A5034028155- Monoclonal and Polyclonal Antibodies Research
- Radiopharmaceutical Chemistry and Applications
- CAR-T cell therapy research
- Prostate Cancer Treatment and Research
- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Medical Imaging Techniques and Applications
- Glycosylation and Glycoproteins Research
- Medical Imaging and Pathology Studies
- Cancer Research and Treatments
- Advanced biosensing and bioanalysis techniques
- Lymphoma Diagnosis and Treatment
- Nanoplatforms for cancer theranostics
- Advanced Biosensing Techniques and Applications
- HER2/EGFR in Cancer Research
- Peptidase Inhibition and Analysis
- Protein purification and stability
- Cancer Cells and Metastasis
- Virus-based gene therapy research
- Mass Spectrometry Techniques and Applications
- Immune Cell Function and Interaction
- bioluminescence and chemiluminescence research
- Nanoparticle-Based Drug Delivery
- Cell Adhesion Molecules Research
- RNA and protein synthesis mechanisms
University of California, Irvine
2025
University of California, Los Angeles
2015-2024
Institute for Molecular Medicine
2014-2024
City Of Hope National Medical Center
2001-2024
City of Hope
2001-2024
Beckman Research Institute
2002-2024
ImaginAb (United States)
2016-2023
Molecular Biology Consortium
2004-2023
Indiana University – Purdue University Indianapolis
2021
University of California System
2013-2020
This study evaluates the influence of particle size, PEGylation, and surface coating on quantitative biodistribution near-infrared-emitting quantum dots (QDs) in mice. Polymer- or peptide-coated 64Cu-labeled QDs 2 12 nm diameter, with without polyethylene glycol (PEG) molecular weight 2000, are studied by serial micropositron emission tomography imaging region-of-interest analysis, as well transmission electron microscopy inductively coupled plasma mass spectrometry. PEGylation peptide slow...
Luciferases, which have seen expansive employment as reporter genes in biological research, could also be used applications where the protein itself is conjugated to ligands create probes that are appropriate for use small animal imaging. As bioluminescence activity of commonly luciferases too labile serum permit this application, specific mutations Renilla luciferase, selected using a consensus sequence driven strategy, were screened their ability confer stability well light output. Using...
While chemotherapy delivery by nanocarriers has modestly improved the survival prospects of pancreatic ductal adenocarcinoma (PDAC), additional engagement immune response could be game changing. We demonstrate a nano-enabled approach for accomplishing robust anti-PDAC immunity in syngeneic mice through induction immunogenic cell death (ICD) as well interfering immunosuppressive indoleamine 2,3-dioxygenase (IDO) pathway. This is accomplished conjugating IDO inhibitor, indoximod (IND), to...
Amplification of nucleic acids from paraffin-embedded material by the polymerase chain reaction (PCR) is increasingly being used to detect viral genomes and oncogene mutations. To determine effect fixation on preservation acids, we fixed two randomly chosen fresh pathology specimens in formalin, B-5, Bouin's, Zenker's, ethanol, Omnifix for 6, 24, 48, 72, 168 hr (1 week), then embedded tissue paraffin. Oligonucleotide primers specific cytoplasmic-beta-actin gene were span an intron such that...
Abstract The rapidly advancing field of cancer immunotherapy is currently limited by the scarcity noninvasive and quantitative technologies capable monitoring presence abundance CD8+ T cells other immune cell subsets. In this study, we describe generation 89Zr-desferrioxamine–labeled anti-CD8 cys-diabody (89Zr-malDFO-169 cDb) for immuno-PET tracking endogenous cells. We demonstrate that a sensitive tool detecting changes in systemic tumor-infiltrating CD8 expression preclinical syngeneic...
Immunotherapy is becoming the mainstay for treatment of a variety malignancies, but only subset patients responds to treatment. Tumor-infiltrating CD8-positive (CD8+) T lymphocytes play central role in antitumor immune responses. Noninvasive imaging CD8+ cells may provide new insights into mechanisms immunotherapy and potentially predict response. We are studying safety utility <sup>89</sup>Zr-IAB22M2C, radiolabeled minibody against cells, targeted with cancer. <b>Methods:</b> The initial...
An improved approach composed of an oxidation reaction in acidic H2O2 solution and a sequential silanization using neat silane reagents for surface modification poly(dimethylsiloxane) (PDMS) substrates was developed. This solution-phase is simple convenient some routine analytical applications chemistry biology laboratories designed intact PDMS-based microfluidic devices, with no device postassembly required. Using this approach, two different functional groups, poly(ethylene glycol) (PEG)...
Rapid imaging by antitumor antibodies has been limited the prolonged targeting kinetics and clearance of labeled whole antibodies. Genetically engineered fragments with rapid access high retention in tumor tissue combined blood are suitable for labeling short-lived radionuclides, including positron-emitting isotopes positron-emission tomography (PET). An fragment was developed from high-affinity anticarcinoembryonic antigen (CEA) monoclonal antibody T84.66. This single-chain variable (Fv)-C...
Abstract Antibody fragments are recognized as promising vehicles for delivery of imaging and therapeutic agents to tumor sites in vivo. The serum persistence IgG1 with intact Fc region is controlled by the protective neonatal receptor (FcRn) receptor. To modulate half-life engineered antibodies, we have mutated Fc-FcRn binding site chimeric anti–carcinoembryonic antigen (CEA) antibodies produced a single-chain Fv-Fc format. anti-CEA T84.66 format wild-type five mutants (I253A, H310A, H435Q,...
Identification of cancer cell–surface biomarkers and advances in antibody engineering have led to a sharp increase the development therapeutic antibodies. These same new generation radiolabeled antibodies fragments that can be used as cancer-specific imaging agents, allowing quantitative cell-surface protein expression vivo. Immuno–positron emission tomography (immunoPET) with intact has shown success clinically diagnosing staging cancer. Engineered fragments, such diabodies, minibodies,...
This study evaluates the quantitative biodistribution of commercially available CdSe quantum dots (QD) in mice. <b>Methods:</b><sup>64</sup>Cu-Labeled 800- or 525-nm emission wavelength QD (21- 12-nm diameter), with without 2,000 MW (molecular weight) polyethylene glycol (PEG), were injected intravenously into mice (5.55 MBq/25 pmol QD) and studied using well counting by serial microPET region-of-interest analysis. <b>Results:</b> Both methods show rapid uptake liver (27.4–38.9 %ID/g) (%ID/g...
The lineage and clonality of Hodgkin's disease (HD) were investigated by analyzing the organization immunoglobulin T-cell receptor beta-chain (T beta) gene loci in 18 cases HD, for comparison, a panel 103 B- non-Hodgkin's lymphomas (NHLs) lymphoid leukemias (LLs). Sizable clonal or populations, representing greater than equal to 10% pathologic sample, readily detectable immunogenotypic analysis all NHLs LLs but not any HD. However, extremely minor populations (less 1%) 3 We demonstrated that...
Significance Anti-CD8 immuno-PET imaging agents provide the potential to monitor localization, migration, and expansion of CD8-expressing cells noninvasively in vivo. Shown here is successful generation functional anti-CD8 based on engineered antibodies for use a variety preclinical disease immunotherapeutic models.