A5054049840- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Liver physiology and pathology
- Advanced Chemical Sensor Technologies
- Wound Healing and Treatments
- Essential Oils and Antimicrobial Activity
- Infectious Diseases and Mycology
- Pancreatic function and diabetes
- Liver Disease Diagnosis and Treatment
- Microbial Metabolic Engineering and Bioproduction
- Fungal and yeast genetics research
- Antimicrobial Resistance in Staphylococcus
- Chromium effects and bioremediation
- Infections and bacterial resistance
- Clostridium difficile and Clostridium perfringens research
- Olfactory and Sensory Function Studies
- Biofuel production and bioconversion
- Fungal Biology and Applications
- Monoclonal and Polyclonal Antibodies Research
- Bacterial biofilms and quorum sensing
- Immunodeficiency and Autoimmune Disorders
- Arsenic contamination and mitigation
Jena University Hospital
2022-2024
Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. - Hans-Knöll-Institut (HKI)
2017-2021
National Research Centre
2018-2021
Friedrich Schiller University Jena
2018-2021
Scarcity of the non-renewable energy sources, global warming, environmental pollution, and raising cost petroleum are motive for development renewable, eco-friendly fuels production with low costs. Bioethanol is one promising materials that can subrogate oil, it considered recently as a clean liquid fuel or neutral carbon. Diverse microorganisms such yeasts bacteria able to produce bioethanol on large scale, which satisfy our daily needs cheap applicable methods. Saccharomyces cerevisiae...
Metabolic dysfunction-associated steatotic liver disease (MASLD) is hallmarked by hepatic steatosis, cell injury, inflammation, and fibrosis. This study elaborates on a multicellular biochip-based sinusoid model to mimic MASLD pathomechanisms investigate the therapeutic effects of drug candidates lanifibranor resmetirom. Mouse primary hepatocytes, stellate cells, Kupffer endothelial cells are seeded in dual-chamber biocompatible liver-on-a-chip (LoC). The LoC then perfused with circulating...
Infections with Staphylococcus aureus (S. aureus) have been reported from various organs ranging asymptomatic colonization to severe infections and sepsis. Although considered an extracellular pathogen, S. can invade persist in professional phagocytes such as monocytes macrophages. Its capability manipulate macrophages is a critical step evade host antimicrobial reactions. We leveraged recently established human liver-on-chip model demonstrate that specifically targets essential niche...
Iron is an essential micronutrient for most organisms and fungi are no exception. uptake by facilitated receptor-mediated internalization of siderophores, heme reductive iron assimilation (RIA). The RIA employs three protein groups: (i) the ferric reductases (Fre5 proteins), (ii) multicopper ferroxidases (Fet3) (iii) high-affinity permeases (Ftr1). Phenotyping under different concentrations revealed detrimental effects on spore swelling hyphal formation depletion, but yeast-like morphology...
Phagocytosis is series of steps where the pathogens and immune cells interact during an invasion. This starts with adhesion process between host pathogen cells, followed by engulfment pathogens. Many analytical methods that are applied to characterize phagocytosis based on imaging host-pathogen confrontation assays rely fluorescence labeling cells. However, potential effect membrane quantitative results has not been studied in detail. In this study, we determine whether processes themselves...
Summary Mucormycoses are life‐threatening infections that affect patients suffering from immune deficiencies. We performed phagocytosis assays confronting various strains of Lichtheimia species with alveolar macrophages, which form the first line defence innate system. To investigate 17 four different continents in a comparative fashion, transmitted light and confocal fluorescence microscopy was applied combination automated image analysis. This interdisciplinary approach enabled objective...
Fungal infections caused by the ancient lineage Mucorales are emerging and increasingly reported in humans. Comprehensive surveys on promising attributes from a multitude of possible virulence factors limited so far, focused Mucor Rhizopus. This study addresses systematic approach to monitor phagocytosis after physical enzymatic modification outer spore wall Lichtheimia corymbifera, one major causative agents mucormycosis. Episporic modifications were performed their consequences...
Mucormycosis is an emergent, fatal fungal infection of humans and warm-blooded animals caused by species the order Mucorales. Immune cells innate immune system serve as first line defence against inhaled spores. Alveolar macrophages were challenged with mucoralean fungus Lichtheimia corymbifera subjected to biotinylation streptavidin enrichment procedures followed LC-MS/MS analyses. A total 28 host proteins enriched for binding macrophage-L. interaction. Among those, HSP70-family protein...