
- Healthcare Education and Workforce Issues
- Health and Wellbeing Research
- Consumer Perception and Purchasing Behavior
- Education and Learning Interventions
- Diverse Topics in Contemporary Research
- Nutrition, Health and Food Behavior
- Psychosocial Factors Impacting Youth
- Educational Systems and Policies
- Cultural and Historical Studies
- Healthcare and Venom Research
- Food Quality and Safety Studies
- Education, Safety, and Science Studies
- Hydrogels: synthesis, properties, applications
- Hemoglobin structure and function
- Korean Urban and Social Studies
- Pharmacy and Medical Practices
- Nanoparticle-Based Drug Delivery
- Monoclonal and Polyclonal Antibodies Research
- Energy and Environmental Systems
- Agriculture, Soil, Plant Science
- Drug Transport and Resistance Mechanisms
- Technology and Data Analysis
- Radiopharmaceutical Chemistry and Applications
- Fashion and Cultural Textiles
Frederick National Laboratory for Cancer Research
2013-2025
Seoul National University of Science and Technology
2024
Changshin University
2021-2024
United States Food and Drug Administration
2013-2023
Center for Drug Evaluation and Research
2016-2023
Pusan National University Yangsan Hospital
2023
Catholic University of Korea
1998-2021
Yonsei University
2009-2021
Tongmyong University
2015-2021
Sungkyunkwan University
2004-2020
Bile acid concentrations are controlled by a feedback regulatory pathway whereby activation of the farnesoid X receptor (FXR) represses transcription both CYP7A1 gene, encoding rate-limiting enzyme in classic bile synthesis pathway, and CYP8B1 required for cholic acid. The tissue-specific roles FXR were examined using liver- intestine-specific FXR-null models. deficiency either liver (Fxr DeltaL) or intestine DeltaIE) increased pool size. Treatment with FXR-selective agonist GW4064...
The farnesoid X receptor (FXR) controls the synthesis and transport of bile acids (BAs). Mice lacking expression FXR, designated Fxr-null, have elevated levels serum hepatic BAs an increase in BA pool size. Surprisingly, at 12 months age, male female Fxr-null mice had a high incidence degenerative lesions, altered cell foci liver tumors including hepatocellular adenoma, carcinoma hepatocholangiocellular carcinoma, latter which is rarely observed mice. At 3 months, increased proinflammatory...
Most individuals with Down syndrome show early onset of Alzheimer disease (AD), resulting from the extra copy chromosome 21. Located on this is a gene that encodes dual specificity tyrosine phosphorylation-regulated kinase 1A (DYRK1A). One pathological hallmarks in AD presence neurofibrillary tangles (NFTs), which are insoluble deposits consist abnormally hyperphosphorylated Tau. Previously it was reported Tau at Thr-212 residue phosphorylated by Dyrk1A vitro. To determine physiological...
Pregnane X receptor (PXR) expression was shown to be protective in inflammatory bowel disease (IBD). However, the mechanism by which PXR provides protection remains unclear. Wild-type and Pxr-null mice were treated with agonist pregnenolone-16alpha-carbonitrile or vehicle administered 2.5% dextran sulfate sodium (DSS) drinking water induce IBD. Typical clinical symptoms evaluated on a daily basis. In vivo intestinal permeability assays proinflammatory cytokine analysis performed....
Hepatocyte nuclear factor 4alpha (HNF4alpha; NR2A1) is an orphan member of the receptor superfamily expressed in liver and intestine. While HNF4alpha expression critical for function, its role gut pathogenesis inflammatory bowel disease (IBD) unknown.Human intestinal biopsies from control IBD patients were examined mRNAs encoding other receptors. An intestine-specific null mouse line (Hnf4alpha(DeltaIEpC)) was generated using Hnf4alpha-floxed allele villin-Cre transgene. These mice their...
Hsp31 encoded by hchA is known as a heat-inducible molecular chaperone. Although structure studies revealed that has putative catalytic triad consisting of Asp-214, His-186 and Cys-185, its enzymatic function, besides weak amino-peptidase activity, still unknown. We found displays glyoxalase activity catalyses the conversion methylglyoxal (MG) to d-lactate without an additional cofactor. The was completely abolished in hchA-deficient strain, confirming relationship between gene vivo....
Photoimmunotherapy (PIT) is a new cancer treatment that combines the specificity of antibodies for targeting tumors with toxicity induced by photosensitizers after exposure to near infrared (NIR) light. Herein we compare two commonly available anti-EGFR monoclonal antibodies, cetuximab and panitumumab, their effectiveness as PIT agents in EGFR positive tumor models. A photosensitizer, IR-700, conjugated either (cet-IR700) or panitumumab (pan-IR700), was evaluated using EGFR-expressing A431...
Animal studies reveal that fasting and caloric restriction produce increased activity of specific metabolic pathways involved in resistance to weight loss liver. Evidence suggests this phenomenon may part occur through the action constitutive androstane receptor (CAR, NR1I3). Currently, precise molecular mechanisms activate CAR during are unknown. We show coordinately induces expression genes encoding peroxisome proliferator-activated gamma coactivator-1alpha (PGC-1alpha), CAR, cytochrome...
Summary Auranofin (AF) is a sulphur‐containing gold compound. Because of its anti‐inflammatory and immunosuppressive activities, AF has been widely used for the therapeutic treatment rheumatoid arthritis. However, little known about mechanism action. To elucidate molecular underlying effect AF, we studied effects on cellular responses to interleukin‐6 (IL‐6). In HepG2 human hepatoma cells, markedly inhibited IL‐6‐induced phosphorylation janus kinase 1 (JAK1) signal transducer activator...
Photoimmunotherapy is a highly cell-selective cancer therapy based on an armed antibody conjugate with phthalocyanine-based photosensitizer, IR700. induces rapid and specific necrosis in targeted cells after exposure to near-infrared (NIR) light. Cells not expressing the antigen are affected. To date, photoimmunotherapy has been demonstrated only full antibody-IR700 conjugates. In this study, small bivalent fragments, including anti–prostate-specific membrane (PSMA) diabody (Db) minibody...
Glypican-3 (GPC3) represents an attractive target for hepatocellular carcinoma (HCC) therapy because it is highly expressed in HCC but not adult normal tissue. Recently, high affinity anti-GPC3 antibodies have been developed; however, full may penetrate evenly into tumor parenchyma, reducing their effectiveness. In this study, we compared a whole IgG antibody, YP7, with human heavy chain HN3, regard to relative therapeutic effects. Both YP7 and HN3 bound GPC3-positive A431/G1 cells were...
The ease, noninvasiveness, and safety of oral fluid collection have increased the use this alternative matrix for drugs-of-abuse testing; however, few controlled drug administration data are available to aid in interpretation results.Single codeine doses (60 120 mg/70 kg) were administered 19 volunteers. Oral plasma analyzed free codeine, norcodeine, morphine, normorphine by solid-phase extraction combined with gas chromatography-mass spectrometry (SPE/GC-MS). Physiologic subjective effects...
Methylglyoxal (MG) is a toxic metabolite known to accumulate in various cell types. We detected vivo conversion of MG acetol MG-accumulating Escherichia coli cells by use (1)H nuclear magnetic resonance ((1)H-NMR) spectroscopy. A search for homologs the mammalian aldo-keto reductases (AKRs), which are exhibit activity MG, revealed nine open reading frames from E. genome. Based on both sequence similarities and preliminary characterization with (1)H-NMR crude extracts corresponding mutant...
Cholesterol homeostasis is maintained by coordinate regulation of cholesterol synthesis and its conversion to bile acids in the liver. The excretion from liver intestine regulated ATP-binding cassette half-transporters ABCG5 ABCG8. genes for these two proteins are closely linked divergently transcribed a common intergenic promoter region. Here, we identified binding site hepatocyte nuclear factor 4alpha (HNF4alpha) ABCG5/ABCG8 promoter, through which HNF4alpha strongly activated expression...
Human organic anion transporter 3 (hOAT3/SLC22A8) is predominantly expressed in the proximal tubules of kidney and plays a major role urinary excretion variety anions. The promoter region hOAT3 was characterized to elucidate mechanism underlying tissue-specific expression hOAT3. minimal identified be located approximately 300 base pairs upstream transcriptional start site, where there are canonical TATA hepatocyte nuclear factor (HNF1) binding motifs, which conserved rodent Oat3 genes....
Based on animal data, there is speculation that (±)-3,4-methylenedioxymethamphetamine (MDMA) neurotoxic to humans. Extrapolation of MDMA findings from animals humans requires assessment pharmacokinetics in various species, and low-dose administration data rats are lacking. In this study, we examine given low (2 mg/kg) high (10 doses racemic via intraperitoneal, subcutaneous, oral routes. Repeated blood specimens were collected venous catheters, plasma was assayed for its metabolites,...
Signal transducer and activator of transcription 3 (STAT3) telomerase are considered attractive targets for anticancer therapy. The in vitro activity the gold(I) compound auranofin was investigated using MDA-MB 231 human breast cancer cells, which STAT3 is constitutively active. In cell culture, inhibited growth a dose-dependent manner, N-acetyl-L-cysteine (NAC), scavenger reactive oxygen species (ROS), markedly blocked effect auranofin. Incorporation 5-bromo-2'-deoxyuridine into DNA...
Aim: Effectiveness of Glypican-3 (GPC3)-targeted photoimmunotherapy (PIT) combined with the nanoparticle albumin-bound paclitaxel (nab-paclitaxel) for hepatocellular carcinoma was evaluated. Materials & methods: GPC3 expressing A431/G1 cells were incubated a phthalocyanine-derivative, IRDye700DX (IR700), conjugated to an anti-GPC3 antibody, IR700-YP7 and exposed near-infrared light. Therapeutic experiments combining GPC3-targeted PIT nab-paclitaxel performed in tumor-bearing mice. Results:...
Plasmonic molecules are building blocks of metallic nanostructures that give rise to intriguing optical phenomena with similarities those seen in molecular systems. The ability design plasmonic hybrid structures and nanometric resolution would enable applications metamaterials sensing presently cannot be demonstrated, because a lack suitable fabrication methods allowing the structural control atoms on large scale. Here we demonstrate wafer-scale "lithography-free" parallel scheme realize...
Following the initial reports demonstrating feasibility of immunoPET imaging simian immunodeficiency virus (SIV) using gp120-targeting monoclonal antibodies in non-human primates, replication efforts system human (HIV)-infected individuals have yielded conflicting results. Herein, we used two anti-gp120 antibodies, 7D3 and ITS103.01LS-F(ab')2, to interrogate reproducibility probes for SIV rhesus macaques. The binding affinity estimates 89Zr radiolabeled ITS103.01LS-F(ab')2 gp120, in-vitro...
Valacyclovir is the 5′-valyl ester prodrug of acyclovir, an effective anti-herpetic drug. Systemic availability acyclovir in humans three to five times higher when administered orally as prodrug. The increased bioavailability valacyclovir attributed carrier-mediated intestinal absorption, via hPEPT1 peptide transporter, followed by rapid and complete conversion acyclovir. one or more human enzymes responsible for vivo activation active drug its sites, however, have not been identified. In...