A5050541523- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Cancer Genomics and Diagnostics
- Immune Cell Function and Interaction
- Immune cells in cancer
- Acute Myeloid Leukemia Research
- Telomeres, Telomerase, and Senescence
- Genetic factors in colorectal cancer
- Chronic Myeloid Leukemia Treatments
- COVID-19 Clinical Research Studies
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Biosimilars and Bioanalytical Methods
- Immune Response and Inflammation
- Long-Term Effects of COVID-19
- Signaling Pathways in Disease
- T-cell and B-cell Immunology
- Radiomics and Machine Learning in Medical Imaging
- S100 Proteins and Annexins
- Cancer, Hypoxia, and Metabolism
- Melanoma and MAPK Pathways
- Traumatic Brain Injury and Neurovascular Disturbances
- Gene expression and cancer classification
- RNA regulation and disease
- Hedgehog Signaling Pathway Studies
- Liver physiology and pathology
Heidelberg University
2012-2025
University Hospital Heidelberg
2020-2025
University Medical Centre Mannheim
2020-2024
Jena University Hospital
2016-2020
Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. - Hans-Knöll-Institut (HKI)
2018-2019
German Cancer Research Center
2012
Medulloblastoma is the most common brain tumour in children; using whole-genome sequencing of samples authors show that clinically challenging Group 3 and 4 tumours can be tetraploid, reveal expression first medulloblastoma fusion genes identified. malignant children. Four papers published 2 August 2012 issue Nature use other techniques to produce a detailed picture genetics genomics this condition. Notable findings include identification recurrent mutations not previously implicated...
Abstract Disease epidemiology during ageing shows a transition from cancer to degenerative chronic disorders as dominant contributors mortality in the old. Nevertheless, it has remained unclear what extent molecular signatures of reflect this phenomenon. Here we report on identification conserved transcriptomic signature based gene expression data four vertebrate species across tissues. We find that ageing-associated changes follow trajectories similar transcriptional alterations observed...
IntroductionThe longevity of antibody levels against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and the duration immunity are current topics major scientific interest. Antibody kinetics during phase well studied, whereas long-term yet to be determined, with contradictory results from studies date. Here, we present a longitudinal analysis serological responses SARS-CoV-2 infection following convalescence association post-COVID (PCS).Materials methodsA total 237 serum samples...
Immunotherapies for malignant melanoma are challenged by the resistance developed in a significant proportion of patients. Myeloid-derived suppressor cells (MDSC), with their ability to inhibit antitumor T-cell responses, major contributor immunosuppression and immune checkpoint therapies melanoma. Damage-associated molecular patterns S100A8, S100A9, HMGB1, acting as toll like receptor 4 (TLR4) advanced glycation endproducts (RAGE) ligands, highly expressed tumor microenvironment drive MDSC...
Liver sinusoidal endothelial cells (LSECs) rapidly clear lipopolysaccharide (LPS) from the bloodstream and establish intimate contact with immune cells. However, their role in regulating liver inflammation remains poorly understood. We show that LSECs modify chemokine expression profile driven by LPS or interferon-γ (IFN-γ), resulting production of myeloid- lymphoid-attracting chemokines CCL2 CXCL10, respectively, which accumulate serum LPS-challenged animals. Natural killer (NK) cell...
Abstract Objectives Assessment of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection prevalence and immunity is cornerstones in the fight against COVID-19 pandemic. For pandemic control, reliable assays for detection anti-SARS-CoV-2 antibodies are required. This pilot external quality assessment (EQA) scheme aimed to independently assess participants’ clinical performance testing, identify shortcomings practice evaluate suitability format. Methods The EQA consisted eight...
Melanoma is a cancer with rising incidence and new therapeutics are needed. For this, it necessary to understand the molecular mechanisms of melanoma development progression. differs from other cancers by its ability produce pigment melanin via melanogenesis; this biosynthesis essentially regulated microphthalmia-associated transcription factor (MITF). MITF regulates various processes such as cell cycling differentiation. shows an ambivalent role, since high levels inhibit proliferation low...
Colorectal cancer remains a leading cause of cancer-related death worldwide. A previous transcriptomics based study characterized molecular subgroups which the stromal subgroup was associated with worst clinical outcome. Micro-RNAs (miRNAs) are well-known regulators gene expression and can follow non-linear repression mechanism. We set up model combining piecewise linear regression applied this combined to comprehensive colon adenocarcinoma dataset. identified miRNAs involved in regulating...
Serum biomarker S100B has been explored for its potential benefit to improve clinical decision-making in the management of patients suffering from traumatic brain injury (TBI), especially as a pre-head computed-tomography screening test with mild TBI. Although being already included into some guidelines, implementation standard care is still lacking. This might be explained by turnaround time (TAT) too long serum used emergency settings.S100B concentrations were determined 136 matching pairs...
The TrkA receptor tyrosine kinase induces death in medulloblastoma cells via an interaction with the cerebral cavernous malformation 2 (CCM2) protein. We used affinity proteomics to identify germinal center class III (GCKIII) kinases STK24 and STK25 as novel CCM2 interactors. Down-modulation of STK25, but not STK24, rescued from NGF-induced TrkA-dependent cell death, suggesting that is part death-signaling pathway initiated by CCM2. can be phosphorylated activity required for signaling....
The analysis of circulating tumor DNA (ctDNA) is at the threshold implementation into standard care for colorectal cancer (CRC) patients. However, data about clinical utility liquid profiling (LP), its acceptance by clinicians, and integration workflows in real‐world settings remain limited. Here, LP tests requested as part routine since 2016 were retrospectively evaluated. Results show restrained request behavior that improved moderately over time, well reliable diagnostic performance...
External quality assessment (EQA) is a key instrument for achieving harmonization, and thus high quality, of diagnostic procedures. As reliable test results are crucial accurate SARS-CoV-2 infection prevalence, vaccine response, immunity, successful management the ongoing COVID-19 pandemic, Reference Institute Bioanalytics (RfB) was first EQA provider to offer an open scheme anti-SARS-CoV-2 antibody detection.
During the last two years, a variety of assays for serological detection antibodies to new SARS-CoV-2 virus have been launched and used as part standard care in many laboratories. The pace with which these tests introduced into routine emphasizes importance quality measures analytical methods, particularly regard implications results clinical epidemiologic decisions. Accuracy, reliability comparability test are thus essential, here external assessment (EQA) is most important assurance tool....
The duration of anti-SARS-CoV-2-antibody detectability up to 12 months was examined in individuals after either single convalescence or and vaccination. Moreover, variables that might influence an anti-RBD/S1 antibody decline the existence a post-COVID-syndrome (PCS) were addressed.Forty-nine SARS-CoV-2-qRT-PCR-confirmed participants completed 12-month examination levels PCS-associated long-term sequelae. Overall, 324 samples collected. Cell-free DNA (cfDNA) isolated quantified from...
Mucormycosis is an emergent, fatal fungal infection of humans and warm-blooded animals caused by species the order Mucorales. Immune cells innate immune system serve as first line defence against inhaled spores. Alveolar macrophages were challenged with mucoralean fungus Lichtheimia corymbifera subjected to biotinylation streptavidin enrichment procedures followed LC-MS/MS analyses. A total 28 host proteins enriched for binding macrophage-L. interaction. Among those, HSP70-family protein...
Reactivation of the telomerase reverse transcriptase gene TERT is a central feature for unlimited proliferation majority cancers. However, underlying regulatory processes are only partly understood.We assembled regulator binding information from serveral sources to construct generic human and mouse network. Advancing our "Mixed Integer linear Programming based Regulatory Interaction Predictor" (MIPRIP) approach, we identified most common cancer-type specific regulators across 19 different...
Severe courses and high hospitalization rates were ubiquitous during the first pandemic SARS-CoV-2 waves. Thus, we aimed to examine whether integrative diagnostics may aid in identifying vulnerable patients using crucial data materials obtained from COVID-19 hospitalized between 2020 2021 (n = 52). Accordingly, investigated potential of laboratory biomarkers, specifically dynamic cell decay marker cell-free DNA radiomics features extracted chest CT.Separate forward backward feature selection...
This report demonstrates a novel class of innate immune cells designated "variable immunoreceptor-expressing myeloids" (VIREMs). Using single-cell transcriptomics and genome-wide epigenetic profiling, we establish that VIREMs are myeloid unrelated to lymphocytes. We visualize the phenotype B-VIREMs capable genetically recombining expressing antibody genes, exclusive hallmark function B These cells, B-VIREMs, display monoclonal cell surface signatures regularly circulate in blood healthy...
Acute myeloid leukaemia (AML) is a haematopoietic malignancy caused by combination of genetic and epigenetic lesions. Activation the oncoprotein FLT3 ITD (Fms-like tyrosine kinase with internal tandem duplications) represents key driver mutation in 25–30% AML patients. class III receptor kinase, which plays role cell survival, proliferation, differentiation progenitors lymphoid lineages. Mutant results an altered signalling quality, causes transformation. Recent evidence indicates effect on...