A5029054992- Genetic factors in colorectal cancer
- Radiopharmaceutical Chemistry and Applications
- Hepatocellular Carcinoma Treatment and Prognosis
- Peptidase Inhibition and Analysis
- Liver Disease Diagnosis and Treatment
- Colorectal Cancer Treatments and Studies
- Liver physiology and pathology
- Monoclonal and Polyclonal Antibodies Research
- Advanced X-ray and CT Imaging
- Glycosylation and Glycoproteins Research
- Pituitary Gland Disorders and Treatments
- MRI in cancer diagnosis
- Biosimilars and Bioanalytical Methods
- Airway Management and Intubation Techniques
- Pediatric Hepatobiliary Diseases and Treatments
- Hormonal and reproductive studies
- Chronic Lymphocytic Leukemia Research
- Biomedical Research and Pathophysiology
- Protein purification and stability
- Growth Hormone and Insulin-like Growth Factors
- Lung Cancer Diagnosis and Treatment
- Respiratory Support and Mechanisms
- Ultrasound and Hyperthermia Applications
- TGF-β signaling in diseases
- Parathyroid Disorders and Treatments
University Medical Centre Mannheim
2010-2024
Heidelberg University
2011-2024
University Hospital Heidelberg
2008-2024
Robert Koch Institute
2023
Medizinische Fakultät Mannheim
2010
Background Micro-CT imaging of liver disease in mice relies on high soft tissue contrast to detect small lesions like metastases. Purpose this study was characterize the localization and time course enhancement a nanoparticular alkaline earth metal-based agent (VISCOVER ExiTron nano) developed for animal CT imaging. Methodology nano 6000 12000, formulated liver/spleen angiography, respectively, were intravenously injected C57BL/6J-mice. The distribution analysed by repeated micro-CT up 6...
TGF-β2 (TGF-β, transforming growth factor beta), the less-investigated sibling of TGF-β1, is deregulated in rodent and human liver diseases. Former data from bile duct ligated MDR2 knockout (KO) mouse models for cholestatic disease suggested an involvement biliary-derived As we also found upregulated TGFB2 tissue patients with primary sclerosing cholangitis (PSC) biliary (PBC), now fathomed positive prospects targeting early stage using MDR2-KO mice. Specifically, influence TgfB2 silencing...
Various murine models are currently used to study acute and chronic pathological processes of the liver, efficacy novel therapeutic regimens. The increasing availability high-resolution small animal imaging modalities presents researchers with opportunity precisely identify describe liver. To meet demands, objective this was provide a three-dimensional illustration macroscopic anatomical location liver lobes hepatic vessels using modalities. We analysed micro-CT images by integrating...
Significance Bone is the main source of fibroblast growth factor 23 (FGF23), which important for phosphate and vitamin D homeostasis. In acute kidney injury (AKI), high blood levels FGF23 are positively correlated with disease progression increased risk mortality. Reducing adverse plasma in AKI patients favorable. We showed here that hepatocytes major circulating FGF23, orphan nuclear receptor ERR-γ a novel transcriptional regulator hepatic production AKI. Liver-specific depletion or inverse...
Abstract Internal image anti-idiotypic antibodies are capable of mimicking tumor-associated antigens and thus may serve as surrogate for vaccination strategies in cancer patients. The monoclonal antibody (mAb) 6G6.C4 mimics an epitope specific the human carcinoembryonic antigen (CEA) generates a CEA-specific response (Ab3) various experimental animals. In humans, however, only yields very limited humoral anti-CEA reaction presumably due to tolerance against CEA autoantigen. this study, we...
To analyze the performance of 1 mg dexamethasone suppression test (DST) in patients with obesity. Special attention was paid to influence interfering medication on DST.In this prospective cohort study (Mannheim Obesity Study), obesity were evaluated before bariatric surgery. For evaluation hypercortisolism, a dexamethasone-suppression all subjects performed. Medication assessed for possible interference.Two hundred seventy-eight mean age 42.3 years (68.8% women) and BMI 47.9 ± 8.4 kg/m(2)...
Colorectal cancer is one of the most common forms cancer, and development novel tools for detection efficient treatment metastases needed. One promising approach use radiolabeled antibodies positron emission tomography (PET) imaging radioimmunotherapy. Since carcinoembryonic antigen (CEA) an important target in colorectal CEA-specific M5A antibody has been extensively studied subcutaneous xenograft models; however, not yet tested advanced models liver metastases. The aim this study was to...
Respiratory gating with and without controlled ventilation has been applied for in vivo micro-computed tomography (micro-CT) of thoracic abdominal structures mice. We describe a simplified method intubation demonstrate its applicability single-breath-hold micro-CT mice.Mice (n = 10) were anesthetized, intubated, ventilated, relaxed by intraperitoneal administration rocuronium. Contrast-enhanced the complete thorax including upper organs (80 kV; 37.5 μA; 190-degree rotation; 600...
Separase, an endopeptidase required for the separation of sister-chromatides in mitotic anaphase, triggers centriole disengagement during centrosome duplication. In cancer, separase is frequently overexpressed, pointing to a functional role as aneuploidy promoter associated with centrosomal amplification and genomic instability. Recently, we have shown that subsequent chromosomal aberrations are hallmark chronic myeloid leukemia (CML), increasing from phase (CP) toward blast crisis (BC)....
Abstract Background Intranasal (i.n.) drug application is a widely known and low-invasive route of administration that may be able to achieve rapid symptom control in terminally ill patients. According the German S3 guideline “Palliative care for patients with incurable cancer”, benzodiazepines, such as midazolam, are recommended treatment terminal agitation. To best our knowledge there no evidence i.n. midazolam We aim assess use an alternative subcutaneous drug. Methods In this...
Abstract Objective Pituitary apoplexy is a serious medical complication of pre-existing pituitary adenoma characterized by variety clinical symptoms ranging from mild headache to neurologically impaired and finally comatose patients. Management options are surgery or conservative treatment (e. g., with dexamethasone). Surgery commonly performed in case severe acute neurological visual symptoms. However, prospective studies demonstrating benefit over terms visual, even endocrine outcomes...
According to their carcinogenesis, colorectal cancer (CRC) subtypes show distinct molecular parameters. Hereditary non-polypous (HNPCC) is the most common inherited CRC characterized by clinical criteria and confirmed microsatellite instability (MSI). Interestingly, a recently identified subtype, familial type X (FCC-X), shows same but stability (MSS). CEACAM1 known tumor suppressor that regulates apoptosis in colon cells, its loss one of frequent events early tumorigenesis CRC. Therefore...
This report demonstrates a novel class of innate immune cells designated "variable immunoreceptor-expressing myeloids" (VIREMs). Using single-cell transcriptomics and genome-wide epigenetic profiling, we establish that VIREMs are myeloid unrelated to lymphocytes. We visualize the phenotype B-VIREMs capable genetically recombining expressing antibody genes, exclusive hallmark function B These cells, B-VIREMs, display monoclonal cell surface signatures regularly circulate in blood healthy...
TGF-β1 is a pro-fibrogenic cytokine in chronic liver diseases (CLD), driving fibrogenesis. Many experimental approaches to tackle fibrogenesis were directed towards and provided promising results. However, none of these could be translated into clinical trials for patient treatment up now. In contrast, have been conducted that against TGF-β2.
Confirming relevant performance characteristics (i.e.verification) of laboratory systems for their implementation in routine diagnostics is crucial quality assurance clinical laboratory.Thereby, a diagnostic method or analyzer proven being suitable fulfilling tasks with far-reaching decisions regarding patients' health as well economic processes laboratory.Therefore, variety aspects must be taken into account during the practical and system verification transfer diagnostics. Aims Methods:The...
Zielsetzung: Die Mikro-CT erlaubt die non-invasive Bildgebung der thorakalen und abdominellen Organe von Versuchstieren. Als Alternative zur herkömmlichen Methode des Atemgatings beschreiben wir eine vereinfachte Intubation Mäusen evaluieren den Einsatz einer Atemstopptechnik für ultra-schnelle Thorax Abdomen.