A5082377321- Monoclonal and Polyclonal Antibodies Research
- Radiopharmaceutical Chemistry and Applications
- Cancer Genomics and Diagnostics
- Molecular Biology Techniques and Applications
- Glycosylation and Glycoproteins Research
- Advanced Proteomics Techniques and Applications
- Genetic factors in colorectal cancer
- SARS-CoV-2 and COVID-19 Research
- Peptidase Inhibition and Analysis
- Clinical Laboratory Practices and Quality Control
- COVID-19 Clinical Research Studies
- Immune cells in cancer
- Immune Cell Function and Interaction
- Mass Spectrometry Techniques and Applications
- Genomics and Rare Diseases
- Immune Response and Inflammation
- Gene expression and cancer classification
- Retinal Diseases and Treatments
- Venous Thromboembolism Diagnosis and Management
- Retinal Imaging and Analysis
- SARS-CoV-2 detection and testing
- Protein purification and stability
- Cell Adhesion Molecules Research
- Atrial Fibrillation Management and Outcomes
- Metabolomics and Mass Spectrometry Studies
University Medical Centre Mannheim
2016-2025
Heidelberg University
2016-2025
University Hospital Heidelberg
2016-2025
Heidelberg University
1992-2025
Medizinische Fakultät Mannheim
2006-2024
Robert Koch Institute
2023
International Union of Pure and Applied Chemistry
2022
European Union of Medical Specialists
2022
University Hospital Leipzig
2022
United States Congress
2021
PURPOSE To establish a sensitive assay for the specific detection of carcinoembryonic antigen (CEA)-expressing tumor cells in bone marrow patients with colorectal cancer and other CEA-positive carcinomas. PATIENTS AND METHODS A CEA-specific nested reverse transcriptase (RT) polymerase chain reaction (PCR) was developed optimized using limiting dilutions cell line mixed normal specimens. The test then used to examine samples obtained from 15 abdominal carcinomas (colorectal, n = 10;...
Biliary glycoprotein (BGP) is the human homologue of a cell adhesion molecule (CAM) rat designated Cell-CAM. The BGP gene member carcinoembryonic antigen family, which belongs to immunoglobulin superfamily. expressed in cells epithelial and myeloid origin. In granulocytes, main CD66 cluster differentiation antigens that mediate binding endothelial E-selectin. Since major CAM, expression was studied 21 colorectal carcinoma tissue specimens respective adjacent normal mucosae. As an internal...
CD66a, also called biliary glycoprotein (BGP), is a member of the carcinoembryonic antigen (CEA) family and immunoglobulin superfamily. CD66a human homologue Cell-CAM, well-defined cell adhesion molecule rat. In present study monoclonal antibody specific for was used to locate in tissues. expressed epithelia, certain endothelia, cells myeloid lineage. Hepatocytes were stained along bile canaliculi. A characteristic apical membranous staining observed enterocytes, superficial absorptive...
The prognosis of digestive cancers is poor mainly due to intraperitoneal relapse by cells which may have already been seeded at the time surgery. Using immunocytology we investigated peritoneal cavity and, as a comparison, bone marrow 147 patients with gastric, colorectal and pancreatic cancer for micrometastatic cells. Cytological samples from lavages aspirates were analyzed using monoclonal antibodies (MAbs) against tumor-associated antigens (TAA) (CEA, CA-19-9, 17-1-A, C-54-0, Ra96)...
OBJECTIVES: Autoimmune pancreatitis (AIP) is thought to be an immune-mediated inflammatory process, directed against the epithelial components of pancreas. The objective was identify novel markers disease and unravel pathogenesis AIP. METHODS: To explore key targets we analyzed expression proteins at RNA protein level using genomics proteomics, immunohistochemistry, western blot, immunoassay. An animal model AIP with LP-BM5 murine leukemia virus-infected mice studied in parallel. microarrays...
Abstract. Purpose: To measure the concentration of cytokines in aqueous humour eyes with exudative age‐related macular degeneration (AMD). Methods: The clinical interventional study included a group 18 patients AMD and control 20 undergoing routine cataract surgery. Age did not vary significantly (p = 0.36) between (80.8 ± 6.4 years) (77.0 9.9 years), nor gender 0.75). During interventions, samples were obtained, which was measured using solid‐phase chemiluminescence immunoassay. Macular...
The aim of this study was to prospectively evaluate the accuracy quantitative cardiac computed tomography (CT) parameters and two biomarkers (N-terminal-pro-brain natriuretic peptide (NT-pro-BNP) troponin I), alone in combination, for predicting right ventricular dysfunction (RVD) patients with acute pulmonary embolism. 557 consecutive suspected embolism underwent CT angiography. Patients also echocardiography NT-pro-BNP/troponin I serum level measurements. Three different measurements were...
Summary Introduction: This article is part of the Focus Theme Methods Information in Medicine on German Medical Informatics Initiative. Similar to other large international data sharing networks (e.g. OHDSI, PCORnet, eMerge, RD-Connect) MIRACUM a consortium academic and hospital partners as well one industrial partner eight cities which have joined forces create interoperable integration centres (DIC) make within those DIC available for innovative new IT solutions patient care medical...
To measure cytokine concentrations in aqueous humor of eyes with diffuse diabetic macular edema.The interventional clinical comparative study included a group 23 patients edema and control 22 undergoing cataract surgery. Cytokine were measured samples using Luminex xMAP suspension array technology.In the as compared group, significantly higher for epidermal growth factor (P < 0.001), human intercellular adhesion molecule-1 (ICAM-1; P interleukin (IL)-1a2 = 0.04), IL-6 IL-8 interferon...
The reverse transcription polymerase chain reaction (RT-PCR) amplification of cytokeratin 20 (CK20) mRNA is considered a promising candidate method for the detection circulating tumour cells in bone marrow and peripheral blood cancer patients. In this study we have investigated diagnostic specificity CK20 samples from healthy donors (HD; n = 33), intensive care units patients (ICU; 20) obtained suffering chronic inflammatory diseases (CID; 14). RNAs purified stabilized lysates showed...
We have isolated and sequenced four overlapping cDNA clones from a normal adult human colon library, which together gave the entire nucleotide sequence for biliary glycoprotein I (BGP I). BGP is member of carcinoembryonic antigen (CEA) gene family, subfamily in immunoglobulin superfamily. The deduced amino acid combined revealed 34-residue leader followed by 108-residue N-terminal domain, 178-residue immunoglobulin-like region specific to I, 24-residue transmembrane 35-residue cytoplasmic...
Carnosinase 1 (CN1) is a secreted dipeptidase that hydrolyzes L-carnosine. Recently, we have identified an allelic variant of human CN1 (hCN1) results in increased enzyme activity and associated with susceptibility for diabetic nephropathy patients. We therefore hypothesized L-carnosine the serum represents critical protective factor patients.L-carnosine levels were manipulated db/db mice, model type 2 diabetes. In transgenic approach, hCN1 cDNA was expressed under control liver-specific...
NCA (nonspecific cross-reacting antigen), a glycoprotein found in normal lung and spleen, is immunologically related to carcinoembryonic antigen (CEA), which over 95% of colon adenocarcinomas. From human genomic library, we previously cloned part an gene showed that the amino-terminal region has extensive sequence homology CEA (Thompson, J. A., Pande, H., Paxton, R. J., Shively, L., Padma, Simmer, Todd, Ch. W., Riggs, A. D., J.E. (1987) Proc. Natl. Acad. Sci. U. S.A. 84, 2965-2969). We now...
Currently known prognostic serum biomarkers of melanoma are powerful in metastatic disease, but weak early-stage patients. This study was aimed to identify new by mass spectrometry (MS) proteomic profiling, and validate candidates compared with established markers.Two independent sets samples from 596 patients were investigated. The first set (stage I = 102; stage IV 95) analyzed matrix assisted laser desorption ionization time flight (MALDI TOF) MS for differentiating between IV. In the...
Background Micro-CT imaging of liver disease in mice relies on high soft tissue contrast to detect small lesions like metastases. Purpose this study was characterize the localization and time course enhancement a nanoparticular alkaline earth metal-based agent (VISCOVER ExiTron nano) developed for animal CT imaging. Methodology nano 6000 12000, formulated liver/spleen angiography, respectively, were intravenously injected C57BL/6J-mice. The distribution analysed by repeated micro-CT up 6...